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公开(公告)号：KR1020090093292A
公开(公告)日：2009-09-02
申请号：KR1020080018738
申请日：2008-02-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
Abstract translation: 提供雪花酵母的溶菌提取物以增加嗜中性粒细胞的活化并增强针对致病菌的非特异性防御活性。 免疫增强剂，疫苗佐剂，佐剂处理剂或饲料添加剂包括作为活性成分的雪花酵母的溶菌提取物。 酿酒酵母的溶菌提取物的制备方法包括：在培养基中在26℃下培养雪花酵母（Saccharomyces chevalieri）72小时的步骤; 离心培养基并悬浮于PBS中的步骤; 加入溶解剂并在室温下处理2-3天的步骤; 用显微镜确认溶出状态的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在冰箱中干燥和保持的步骤。
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公开(公告)号：KR1020090091884A
公开(公告)日：2009-08-31
申请号：KR1020080017089
申请日：2008-02-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
Abstract translation: 提供含有接合酵母发酵酵母裂解物和辅助治疗剂的动物免疫增强剂和疫苗佐剂添加剂，以增强中性粒细胞的活化和非特异性保护作用来攻击接种。 免疫增强剂，疫苗佐剂添加剂，辅助治疗剂和饲料添加剂包括接合酵母（Zygosaccharomyces bailii lysates）。 一种制造接合酵母发酵酵母裂解物的方法，包括：在培养基中培养接合酵母属酵母在26℃72小时的步骤; 将培养基离心并悬浮于磷酸盐缓冲盐水（PBS）中的步骤; 在室温下加入溶解剂并搅拌2〜3天的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在恒温室中干燥和保持的步骤。
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公开(公告)号：KR1020090039230A
公开(公告)日：2009-04-22
申请号：KR1020070104736
申请日：2007-10-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/00
Abstract: A new vaccine to duck hepatitis virus is provided to have excellence in resistance against epidemic duck hepatitis and lessen damage caused by the duck hepatitis. A method for producing vaccine(deposition No. KCCM10883P) of duck hepatitis virus having sequence of sequence No. 1 [SEQ ID NO:1] comprises: a step of diluting recently epidemic duck hepatitis virus in phosphate buffer; a step of inoculating 7-9 days old chicken embryo; a step of culturing the inoculated chicken embryo at 37°C for three days; and a step of collecting the cultured chicken embryo and performing subculture sequencially to adapt the chicken embryo. A process for manufacturing a new vaccine is performed with: the step of attenuating the duck hepatitis virus; a step of testing restoration of attenuated virus; a step of searching defense ability of vaccine to selected duck hepatitis virus; a step of searching defense ability in each pathway; a step of searching minimum amount of the vaccine; and a step of safety and amount of the vaccine.
Abstract translation: 提供了一种新型的鸭肝炎病毒疫苗，具有卓越的抗流行性鸭肝炎防治能力，减轻了鸭肝炎所造成的伤害。 具有序列号1 [SEQ ID NO：1]序列的鸭肝炎病毒疫苗（沉积编号KCCM10883P）的制备方法包括：在磷酸盐缓冲液中稀释近期流行的鸭肝炎病毒的步骤; 接种7-9天龄鸡胚的步骤; 将接种的鸡胚在37℃培养3天的步骤; 并收集培养的鸡胚并顺序进行亚文化以适应鸡胚的步骤。 制备新疫苗的方法是：减毒鸭肝炎病毒的步骤; 测试减毒病毒恢复的一个步骤; 检测疫苗对选择的鸭肝炎病毒的防御能力的一个步骤; 在每个途径中寻找防御能力的一个步骤; 搜索最少量的疫苗的步骤; 和疫苗的安全和数量的步骤。
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公开(公告)号：KR100890463B1
公开(公告)日：2009-03-26
申请号：KR1020070094861
申请日：2007-09-18
Applicant: 성균관대학교산학협력단 , (주)인비트로플랜트 , 아주대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Inventor： 이석찬 , 김용성 , 권명희 , 김태현 , 김정선 , 이건섭 , 심혜경 , 박을용 , 정유철 , 김기윤 , 정의정 , 이우람 , 김영림 , 손종남 , 이승현 , 송재영 , 최은진
CPC classification number: C07K16/10 , A61K39/00 , A61K39/395 , A61K2039/505 , A61K2300/00 , C07K14/47 , C07K16/44 , C07K2317/622 , C07K2317/76 , C07K2317/92
Abstract: 본 발명은 동물 세포에 침입하는 외래 유래의 핵산 사슬에 대해서 결합능과 그 분해능을 동시에 보유하나 동물 세포 자신에 대해서는 세포독성이 없는 단백질 또는 그 단백질을 암호화하는 핵산 서열을 유효성분으로 함유하는 동물 세포 감염 바이러스에 대한 항바이러스제 및, 그 단백질 또는 그 단백질을 암호화하는 핵산 서열을 함유하는 항바이러스 동물 세포을 제공하는데,
외래 침입의 핵산 사슬에 대해서만 선택적으로 분해능이 있고, 동물 세포 자신에 대해서는 세포독성이 없어 동물 세포 자체를 사멸시키지 않는 뛰어난 효과를 발휘한다.
