公开(公告)号：US11731133B2

公开(公告)日：2023-08-22

申请号：US17824759

申请日：2022-05-25

Applicant: Labcyte Inc.

Inventor： Richard N. Ellson

IPC: B01L3/00 ,  B01L3/02

CPC classification number: B01L3/50857 ,  B01L3/50855 ,  B01J2219/00313 ,  B01J2219/00319 ,  B01J2219/00362 ,  B01L3/0244 ,  B01L3/0268 ,  B01L2400/0436

Abstract: Provided herein is generally tubular container, preferably including a plurality of reservoirs defined therein. The container can be adapted for acoustic ejection of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs. Alternatively, the container can be adapted for extraction of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs using a non-acoustic liquid handling method.

公开(公告)号：US11691151B2

公开(公告)日：2023-07-04

申请号：US17521585

申请日：2021-11-08

Applicant: Labcyte Inc.

Inventor： Eric Sackmann ,  Sammy Datwani ,  Stephen Hinkson

IPC: B01L3/00 ,  B01L3/02 ,  G01N29/07 ,  G01N29/44 ,  G01N29/04 ,  G01N29/09 ,  G01N29/028

CPC classification number: B01L3/545 ,  B01L3/0268 ,  B01L3/56 ,  G01N29/028 ,  G01N29/043 ,  G01N29/07 ,  G01N29/09 ,  G01N29/4409 ,  B01J2219/00378 ,  B01L2300/021 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/0439 ,  G01N2291/011 ,  G01N2291/018 ,  G01N2291/0235 ,  G01N2291/101 ,  G01N2291/2695

Abstract: Embodiments of the present invention provide systems and methods for tagging and acoustically characterizing containers.

公开(公告)号：US11637006B2

公开(公告)日：2023-04-25

申请号：US17410758

申请日：2021-08-24

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: G01N29/02 ,  H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02 ,  G01N29/22

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20220088617A1

公开(公告)日：2022-03-24

申请号：US17424198

申请日：2020-01-31

Applicant: Labcyte Inc.

Inventor： Richard N. Ellson ,  Richard G. Stearns ,  Babur Hadimioglu

IPC: B05B1/08 ,  C12N13/00 ,  B05B17/06 ,  G01N15/10

Abstract: Systems and methods described herein employ focused acoustic energy applied to a reservoir containing a fluid to eject a fluid sample from the fluid sample reservoir, e.g. to an inlet of an analytical device. In many embodiments, the ejected fluid sample traverses an air gap separating the inlet of the analytical device from an upper surface of the fluid in the fluid sample reservoir. In many embodiments, the ejected fluid sample comprises one or more droplets ejected from the fluid sample reservoir, which can contain particles suspended in the fluid sample.

公开(公告)号：US11237086B2

公开(公告)日：2022-02-01

申请号：US17108931

申请日：2020-12-01

Applicant: Labcyte Inc.

Inventor： Joseph D. Olechno ,  Richard N. Ellson

IPC: G01N1/00 ,  G01N1/40 ,  B01D21/28 ,  B01L3/02 ,  B01L3/00 ,  C12N15/10 ,  G01N29/02 ,  H01J49/00 ,  H01J49/04 ,  G01N35/10

Abstract: A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.

公开(公告)号：US20220025382A1

公开(公告)日：2022-01-27

申请号：US17497792

申请日：2021-10-08

Applicant: Labcyte Inc.

Inventor： Jennifer M. Hardee ,  Richard N. Ellson ,  Richard G. Stearns ,  Babur Hadimioglu ,  Joseph D. Olechno ,  Marsha N. Blauwkamp

IPC: C12N15/64 ,  C12N15/87 ,  A61N7/00 ,  C12N9/22 ,  C12N13/00 ,  C12N15/11

Abstract: A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

公开(公告)号：US10981171B2

公开(公告)日：2021-04-20

申请号：US15783659

申请日：2017-10-13

Applicant: LABCYTE INC.

Inventor： Richard N. Ellson

IPC: B01L3/00 ,  B01L3/02

Abstract: Provided herein is generally tubular container, preferably including a plurality of reservoirs defined therein. The container can be adapted for acoustic ejection of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs. Alternatively, the container can be adapted for extraction of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs using a non-acoustic liquid handling method.

公开(公告)号：US10800170B1

公开(公告)日：2020-10-13

申请号：US16127849

申请日：2018-09-11

Applicant: Labcyte Inc.

Inventor： Richard G. Stearns ,  Mitchell W. Mutz ,  Richard N. Ellson

IPC: B41J2/045 ,  B41J2/14 ,  B05D5/00 ,  B05D1/02

Abstract: Devices and methods are provided for ejecting a droplet from a reservoir using focused acoustic radiation having a plurality of nonsimultaneous and discrete frequency ranges. Such frequency ranges may be used to control droplet volume and/or velocity. Optionally, satellite fluid ejection from the reservoir is suppressed.

公开(公告)号：US10770277B2

公开(公告)日：2020-09-08

申请号：US16198667

申请日：2018-11-21

Applicant: Labcyte, Inc. ,  DH Technologies Development Pte. Ltd.

Inventor： Sammy Datwani ,  Don W. Arnold ,  Lucien P. Ghislain ,  Chang Liu ,  Thomas Covey

IPC: H01J49/04 ,  H01J49/16 ,  H01J49/00 ,  B01L3/02

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.

公开(公告)号：US20190157061A1

公开(公告)日：2019-05-23

申请号：US16198759

申请日：2018-11-21

Applicant: Labcyte, Inc.

Inventor： Sammy Datwani ,  Lucien P. Ghislain

IPC: H01J49/04 ,  H01J49/00 ,  H01J49/16

Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g., an electrospray ion source, prior to entering the mass spectrometer or other analytical instrument. The method employs active flow control and enables real-time kinetic measurements.