公开(公告)号：US20220011327A1

公开(公告)日：2022-01-13

申请号：US17261811

申请日：2019-07-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Don W. Arnold ,  Thomas R. Covey ,  Chang Liu ,  Stanislaw Potyrala ,  Alex Tsipirovich

IPC: G01N35/00 ,  B03C1/01 ,  B03C1/28

Abstract: Electromagnetic systems and corresponding methods for assembling the electromagnetic systems are described. The electromagnetic systems can be used in fluid processing systems that include a plurality of fluid containers, each configured to define a fluid chamber that receives a fluid and a plurality of magnetic particles, and a plurality of electromagnets configured to generate a magnetic field within at least one of the plurality of the fluid containers. The fluid processing system can also include a PCB board that supplies the electromagnets with electrical current by establishing an electrical connection between electrical contact terminals included on the PCB board and spring loaded connections included on each electromagnet. A control component controls the electromagnetic field generated by each electromagnet to generate a plurality of magnetic field gradients within the at least one fluid container sufficient to magnetically influence the plurality of magnetic particles within the fluid in each fluid container.

公开(公告)号：US20210225631A1

公开(公告)日：2021-07-22

申请号：US17225622

申请日：2021-04-08

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Don W. Arnold ,  Thomas R. Covey ,  Chang Liu

IPC: H01J49/04 ,  G01N1/40 ,  H01J49/16

Abstract: Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which the flow of desorption solvent within a sampling probe fluidly coupled to an ion source can be selectively controlled such that one or more analyte species can be desorbed from a sample substrate inserted within the sampling probe within a decreased volume of desorption solvent for subsequently delivery to the ion source. In various aspects, sensitivity can be increased due to higher desorption efficiency (e.g., due to increased desorption time) and/or decreased dilution of the desorbed analytes. The methods and systems described herein can additionally or alternatively provide for the selective control of the flow rate of the desorption solvent within the sampling interface so as to enable additional processing steps to occur within the sampling probe (e.g., multiple samplings, reactions).

公开(公告)号：US10998179B2

公开(公告)日：2021-05-04

申请号：US16341718

申请日：2017-10-12

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Don W. Arnold ,  Thomas R. Covey ,  Chang Liu

IPC: H01J49/04 ,  G01N1/40 ,  H01J49/16 ,  G01N30/00

Abstract: Methods and systems for delivering a liquid sample to an ion source for the generation of ions and subsequent analysis by mass spectrometry are provided herein. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which the flow of desorption solvent within a sampling probe fluidly coupled to an ion source can be selectively controlled such that one or more analyte species can be desorbed from a sample substrate inserted within the sampling probe within a decreased volume of desorption solvent for subsequently delivery to the ion source. In various aspects, sensitivity can be increased due to higher desorption efficiency (e.g., due to increased desorption time) and/or decreased dilution of the desorbed analytes. The methods and systems described herein can additionally or alternatively provide for the selective control of the flow rate of the desorption solvent within the sampling interface so as to enable additional processing steps to occur within the sampling probe (e.g., multiple samplings, reactions).

公开(公告)号：US20210121905A1

公开(公告)日：2021-04-29

申请号：US16973801

申请日：2019-06-11

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Thomas R Covey ,  Chang Liu

IPC: B05B12/00 ,  G01F22/00 ,  H01J49/16 ,  H01J49/00 ,  B05B12/08

Abstract: Improvements in acoustically dispensed samples that are injected into an open port probe (OPP) are described. Apparatus and method are described that calibrate the volume dispensing determination and mechanisms in the acoustic dispenser to produce accurate and precise volumetric delivery.

公开(公告)号：US10770277B2

公开(公告)日：2020-09-08

申请号：US16198667

申请日：2018-11-21

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20190157060A1

公开(公告)日：2019-05-23

申请号：US16198667

申请日：2018-11-21

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Donald W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/00

CPC classification number: H01J49/0454 ,  B01L3/0268 ,  B01L2400/0439 ,  H01J49/0031 ,  H01J49/0404 ,  H01J49/165

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20180328934A1

公开(公告)日：2018-11-15

申请号：US15779944

申请日：2016-11-29

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: G01N33/58 ,  G01N27/62 ,  G01N33/68 ,  H01J49/00

CPC classification number: G01N33/582 ,  G01N27/624 ,  G01N33/58 ,  G01N33/6848 ,  H01J49/0045

Abstract: Methods and systems for separating and/or quantifying compounds, using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can provide for the quantification of one or more compounds, for example, using isomeric labels that can be less costly to produce relative to conventional tags that incorporate stable-isotope labels. The present teachings can quantify the relative amount of a compound based on the effect of using an easily charged functional group as well as a functional group positioned at a resonant or non-resonant position through a DMS. In some aspects, methods and systems in accordance with various aspects of the present teachings provide for the detection and/or quantification of the analytes labeled with isomeric tags that can be differentiated via a DMS upstream of a first stage mass analyzer and/or prior to fragmentation of the labeled analyte, for example.

公开(公告)号：US20170248547A1

公开(公告)日：2017-08-31

申请号：US15511712

申请日：2015-08-28

Applicant: DH Technologies Development PTE Ltd.

Inventor： John L Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: G01N27/62 ,  H01J49/10 ,  H01J49/04

CPC classification number: G01N27/624 ,  G01N33/6803 ,  H01J49/04 ,  H01J49/10

Abstract: Methods and systems for separating and/or quantifying peptides using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of one or more peptides, for example, peptides that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by complexing the peptides with a metal cation (e.g., Ca2+) prior to DMS. In some aspects, the present teachings can prevent proton stripping from ionized peptides that can occur in DMS to prevent unintended and/or undesirable alterations to the peptide's charge state distribution.