결합능, 단백질, 바이러스, 분해능, 항바이러스제, 핵산-
145.发明公开동물의 면역능을 증강시키는 노란 민들레 열탕 추출물, 그제법 및 용도 无效用于改善动物免疫力的UM UM AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME AME
公开(公告)号：KR1020090021611A
公开(公告)日：2009-03-04
申请号：KR1020070086251
申请日：2007-08-27
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/288 , A61P37/02
Abstract: Provided is an immunostimulant using dandelion extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunostimulant using dandelion extract comprises dandelion extract obtained by a hot water process as an active ingredient. A method for preparing the immunostimulant comprises a steps of extracting the dandelion extract at a temperature of 90~100°C by hot distilled water. The dandelion extract can be used independently or with additives which are generally used.
Abstract translation: 提供使用蒲公英提取物的免疫刺激剂，其改善对病原体的非特异性防御的功能并增加动物免疫细胞如嗜中性粒细胞和巨噬细胞的活性。 使用蒲公英提取物的免疫刺激剂包含通过热水法获得的蒲公英提取物作为活性成分。 制备免疫刺激剂的方法包括以下步骤：通过热蒸馏水在90〜100℃的温度下提取蒲公英提取物。 蒲公英提取物可以单独使用或与通常使用的添加剂一起使用。
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Patent Agency Ranking
公开(公告)号：KR1020080081499A
公开(公告)日：2008-09-10
申请号：KR1020070021570
申请日：2007-03-05
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/064 , C12N1/18
Abstract: An exo-polysaccharide derived from a Saccharomyces spp. strain is provided to enhance various activities of neutrophil, macrophage and lymphocyte, thereby being widely applied to an animal immunity-enhancing agent, a vaccine supplemental additive and an adjuvant therapeutic agent. An animal immunity enhancing agent comprises exo-polysaccharides extracted from a culture material of a Saccharomyces spp. strain. A method for preparing the exo-polysaccharides derived from the Saccharomyces spp. strain comprises the steps of: (a) after suspending pure colonies obtained by culturing the Saccharomyces spp. strain in sterile saline solution, inoculating 1 ml of the suspension into 1,000 ml of yeast malt broth and culturing it at a temperature of 28 deg.C for 72 hours; (b) after adding two times amount of distilled water into a culture material obtained from the step(a) and mixing it, boiling the mixture at a temperature of 100 deg.C for 1 hour and centrifuging the boiled mixture under 5,000xg for 30 minutes to collect supernatant therefrom; and (c) after adding two times amount of ethyl alcohol to the supernatant and mixing it, leaving a mixture in a cool chamber at a temperature of 4 deg.C for 2 days and collecting precipitated polysaccharides therefrom.
