公开(公告)号：US08102523B1

公开(公告)日：2012-01-24

申请号：US13239632

申请日：2011-09-22

Applicant: Charles P. Marsh ,  Eric Olson ,  Todor I. Donchev ,  Ivan Petrov ,  Jianguo Wen ,  Ryan Franks ,  Dongxiang Liao

Inventor： Charles P. Marsh ,  Eric Olson ,  Todor I. Donchev ,  Ivan Petrov ,  Jianguo Wen ,  Ryan Franks ,  Dongxiang Liao

IPC: G01N1/10

CPC classification number: H01J37/26 ,  G02B21/34 ,  H01J37/20 ,  H01J2237/2004 ,  H01J2237/2005 ,  H01J2237/28 ,  Y10T29/49826

Abstract: A method for using a reusable sample-holding device for readily loading very small wet samples for observation of the samples by microscopic equipment, in particular in a vacuum environment. The method may be used with a scanning electron microscope (SEM), a transmission electron microscope (TEM), an X-ray microscope, optical microscope, and the like. For observation of the sample, the method provides a thin-membrane window etched in the center of each of two silicon wafers abutting to contain the sample in a small uniform gap formed between the windows. This gap may be adjusted by employing spacers. Alternatively, the thickness of a film established by the fluid in which the sample is incorporated determines the gap without need of a spacer. To optimize resolution each window may have a thickness on the order of 50 nm and the gap may be on the order of 50 nm.

Abstract translation: 一种使用可重复使用的样品保持装置的方法，用于容易地加载非常小的湿样品，以通过微观设备特别是在真空环境中观察样品。 该方法可以用扫描电子显微镜（SEM），透射电子显微镜（TEM），X射线显微镜，光学显微镜等来使用。 为了观察样品，该方法提供了在两个硅晶片的每一个的中心蚀刻的薄膜窗口，其邻接以将样品容纳在窗口之间形成的小的均匀间隙中。 该间隙可以通过使用间隔物来调节。 或者，通过其中包含样品的流体建立的膜的厚度确定了间隙而不需要间隔物。 为了优化分辨率，每个窗口可以具有大约50nm的厚度，并且间隙可以在50nm量级。

公开(公告)号：US20110284745A1

公开(公告)日：2011-11-24

申请号：US13099544

申请日：2011-05-03

Applicant: Hidetoshi Nishiyama ,  Nobuo Saotome ,  Mitsuo Suga

Inventor： Hidetoshi Nishiyama ,  Nobuo Saotome ,  Mitsuo Suga

IPC: G01N23/22 ,  G01N23/00 ,  G21K5/08

CPC classification number: H01J37/20 ,  G01N23/2204 ,  H01J2237/2003 ,  H01J2237/2004

Abstract: A sample holder, inspection apparatus, and an inspection method using the sample holder having a film including a first surface and a second surface. A liquid sample may be held on the first surface. The film is made of two or more layers. A primary beam irradiation device is installed in a reduced-pressure space. Consequently, the sample can be observed or inspected while maintaining the sample at the atmospheric pressure.

Abstract translation: 使用具有包括第一表面和第二表面的膜的样品保持器的样品保持器，检查装置和检查方法。 液体样品可以保持在第一表面上。 该片由两层或多层制成。 主光束照射装置安装在减压空间中。 因此，可以在将样品保持在大气压力的同时观察或检查样品。

公开(公告)号：US07659510B2

公开(公告)日：2010-02-09

申请号：US12078223

申请日：2008-03-28

Applicant: Chih-Yu Chao

Inventor： Chih-Yu Chao

IPC: H01J49/44

CPC classification number: H01J37/20 ,  G01N1/42 ,  H01J2237/2001 ,  H01J2237/2002 ,  H01J2237/2003 ,  H01J2237/2004 ,  H01J2237/26

Abstract: The present invention relates to a cryo-charging specimen holder for the electron microscope, particularly to a cryo-charging specimen holder for the electron microscope to hold various biological materials. The major feature of the invention is to charge the biological specimen and freeze the specimen at low temperature. The ice around the biological sample is also doped, so that after charging the doped ice surrounding the sample has a conductivity level comparable to that of conductor. Therefore, the sample can be embedded by the doped and charged ice obtaining the property of conductor, in order to be observed by the electron microscope.

Abstract translation: 本发明涉及一种用于电子显微镜的低温充电试样架，特别涉及用于电子显微镜保持各种生物材料的低温充电试样架。 本发明的主要特征是对生物样品充电并在低温下冷冻试样。 生物样品周围的冰也被掺杂，因此在充电之后，样品周围的掺杂冰具有与导体相当的导电率。 因此，可以通过掺杂和充电的冰来嵌入样品，获得导体的性质，以便通过电子显微镜观察。

公开(公告)号：US20090166536A1

公开(公告)日：2009-07-02

申请号：US12342897

申请日：2008-12-23

Applicant: Mitsuo Suga ,  Hidetoshi Nishiyama

Inventor： Mitsuo Suga ,  Hidetoshi Nishiyama

IPC: G01N23/02 ,  G01N21/01

CPC classification number: H01J37/20 ,  H01J37/26 ,  H01J2237/2004 ,  H01J2237/2005 ,  H01J2237/201 ,  H01J2237/204 ,  H01J2237/206 ,  H01J2237/262 ,  H01J2237/28

