公开(公告)号：US06515280B1

公开(公告)日：2003-02-04

申请号：US09525234

申请日：2000-03-15

Applicant: Gökhan Baykut

Inventor： Gökhan Baykut

IPC: H01J4904

CPC classification number: H01J49/164 ,  H01J49/0481

Abstract: A matrix assisted laser desorption ionization (MALDI) source with the capability of exposing the sample to a local gas pulse exactly at the point where the laser desorption process occurs, helps reduce the excess kinetic energy of the generated ions. Thus, it allows a better control of the ions during their transfer to and capture in ion trap mass spectrometers. Avoiding the unnecessary pressure increase, the MALDI source helps reduce pump-off times and the performance decrease particularly in Fourier transformation ion cyclotron resonance mass spectrometers. In addition, it allows the use of pulsed reactive gases in connection with direct or matrix assisted laser desorption, which can lead to the formation of novel product ions. The MALDI source also provides the capability of capturing and accumulating the ions in an in-source multipole ion guide for increasing the sensitivity of the method.

Abstract translation: 具有将样品暴露于激光解吸过程正好位置的局部气体脉冲的能力的基质辅助激光解吸电离（MALDI）源有助于减少产生的离子的过量动能。 因此，它可以更好地控制离子在离子阱质谱仪中的转移和捕获。 避免不必要的压力增加，MALDI源有助于减少泵浦时间，特别是在傅立叶变换离子回旋共振质谱仪中性能下降。 此外，它允许使用与直接或基体辅助激光解吸相关的脉冲反应气体，这可导致新产物离子的形成。 MALDI源还提供了在源极多极离子导子中捕获和积聚离子的能力，以提高该方法的灵敏度。

公开(公告)号：US5206509A

公开(公告)日：1993-04-27

申请号：US805442

申请日：1991-12-11

Applicant: Scott A. McLuckey ,  Douglas E. Goeringer ,  Gary L. Glish

Inventor： Scott A. McLuckey ,  Douglas E. Goeringer ,  Gary L. Glish

IPC: H01J49/38 ,  H01J49/42

CPC classification number: H01J49/005 ,  H01J49/0481

Abstract: A universal collisional activation ion trap comprises an ion trapping means containing a bath gas and having connected thereto a noise signal generator. A method of operating a universal collisional activation ion trap comprises the steps of: providing an ion trapping means; introducing into the ion trapping means a bath gas; and, generating a noise signal within the ion trapping means; introducing into the ion trapping means a substance that, when acted upon by the noise signal, undergoes collisional activation to form product ions.

Abstract translation: 通用碰撞激活离子阱包括含有浴气并已连接到其上的噪声信号发生器的离子捕获装置。 操作普通碰撞激活离子阱的方法包括以下步骤：提供离子捕获装置; 引入离子捕获是指浴气; 并且在所述离子俘获装置内产生噪声信号; 引入离子捕获是指当被噪声信号作用时经历碰撞激活以形成产物离子的物质。

公开(公告)号：US20240145027A1

公开(公告)日：2024-05-02

申请号：US18567573

申请日：2022-06-01

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

Inventor： Priyanka BANSAL ,  Ahmed BEN FALEH ,  Robert PELLEGRINELLI ,  Stephan WARNKE ,  Thomas RIZZO

IPC: G16B15/00 ,  G01N21/35 ,  G01N27/623 ,  G01N33/68 ,  G16B40/10 ,  H01J49/04

CPC classification number: G16B15/00 ,  G01N21/35 ,  G01N27/623 ,  G01N33/6851 ,  G16B40/10 ,  H01J49/0481 ,  G01N2201/06113

Abstract: A method, system, and computer-readable medium for identifying and creating a database of isomers and isobars of molecules including the steps of performing isomer or isobar separation on molecules to obtain separate isomeric or isobaric molecules, measuring mass-to-charge ratios (m/z) to obtain IR fingerprints of the separate isomeric or isobaric molecules, and storing first data on the mass-to-charge ratios (m/z) and/or the IR fingerprints of the separate isomeric or isobaric molecules to a database.

公开(公告)号：US20180240657A1

公开(公告)日：2018-08-23

申请号：US15752835

申请日：2016-08-11

Applicant: Thermo Fisher Scientific (Bremen) GmbH

Inventor： Johannes SCHWIETERS

IPC: H01J49/00 ,  H01J49/04 ,  H01J49/06 ,  H01J49/30

CPC classification number: H01J49/004 ,  H01J49/0481 ,  H01J49/063 ,  H01J49/30

Abstract: The invention provides a mass spectrometer that comprises a collision cell having an axial electric field that enhances transmission of light ions, especially elemental ions, through the collision cell, relative to heavier ions. The invention also provides methods of mass spectrometry that employ an axial electric field that is provided in a collision cell.