Abstract translation: 衍生自酵母属（Saccharomyces spp。）的外多糖。 提供菌株以增强嗜中性粒细胞，巨噬细胞和淋巴细胞的各种活性，从而广泛应用于动物免疫增强剂，疫苗补充添加剂和辅助治疗剂。 动物免疫增强剂包括从酵母属的培养物质中提取的外多糖。 应变。 一种制备衍生自酵母属（Saccharomyces spp）的外多糖的方法。 菌株包括以下步骤：（a）悬浮通过培养酵母属（Saccharomyces spp）获得的纯菌落后。 在无菌生理盐水溶液中，将1ml悬浮液接种到1,000ml酵母麦芽培养液中，并在28℃的温度下培养72小时; （b）在向步骤（a）获得的培养物中加入两倍量的蒸馏水并混合后，在100℃的温度下将混合物煮沸1小时，并将煮沸的混合物在5,000xg下离心30分钟 分钟收集上清液; 和（c）在向上清液中加入两倍量的乙醇并混合后，在4℃的温度下在冷室中留下混合物2天，并从中收集沉淀的多糖。
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公开(公告)号：KR1020080068361A
公开(公告)日：2008-07-23
申请号：KR1020070006055
申请日：2007-01-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A plate agglutination tester and a method for measuring an immuno-reaction using the same are provided to decrease error of the result analysis in accordance with proficiency of a person performing diagnosis, show the degree of various immuno-reactions in an objective value and perform various statistical analyses, thereby being very useful for measuring the immuno-reaction. A plate agglutination tester consists of a transparent film and is characterized in that a section, which consists of a plurality of circles and where an antigen-antibody agglutination reaction occurs, is arranged and formed in horizontal and vertical directions on an upper side of the film. A method for measuring an immuno-reaction comprises the steps of: (a) mixing an antigen and an antibody specifically bound to the antigen with the plate agglutination tester to induce an agglutination reaction; (b) scanning the agglutination reaction induced plate agglutination tester and converting a scanning result into an image file; and (c) analyzing the obtained image file using a computer program capable of detecting fine agglutination reactants to numberize the intensity of the agglutination reaction. Further, the transparent film is an over head project film.
Abstract translation: 提供平板凝集试验机和使用其的免疫反应测定方法，根据进行诊断的人员的熟练程度来减少结果分析的误差，以目标值显示各种免疫反应的程度，并进行各种 统计分析，因此对测量免疫反应非常有用。 板凝集试验仪由透明膜构成，其特征在于，在膜的上侧，在水平和垂直方向上布置并形成由多个圆组成并且发生抗原 - 抗体凝集反应的部分 。 测定免疫反应的方法包括以下步骤：（a）将抗原与抗原特异性结合的抗体与平板凝集试验仪混合以诱导凝集反应; （b）扫描凝集反应诱导的板凝集测试仪并将扫描结果转换为图像文件; 和（c）使用能够检测细微凝集反应物的计算机程序来分析所获得的图像文件，以对凝集反应的强度进行编号。 此外，透明膜是顶部投影膜。
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148.发明公开
公开(公告)号：KR1020080062815A
公开(公告)日：2008-07-03
申请号：KR1020060138940
申请日：2006-12-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A target-organ array set and a method for diagnosing exposure to a brominated compound and intoxication by the brominated compound are provided to identify the intoxication and contamination degree of the brominated compound easily by identifying a change of a protein which shows specific change in an organ sensitively influenced by the brominated compound. A target-organ array set for diagnosing exposure to a brominated compound and intoxication by the brominated compound comprises: a control sample adhesion portion where at least one selected from the group consisting of pituitary tissue, a thyroid tissue, a liver tissue and a prostatic tissue of a normal group is attached to the surface; and an adhesion portion to be measured where a sample to be measured regarding a control group is adhered. An antibody set for diagnosing exposure to the brominated compound and intoxication by the brominated compound comprises at least one antibody selected from the group consisting of an anti-pregnane X receptor antibody, anti-apolipoprotein A-1 antibody, anti Bcl-2 antibody, anti-cytochrome P4501A1 antibody, anti UDP-glucuronosyltransferase 1A antibody, and anti androgen receptor antibody.
