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    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    102.
    发明专利
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 未知

    公开(公告)号：SG11201407901PA

    公开(公告)日：2015-01-29

    申请号：SG11201407901P

    申请日：2013-05-21

    Applicant: FLUIDIGM CORP

    Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    IPC: C12Q1/68

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    MICROFLUIDIC DEVICES AND METHODS OF USING SAME
    103.
    发明专利
    MICROFLUIDIC DEVICES AND METHODS OF USING SAME 未知

    公开(公告)号：CA2807591C

    公开(公告)日：2014-11-18

    申请号：CA2807591

    申请日：2004-04-05

    Applicant: FLUIDIGM CORP

    Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

    IPC: C12M1/40 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/38 ,  C12P19/34 ,  C12Q1/68 ,  G01N1/00

    Abstract: Introducing a sample solution that includes a target nucleic acid polymer into a microfluidic device that has a sample region with: (i) a flow channel in fluid communication with one fluid inlet and one fluid outlet; and (ii) a plurality of valves that, when closed, partition the flow channel into isolated reaction sites. Each valve is formed from an elastomeric membrane that separates a control channel from the flow channel and which is disposed to be deflected into or retracted out from the flow channel in response to an actuation force to the control channel, thereby forming the valve. The method includes closing the valves, thereby partitioning the flow channel into isolated reaction sites containing portions of said sample solution; exposing the reaction sites to conditions under which the nucleic acid polymer is amplified to produce an amplification product; and opening said valves and displacing sample solution out of the flow channel.

    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    107.
    发明专利
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 未知

    公开(公告)号：SG10201402770YA

    公开(公告)日：2014-08-28

    申请号：SG10201402770Y

    申请日：2010-04-02

    Applicant: FLUIDIGM CORP

    Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

    Abstract: The present invention provides an elastomer microfluidic device that enables simultaneous amplification of multiple nucleic acid samples with multiple pairs of primers in a matrix format as well as recovery of the products e.g. for next generation sequencing. The flow of samples and reagents as well as the compartmentalization of individual reactions in the multiple channels and chambers is controlled using valves and dilation pumping / volumetric capacitive pumping.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    109.
    发明专利
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 未知

    公开(公告)号：CA2878787A1

    公开(公告)日：2013-09-06

    申请号：CA2878787

    申请日：2013-02-28

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN ,  KIMBALL JAKE ,  MAUNG MYO THU ,  MAY ANDREW ,  NORRIS MICHAEL C ,  TOPPANI DOMINIQUE G ,  UNGER MARC A ,  WANG JING ,  WEST JASON A A

    IPC: G01N15/10

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    ОПРЕДЕЛЕНИЕ ВАРИАЦИЙ КОЛИЧЕСТВА КОПИЙ, СПОСОБЫ И СИСТЕМЫ
    110.
    发明专利
    ОПРЕДЕЛЕНИЕ ВАРИАЦИЙ КОЛИЧЕСТВА КОПИЙ, СПОСОБЫ И СИСТЕМЫ 未知

    公开(公告)号：EA018555B1

    公开(公告)日：2013-08-30

    申请号：EA201070349

    申请日：2008-09-08

    Applicant: FLUIDIGM CORP

    Inventor： RAMAKRISHNAN RAMESH

    IPC: G01N33/48

    Abstract: Изобретениеотноситсяк способами системамдляопределениявариацииколичествакопийполинуклеотида-мишенив геномеиндивидуума, включающимоснованныенаамплификацииспособы. Способымогутвключатьпреамплификациюобразцас последующимраспределениемобразцавомножествореакционныхобъемов, количественноеопределениеполинуклеотида-мишении эталонногополинуклеотидаи анализдляопределенияотносительногоколичествакопийполинуклеотиднойпоследовательности-мишенив геномеиндивидуума.

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