Abstract: A sample holder used in SEM (scanning electron microscopy) or TEM (transmission electron microscopy) permitting observation and inspection at higher resolution. The holder has a frame-like member provided with an opening that is covered with a film. The film has a first surface on which a sample is held. The thickness D of the film and the length L of the portion of the film providing a cover over the opening in the frame-like member satisfy a relationship given by L/D

Abstract translation: 用于SEM（扫描电子显微镜）或TEM（透射电子显微镜）中的样品架，允许以更高分辨率观察和检查。 保持器具有设置有被膜覆盖的开口的框架状构件。 该膜具有保持样品的第一表面。 膜的厚度D和在框状构件的开口上设置盖的膜的部分的长度L满足由L / D <200,000给出的关系。

公开(公告)号：US20090045349A1

公开(公告)日：2009-02-19

申请号：US11660929

申请日：2005-08-25

Applicant: David Sprinzak ,  Yiftah Karni ,  Yitzhak Ronen

Inventor： David Sprinzak ,  Yiftah Karni ,  Yitzhak Ronen

IPC: G01N21/51 ,  G01K5/10

CPC classification number: H01J37/20 ,  G01N23/2204 ,  H01J2237/2004 ,  H01J2237/2006 ,  H01J2237/2007 ,  H01J2237/201

Abstract: A sample container assembly for use in a microscope including a sample enclosure, an electron beam permeable, fluid impermeable, membrane sealing the sample enclosure from a volume outside the sample enclosure and a pressure controller assembly communicating between the sample enclosure and a volume outside the sample enclosure.

Abstract translation: 用于显微镜的样品容器组件，包括样品外壳，电子束可透过的流体不可渗透的膜，将样品外壳与样品外壳外的体积密封;以及在样品外壳和样品外部的体积之间连通的压力控制器组件 外壳。

公开(公告)号：US07476871B2

公开(公告)日：2009-01-13

申请号：US11452333

申请日：2006-06-14

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K5/10 ,  G21K5/08 ,  G01N23/04

CPC classification number: H01J37/20 ,  H01J2237/188 ,  H01J2237/2004 ,  H01J2237/262

Abstract: A specimen box for an electron microscope capable of observing a general specimen or a live cell is formed of a housing. The housing includes a receiving chamber formed therein and at least one view hole formed on each of a top side thereof and a bottom side thereof and communicating with the receiving chamber and coaxially aligned with the other. The distance between a bottom end of the view hole located on the top side of the housing and a top end of the view hole located on the bottom side of the housing is smaller than 50 μm. The housing is ultra-thin to enable penetration of the electron beam therethrough even though a liquid specimen is injected into the housing. A general specimen or a live cell can be put into the housing for the microscopic observation under the electron microscope.

Abstract translation: 用于观察一般样品或活细胞的电子显微镜的标本盒由壳体形成。 壳体包括形成在其中的接收室和形成在其顶侧和底侧中的每一个上的至少一个视孔，并与接收室连通并与另一个同轴对准。 位于壳体顶部的视野孔的底端与位于壳体底侧的视野孔的顶端之间的距离小于50μm。 壳体是超薄的，以使得即使将液体样本注入到壳体中也能够穿过电子束。 一般标本或活细胞可以放入外壳中进行显微镜观察。

公开(公告)号：US07388211B2

公开(公告)日：2008-06-17

申请号：US11453810

申请日：2006-06-16

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: H01J37/18 ,  H01J37/20

CPC classification number: H01J37/20 ,  H01J2237/188 ,  H01J2237/2004 ,  H01J2237/262

Abstract: A semi-closed observational environment for an electron microscope includes a housing having at least two spacers for partitioning itself into a receiving chamber, a gas chamber below the receiving chamber, a buffer chamber below the gas chamber, at least one gas inlet formed at the gas chamber, and at least one pumping port formed at the buffer chamber. The receiving chamber has at least two view holes formed at top and bottom sides thereof respectively, wherein the latter communicates with the gas chamber. The gas chamber has at least one gas aperture formed at a bottom side thereof for communication with the buffer chamber. The buffer chamber has at least one outer aperture formed at a bottom side thereof for communication with outside. The two view holes of the receiving chamber are coaxially aligned with the gas and outer apertures. A film is mounted to and seals the upper view hole.