Abstract translation: 提供靶器官阵列组和用于诊断溴化化合物暴露和溴化化合物中毒的方法，以通过鉴定显示器官特异性变化的蛋白质的变化容易地识别溴化化合物的中毒和污染程度 受溴化合物的敏感影响。 用于诊断暴露于溴化化合物并被溴化化合物中毒的靶器官阵列组包括：对照样品粘附部分，其中选自垂体组织，甲状腺组织，肝组织和前列腺组织中的至少一种 正常组附着在表面上; 以及附着在待测量样品上的粘附部分。 用于诊断暴露于溴化化合物并被溴化化合物中毒的抗体集合包含至少一种选自抗孕烷X受体抗体，抗载脂蛋白A-1抗体，抗Bcl-2抗体，抗 - - 细胞色素P4501A1抗体，抗UDP-葡糖醛酸基转移酶1A抗体和抗雄激素受体抗体。
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公开(公告)号：KR100834026B1
公开(公告)日：2008-06-02
申请号：KR1020070001888
申请日：2007-01-08
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K39/0208 , C07K14/195
Abstract: A riemerella vaccine for preventing riemerella infection is provided to defend the infection of field pathogenic Riemerella anatipestifer which occurs frequently in a country and prevent ducks from being perished by the riemerella infection, thereby minimizing economical loss of a duck industry. A riemerella vaccine for preventing riemerella infection comprises an inactivated Riemerella anatipestifer and an immuno-enhancing agent, wherein the inactivated Riemerella anatipestifer is a strain selected from the group consisting of serovar type I, serovar type IV, serovar type VII, serovar type XVI and a mixture strain thereof. A method for preparing the riemerella vaccine comprises the steps of: (a) culturing Riemerella anatipestifer isolated from a suck; (b) adding an inactivating agent such as 0.3% formalized physiological saline solution(FPSS) to a culture solution obtained from the step(a) to inactivate the Riemerella anatipestifer; and (c) adding an immuno-enhancing agent to the inactivated Riemerella anatipestifer culture solution to prepare a mixture vaccine. Further, the immuno-enhancing agent is ISA70.
Abstract translation: 提供了一种用于预防痢疾杆菌感染的雷米他菌疫苗，用于捍卫一个国家频繁发生的野外致病性雷米他氏菌感染，并防止鸭子被莱梅里埃菌感染消灭，从而最大限度地减少了鸭业的经济损失。 用于预防雷米他里亚感染的莱米诺菌素疫苗包括灭活的雷米他氏菌和免疫增强剂，其中所述灭活的雷米他氏菌是选自I型血清型，IV型血清型，VII型血清型，血清型XVI和 其混合应变。 一种制备莱米酵母疫苗的方法包括以下步骤：（a）培养从吸吮物分离的烟草雷蒙酵母; （b）将灭活剂如0.3％正规化生理盐水溶液（FPSS）加入到从步骤（a）获得的培养溶液中以灭活烟草雷蒙酵母; 和（c）向灭活的雷神雷公藤培养液中加入免疫增强剂以制备混合疫苗。 此外，免疫增强剂是ISA70。
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公开(公告)号：KR100787830B1
公开(公告)日：2008-01-02
申请号：KR1020060007213
申请日：2006-01-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명자는 돈단독불활화백신의 면역효능을 향상시키기 위하여 불활화한 돈단독균체에 돈단독균 특이방어항원인 분비단백질을 정제, 농축하여 첨가한 돈단독불활화백신의 면역능에 미치는 효과를 시험하여 다음과 같은 결과를 얻었다.
돈단독균 배양상층액중의 정제단백항원의 항체형성능이 균체항원보다 월등하였으며 마우스 방어효과도 보다 우수하였다. 또한 돈단독불활화 항원에 정제단백항원을 첨가하므로서 항체가 및 방어가 모두가 향상되었다.
상기의 불활화항원과 정제단백 혼합항원에 돈단독 특이항체 또는 glucan 등 면역증강물질을 첨가하므로서 항체가 및 방어효과의 현저한 상승효과를 나타내었다.
마우스에서 면역원성 향상효과가 확인된 항원으로 제조한 돈단독불활화백신을 목적동물인 이유자돈에 접종하고 항체형성능 및 안전성을 시험한 바 불활화항원만으로 만든 백신보다 균질화항원에 항체를 첨가하거나 불활화 항원에 정제단백 및 항체를 첨가한 군의 항체가가 월등히 높았으며 시험기간 동안 발열 등 이상반응이 관찰되지 않아 안전성이 확인되었다.
돈단독 불활화백신, 정제단백항원, 돈단독 특이항체, 면역증강물질
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