公开(公告)号：US20200046768A1

公开(公告)日：2020-02-13

申请号：US16536948

申请日：2019-08-09

Applicant: University Health Network

Inventor： Li Zhang ,  Dalam Ly ,  Linan Fang

IPC: A61K35/17 ,  C07K16/28 ,  A61P35/00

Abstract: Methods for the treatment of cancer using double negative T cells (DNTs) and a PD-1 or PD-L1 inhibitor are described. Tumors treated with DNTs and a PD-1 inhibitor exhibited increased DNT cell infiltration and increased cytotoxicity towards non-small cell lung cancer (NSCLC) cells. Also described are compositions and kits comprising DNTs and a PD-1 or PD-L1 inhibitor and their use for the treatment of cancer.

公开(公告)号：US20230011889A1

公开(公告)日：2023-01-12

申请号：US17782071

申请日：2020-12-07

Applicant: University Health Network

Inventor： Li Zhang ,  Jong Bok Lee ,  Daniel Vasic ,  Ismat Khatri ,  Dalam Ly ,  Yuki Sze Long Leung

IPC: A61K35/17 ,  C07K14/725 ,  C12N5/0783 ,  A61P35/00

Abstract: The disclosure relates to the development and use of CD4− CD8− double negative T (DNT) cells genetically modified to bind to one or more target antigens to enhance DNT cell anti-cancer activity such as with a chimeric antigen receptor (CAR). Genetically modified DNT cells can be generated ex vivo and expanded from allogeneic healthy donor cells and used as off-the-shelf therapy to overcome allogeneic graft-versus-host disease (GvHD) and/or host-versus-graft rejection in the treatment of cancer.

公开(公告)号：US11534460B2

公开(公告)日：2022-12-27

申请号：US15573379

申请日：2016-05-11

Applicant: UNIVERSITY HEALTH NETWORK

Inventor： Li Zhang ,  Paulina Achita ,  Jong Lee ,  Dalam Ly ,  Dzana Dervovic

IPC: A61K35/17 ,  C12N5/0783 ,  A61P35/00

Abstract: There is provided herein a method for expanding human CD4-CD8- regulatory T cells (DN Tregs) from a population of cells comprising DN Tregs, comprising: culturing the population of cells with artificial antigen presenting cells (APCs), preferably the DN Tregs are αβ-TCR-CD56- or alternatively γ8-TCR+.