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    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    1.
    发明申请
    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    光学系统和生物分析方法

    公开(公告)号：WO2013049709A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/058107

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence ,  CHENG, Francis

    IPC: G01N21/64

    CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

    Abstract: An instrument (1000) for processing and/or measuring a biological process contains an excitation source (110), a sample holder (204), an optical sensor (118), an excitation optical system (120), and an emission optical system (125). The sample holder (204) is configured to receive a plurality of biological samples. The optical sensor (118) is configured to receive an emission from the biological samples. The excitation optical system (120) is disposed along an excitation optical path (126) and is configured to direct the electromagnetic radiation from the excitation source (110) to the biological samples. The emission optical system (125) is disposed along an emission optical path (128) and is configured to direct electromagnetic emissions from the biological samples to the optical sensor (118). The instrument further contains a plurality of filter assemblies (130, 132) configured to be interchangeably located along at least one of the optical paths. The plurality of filter components (131) includes a first filter component (138) characterized by a first optical power and a first filter (140) having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly (142) is characterized by a second optical power and a second filter (145) having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength. The second optical power differs from the first optical power by an amount sufficient to at least partially compensate for an aberration introduced by the second filter (145) relative to the first filter (140).

    Abstract translation: 用于处理和/或测量生物过程的仪器（1000）包括激发源（110），样品保持器（204），光学传感器（118），激发光学系统（120）和发射光学系统 125）。 样品架（204）被配置为接收多个生物样品。 光学传感器（118）被配置为接收来自生物样品的发射。 激发光学系统（120）沿着激励光路（126）设置并且被配置为将来自激发源（110）的电磁辐射引导到生物样品。 发射光学系统（125）沿着发射光路（128）设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器（118）。 该仪器还包括多个过滤器组件（130,132），其被配置为沿着至少一个光路可互换地定位。 多个滤波器组件（131）包括具有第一光功率的第一滤波器组件（138）和具有第一滤波器功能的第一滤波器（140），第一滤波器功能的特征在于第一低通 波长或第一高通波长。 第二滤波器组件（142）的特征在于具有第二滤波器功能的第二光功率和第二滤波器（145），第二滤波器功能包括不同于第一低通滤波器的第二低通波长中的至少一个， 通过波长或与第一高通波长不同的第二高通波长。 第二光功率与第一光功率不同，足以至少部分地补偿由第二滤光器（145）相对于第一滤光器（140）引入的像差。

    METHOD AND SYSTEMS FOR IMAGE ANALYSIS IDENTIFICATION
    2.
    发明申请
    METHOD AND SYSTEMS FOR IMAGE ANALYSIS IDENTIFICATION 审中-公开
    用于图像分析识别的方法和系统

    公开(公告)号：WO2013049442A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/057714

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang Terence ,  CHENG, Francis T.

    IPC: G01N35/00

    CPC classification number: G01N35/00732 ,  G01N35/028 ,  G01N2015/0053 ,  G01N2035/00752 ,  G06T7/0012 ,  G06T2207/10004 ,  G06T2207/30004

    Abstract: A computer-implemented method for identifying a first object-of-interest is provided. The first object-of-interest includes two identifiers and a sample portion. The method includes imaging the first object-of-interest including the two identifiers. The imaging generates a first set of image data. The method further includes determining a position of the first object-of- interest in the field-of-view of an optical sensor and determining the two identifiers from the first set of image data. The method includes identifying the first object-of-interest based on the two identifiers.

    Abstract translation: 提供了一种用于识别第一感兴趣物体的计算机实现的方法。 第一个感兴趣的对象包括两个标识符和一个样本部分。 该方法包括对第一感兴趣物体成像，包括两个标识符。 成像产生第一组图像数据。 该方法还包括确定在光学传感器的视场中的第一感兴趣对象的位置，并从第一组图像数据确定两个标识符。 该方法包括基于两个标识符识别第一感兴趣对象。

    METHODS AND SYSTEMS FOR VISUALIZING AND EVALUATING DATA
    4.
    发明申请
    METHODS AND SYSTEMS FOR VISUALIZING AND EVALUATING DATA 审中-公开
    用于可视化和评估数据的方法和系统

    公开(公告)号：WO2013049443A1

    公开(公告)日：2013-04-04

    申请号：PCT/US2012/057716

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JANAWAY, Gordon ,  ALIMINATI, Manjula ,  WU, Ruoyun ,  FORTESCUE, David ,  SHEN, Ming

    IPC: C12Q1/68

    CPC classification number: G06F19/24 ,  C12Q1/6851 ,  C12Q2537/165 ,  C12Q2563/159

    Abstract: A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.