公开(公告)号：US09368335B1

公开(公告)日：2016-06-14

申请号：US14611682

申请日：2015-02-02

Applicant: Thermo Finnigan LLC

Inventor： Scott T. Quarmby ,  Berg A. Tehlirian

IPC: H01J49/24 ,  H01J49/04 ,  F04D19/04

CPC classification number: H01J49/24 ,  F04D19/042 ,  F04D19/046 ,  H01J49/0422 ,  H01J49/0481

Abstract: A mass spectrometer system can include a vacuum manifold and a high vacuum pump. The vacuum manifold can include a foreline chamber and a high vacuum chamber. The foreline chamber can have a source inlet, a foreline inlet, and a foreline outlet. The high vacuum pump can have a vacuum port coupled to high vacuum chamber, and a foreline port coupled to the foreline inlet.

Abstract translation: 质谱仪系统可以包括真空歧管和高真空泵。 真空歧管可以包括前排室和高真空室。 前排室可以具有源入口，前级管道入口和前级管路出口。 高真空泵可以具有联接到高真空室的真空端口和联接到前级管线入口的前级管路端口。

公开(公告)号：US20160141167A1

公开(公告)日：2016-05-19

申请号：US15005881

申请日：2016-01-25

Applicant: Thermo Fisher Scientific (Bremen) GmbH

Inventor： Alexander MAKAROV ,  Eduard V. DENISOV ,  Wilko BALSCHUN ,  Dirk NOLTING ,  Jens GRIEP-RAMING

IPC: H01J49/04 ,  H01J49/00

CPC classification number: H01J49/0422 ,  H01J49/0031 ,  H01J49/0045 ,  H01J49/0072 ,  H01J49/0081 ,  H01J49/0481 ,  H01J49/06 ,  H01J49/26 ,  H01J49/40

Abstract: A method of operating a gas-filled collision cell in a mass spectrometer is provided. The collision cell has a longitudinal axis. Ions are caused to enter the collision cell. A trapping field is generated within the collision cell so as to trap the ions within a trapping volume of the collision cell, the trapping volume being defined by the trapping field and extending along the longitudinal axis. Trapped ions are processed in the collision cell and a DC potential gradient is provided, using an electrode arrangement, resulting in a non-zero electric field at all points along the axial length of the trapping volume so as to cause processed ions to exit the collision cell. The electric field along the axial length of the trapping volume has a standard deviation that is no greater than its mean value.

公开(公告)号：US09263223B2

公开(公告)日：2016-02-16

申请号：US13870166

申请日：2013-04-25

Applicant: Bruker Daltonik GmbH

Inventor： Michael Duerr ,  Christoph Gebhardt

IPC: H01J7/24 ,  H05B31/26 ,  H01J27/02 ,  H01J49/04 ,  H01J49/14

CPC classification number: H01J27/026 ,  H01J49/0481 ,  H01J49/142

Abstract: The invention relates to devices and methods in mass spectrometers for the generation of ions of heavy molecules, especially biomolecules, by bombarding them with uncharged clusters of molecules. The analyte ions which are generated or released by cluster bombardment of analyte substances on the surface of sample support plates show a broad distribution of their kinetic energies, which prevents good ion-optical focusing. In the invention, the kinetic energies are homogenized in a higher-density collision gas. The collision gas is preferably located in an RF ion guide, more preferably an RF ion funnel, which can transfer the ions to the mass analyzer. The collision gas may be introduced with temporal pulsing, coordinated or synchronized with the pulsed supersonic gas jet. The collision gas may be pumped off again before the next supersonic gas pulse. In an advantageous embodiment, the collision gas can originate from the supersonic gas jet itself.

Abstract translation: 本发明涉及用于通过用不带电荷的分子团轰击它们来产生重分子，特别是生物分子的离子的质谱仪中的装置和方法。 通过在样品支撑板表面上的分析物质的聚集轰击产生或释放的分析物离子显示出其动能的广泛分布，这阻止了良好的离子光学聚焦。 在本发明中，动能在较高密度的碰撞气体中均质化。 碰撞气体优选位于RF离子导向件中，更优选地位于可以将离子转移到质量分析器的RF离子漏斗中。 碰撞气体可以与脉冲超音速气体射流一起引入时间脉冲，协调或同步。 碰撞气体可能在下一个超音速气体脉冲之前被再次泵出。 在有利的实施例中，碰撞气体可以源于超音速气体射流本身。

公开(公告)号：US20150364308A1

公开(公告)日：2015-12-17

申请号：US14834291

申请日：2015-08-24

Applicant: Thermo Fisher Scientific (Bremen) GmbH

Inventor： Alexander MAKAROV ,  Eduard V. DENISOV ,  Wilko BALSCHUN ,  Dirk NOLTING ,  Jens GRIEP-RAMING

IPC: H01J49/04 ,  H01J49/00

CPC classification number: H01J49/0422 ,  H01J49/0031 ,  H01J49/0045 ,  H01J49/0072 ,  H01J49/0081 ,  H01J49/0481 ,  H01J49/06 ,  H01J49/26 ,  H01J49/40

Abstract: A method of operating a gas-filled collision cell in a mass spectrometer is provided. The collision cell has a longitudinal axis. Ions are caused to enter the collision cell. A trapping field is generated within the collision cell so as to trap the ions within a trapping volume of the collision cell, the trapping volume being defined by the trapping field and extending along the longitudinal axis. Trapped ions are processed in the collision cell and a DC potential gradient is provided, using an electrode arrangement, resulting in a non-zero electric field at all points along the axial length of the trapping volume so as to cause processed ions to exit the collision cell. The electric field along the axial length of the trapping volume has a standard deviation that is no greater than its mean value.