Abstract translation: 用于电子显微镜的半封闭观察环境包括壳体，其具有至少两个用于将其自身分配到接收室中的间隔件，在接收室下方的气室，在气室下方的缓冲室，形成在该腔室处的至少一个气体入口 气室和形成在缓冲室处的至少一个泵送口。 接收室具有分别在其顶侧和底侧形成的至少两个视孔，其中后视图与气室连通。 气室具有形成在其底侧的至少一个气孔，用于与缓冲室连通。 缓冲室具有形成在其底侧的至少一个外孔，用于与外部连通。 接收室的两个视野孔与气体和外部孔同轴对准。 胶片被安装到并密封上视图孔。

公开(公告)号：US20070145288A1

公开(公告)日：2007-06-28

申请号：US11453810

申请日：2006-06-16

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G21K5/10

CPC classification number: H01J37/20 ,  H01J2237/188 ,  H01J2237/2004 ,  H01J2237/262

Abstract: A semi-closed observational environment for an electron microscope includes a housing having at least two spacers for partitioning itself into a receiving chamber, a gas chamber below the receiving chamber, a buffer chamber below the gas chamber, at least one gas inlet formed at the gas chamber, and at least one pumping port formed at the buffer chamber. The receiving chamber has at least two view holes formed at top and bottom sides thereof respectively, wherein the latter communicates with the gas chamber. The gas chamber has at least one gas aperture formed at a bottom side thereof for communication with the buffer chamber. The buffer chamber has at least one outer aperture formed at a bottom side thereof for communication with outside. The two view holes of the receiving chamber are coaxially aligned with the gas and outer apertures. A film is mounted to and seals the upper view hole.

Abstract translation: 用于电子显微镜的半封闭观察环境包括壳体，其具有至少两个用于将其自身分配到接收室中的间隔件，在接收室下方的气室，在气室下方的缓冲室，形成在该腔室的至少一个气体入口 气室和形成在缓冲室处的至少一个泵送口。 接收室具有分别在其顶侧和底侧形成的至少两个视孔，其中后视图与气室连通。 气室具有形成在其底侧的至少一个气孔，用于与缓冲室连通。 缓冲室具有形成在其底侧的至少一个外孔，用于与外部连通。 接收室的两个视野孔与气体和外部孔同轴对准。 胶片被安装到并密封上视图孔。

公开(公告)号：US07220971B1

公开(公告)日：2007-05-22

申请号：US11320515

申请日：2005-12-28

Applicant: Sha X. Chang ,  Otto Z. Zhou

Inventor： Sha X. Chang ,  Otto Z. Zhou

IPC: G01K1/08

CPC classification number: H01J37/304 ,  G21K5/04 ,  H01J33/02 ,  H01J33/04 ,  H01J37/302 ,  H01J37/317 ,  H01J2201/30469 ,  H01J2237/0635 ,  H01J2237/2004 ,  H01J2237/2006 ,  H01J2237/316

Abstract: Multi-pixel electron microbeam irradiator systems and methods are provided with particular applicability for selectively irradiating predetermined cells or cell locations. A multi-pixel electron microbeam irradiator system can include a plurality of individually addressable electron field emitters sealed in a vacuum. The multi-pixel electron microbeam irradiator system can include an anode comprising one or more electron permeable portions corresponding to the plurality of electron field emitters. Further, the multi-pixel electron microbeam irradiator system can include a controller operable to individually control electron extraction from each of the electron field emitters for selectively irradiating predetermined locations such as cells or cell locations.

Abstract translation: 提供了多像素电子微束照射器系统和方法，其特别适用于选择性地照射预定的细胞或细胞位置。 多像素电子微束照射器系统可以包括在真空中密封的多个可单独寻址的电子场发射器。 多像素电子微束照射器系统可以包括阳极，该阳极包括对应于多个电子场发射体的一个或多个电子透过部分。 此外，多像素电子微束照射器系统可以包括控制器，其可操作以单独地控制来自每个电子场发射器的电子提取，以选择性地照射诸如单元或单元位置的预定位置。

公开(公告)号：US20070090289A1

公开(公告)日：2007-04-26

申请号：US11370916

申请日：2006-03-09

Applicant: Chih-Yu Chao ,  Wen-Jiunn Hsieh

Inventor： Chih-Yu Chao ,  Wen-Jiunn Hsieh

IPC: G01N23/04

CPC classification number: G01N23/04 ,  G01N2223/6126 ,  H01J2237/2004

Abstract: A method of observing a live unit under an electron microscope includes the steps of (A) preparing a live environment inside the electron microscope, wherein the live environment is provided with at least one live unit and a predetermined environmental condition keeping basic physiology of the live unit functional, at least one pair of view windows is located opposite to each other, and the live unit includes at least two objects that can bear different critical charge densities respectively; and (B) irradiating the live unit with a particle beam of predetermined intensity through the view windows, and then display the live unit on an imaging device of the electron microscope, wherein the product of the predetermined intensity of the particle beam and the predetermined duration equals the predetermined charge density that is smaller than or equal to the critical charge density of the object of the live unit.

Abstract translation: 在电子显微镜下观察活体单元的方法包括以下步骤：（A）准备电子显微镜内的活体环境，其中活体环境设置有至少一个活体单元和保持活体基本生理学的预定环境条件 单元功能，至少一对视窗彼此相对定位，并且活动单元包括分别可承受不同关键电荷密度的至少两个物体; 和（B）通过视窗用预定强度的粒子束照射活体单元，然后将活体单元显示在电子显微镜的成像装置上，其中粒子束的预定强度与预定持续时间的乘积 等于小于或等于活动单元物体的临界电荷密度的预定电荷密度。