    Abstract translation: 提供了产生数字聚合酶链反应（dPCR）结果的计算机实现的方法。 该方法包括在放大期间的第一时间，从多个样本中检测出第一组发射数据，每个采样包括多个采样区域的样本区域。 该方法还包括部分地基于第一组发射数据确定多个样本中的每个样本的正或负放大确定。 基于多个样本的正或负放大确定产生dPCR结果。

    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    5.
    发明公开
    OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    OPTISCHE SYSTEME UND VERFAHREN ZUR BIOLOGISCHEN ANALYZE

    公开(公告)号：EP2761277A1

    公开(公告)日：2014-08-06

    申请号：EP12783710.2

    申请日：2012-09-28

    Applicant: Life Technologies Corporation

    Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence

    IPC: G01N21/64

    CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

    Abstract: An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. The optical sensor is configured to receive an emission from the biological samples. The excitation optical system is disposed along an excitation optical path and is configured to direct the electromagnetic radiation from the excitation source to the biological samples. The emission optical system is disposed along an emission optical path and is configured to direct electromagnetic emissions from the biological samples to the optical sensor. The instrument further contains a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength.

    Abstract translation: 用于处理和/或测量生物过程的仪器包含激发源，样品架，光学传感器，激发光学系统和发射光学系统。 样品架配置成接收多个生物样品。 光学传感器被配置为接收来自生物样品的发射。 激发光学系统沿着激发光学路径设置并且被配置为将来自激发源的电磁辐射引导到生物样品。 发射光学系统沿着发射光路设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器。 仪器还包括多个过滤器组件，其被配置为沿着至少一个光路可互换地定位。 多个滤波器部件包括第一滤波器组件，其特征在于第一光功率和具有第一滤波器功能的第一滤波器，第一滤波器功能的特征在于第一低通波长或第一高通波长中的至少一个。 第二滤波器组件的特征在于具有第二滤波器功能的第二光功率和第二滤波器，第二滤波器功能包括与第一低通波长不同的第二低通波长或第二高通波长中的至少一个 通过波长不同于第一高通波长。

    METHODS AND SYSTEMS FOR VISUALIZING AND EVALUATING DATA
    8.
    发明公开
    METHODS AND SYSTEMS FOR VISUALIZING AND EVALUATING DATA 有权
    VERFAHREN UND SYSTEME ZUR VISUALISIERUNG UND AUSWERTUNG VON DATEN

    公开(公告)号：EP2761027A1

    公开(公告)日：2014-08-06

    申请号：EP12784368.8

    申请日：2012-09-28

    Applicant: Life Technologies Corporation

    Inventor： JANAWAY, Gordon ,  ALIMINATI, Manjula ,  WU, Ruoyun ,  FORTESCUE, David ,  SHEN, Ming

    IPC: C12Q1/68

    CPC classification number: G06F19/24 ,  C12Q1/6851 ,  C12Q2537/165 ,  C12Q2563/159

    Abstract: A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.

    Abstract translation: 提供了一种计算机实现的产生数字聚合酶链反应（dPCR）结果的方法。 该方法包括在放大期间的第一时间，从多个样本中检测出第一组发射数据，每个样本包括多个采样区域的采样区域。 该方法还包括部分地基于第一组发射数据来确定多个样本中的每个样本的正或负放大确定。 基于多个样本的正或负放大确定产生dPCR结果。

    METHOD AND SYSTEMS FOR IMAGE ANALYSIS IDENTIFICATION
    9.
    发明公开
    METHOD AND SYSTEMS FOR IMAGE ANALYSIS IDENTIFICATION 有权
    VERFAHREN UND SYSTEME ZUR BILDANALYSE UNDIDIDICATION

    公开(公告)号：EP2761302A1

    公开(公告)日：2014-08-06

    申请号：EP12781206.3

    申请日：2012-09-28

    Applicant: Life Technologies Corporation

    Inventor： FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang Terence ,  CHENG, Francis T.

    IPC: G01N35/00

    CPC classification number: G01N35/00732 ,  G01N35/028 ,  G01N2015/0053 ,  G01N2035/00752 ,  G06T7/0012 ,  G06T2207/10004 ,  G06T2207/30004

    Abstract: A computer-implemented method for identifying a first object-of-interest is provided. The first object-of-interest includes two identifiers and a sample portion. The method includes imaging the first object-of-interest including the two identifiers. The imaging generates a first set of image data. The method further includes determining a position of the first object-of- interest in the field-of-view of an optical sensor and determining the two identifiers from the first set of image data. The method includes identifying the first object-of-interest based on the two identifiers.

    Abstract translation: 提供了一种用于识别第一感兴趣物体的计算机实现的方法。 第一个感兴趣的对象包括两个标识符和一个样本部分。 该方法包括对第一感兴趣物体成像，包括两个标识符。 成像产生第一组图像数据。 该方法还包括确定第一感兴趣物体在光学传感器的视场中的位置，并根据第一组图像数据确定两个标识符。 该方法包括基于两个标识符识别第一感兴趣对象。

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