Abstract translation: 提供了在质谱仪中操作充气碰撞池的方法。 碰撞单元具有纵向轴线。 导致离子进入碰撞池。 在碰撞单元内产生捕获场，以便将离子捕获在碰撞池的捕获体积内，捕获体积由捕获场限定并沿着纵轴延伸。 捕获的离子在碰撞单元中被处理，并且使用电极布置提供DC电位梯度，导致沿着捕获体积的轴向长度的所有点处的非零电场，从而使经处理的离子离开碰撞 细胞。 沿着捕集体积的轴向长度的电场具有不大于其平均值的标准偏差。

公开(公告)号：US20150287583A1

公开(公告)日：2015-10-08

申请号：US14746753

申请日：2015-06-22

Applicant: Thermo Fisher Scientific (Bremen) GmbH

Inventor： Alexander Alekseevich MAKAROV

IPC: H01J49/10 ,  H01J49/40

CPC classification number: H01J49/10 ,  G01N27/622 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/0481 ,  H01J49/06 ,  H01J49/063 ,  H01J49/401 ,  H01J49/4215 ,  H01J49/423 ,  H01J49/425 ,  H01J49/426 ,  H01J49/427 ,  H01J49/4295

Abstract: An ion spectrometer is provided, comprising: an ion source, arranged to generate ions continuously with a first range of mass to charge ratios; and an ion trap, arranged to receive ions from the ion source along an axis, and to eject ions with a second range of mass to charge ratios orthogonally to that axis, the second range of mass to charge ratios being narrower than the first range of mass to charge ratios. In some embodiments, ions generated by the ion source continuously flow into the ion trap. Additionally or alternatively, ion optics receive ions ejected from the ion trap and cool the ions without substantial fragmentation. An ion analyser receives ions ejected from the ion trap or ion optics and separates the ions in accordance with at least one characteristic of the ions.

Abstract translation: 提供了一种离子光谱仪，包括：离子源，被布置成以第一质量与电荷比例连续地产生离子; 以及离子阱，被布置成沿着轴接收离子源的离子，并且以与该轴正交的质量与第二质量范围的质量与电荷的比率排出离子，质量与电荷比的第二范围比第一范围的 质荷比。 在一些实施例中，由离子源产生的离子连续地流入离子阱。 另外或替代地，离子光学器件接收从离子阱排出的离子并且冷却离子而不会明显碎裂。 离子分析仪接收从离子阱或离子光学器件喷出的离子，并根据离子的至少一种特性分离离子。

公开(公告)号：US09117644B2

公开(公告)日：2015-08-25

申请号：US14330373

申请日：2014-07-14

Applicant: Micromass UK Limited

Inventor： Martin Raymond Green ,  Jason Lee Wildgoose ,  Jeffery Mark Brown

IPC: H01J49/26 ,  H01J49/40 ,  H01J49/04 ,  H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0481 ,  H01J49/0072 ,  H01J49/065 ,  H01J49/40 ,  Y10T436/24

Abstract: A mass spectrometer is disclosed comprising an Electron Transfer Dissociation cell. Positive analyte ions are fragmented into fragment ions upon colliding with singly charged negative reagent ions with the cell. The cell comprises a plurality of ring electrodes which form a spherical trapping volume. Ions experience negligible RF heating over the majority of the trapping volume which enables the kinetic energy of the analyte and reagent ions to be reduced to just above thermal temperatures. An Electron Transfer Dissociation cell having an enhanced sensitivity is thereby provided. Fragment ions created within the cell may be cooled and may be transmitted onwardly to an orthogonal acceleration Time of Flight mass analyser enabling a significant improvement in the resolution of the mass analyser to be obtained.

Abstract translation: 公开了一种质谱仪，其包括电子转移解离单元。 当与单体电荷的负试剂离子碰撞时，阳性分析物离子被碎裂成碎片离子。 电池包括形成球形捕获体积的多个环形电极。 在大多数捕集体积下，离子经历可忽略的RF加热，使得分析物和试剂离子的动能能够降低到高于热温度。 从而提供具有增强的灵敏度的电子转移解离电池。 在细胞内产生的片段离子可以被冷却并且可以向内传播到正交加速时间飞行质量分析仪，使得可以获得质量分析仪的分辨率的显着提高。