公开(公告)号：KR1020100110046A

公开(公告)日：2010-10-12

申请号：KR1020090028413

申请日：2009-04-02

Applicant: 한국식품연구원

Inventor： 손동화 ,  김현구 ,  김선아 ,  하태열 ,  안지윤 ,  박민희 ,  주민정 ,  김미혜

IPC: C12P19/00 ,  A61K36/744

CPC classification number: C12P19/20 ,  A61K36/744 ,  C12Y302/01021

Abstract: PURPOSE: A method for preparing gardenia fruit extract with enhanced anti-inflammatory activity is provided to change a material by an enzyme reaction using beta-glucosidase. CONSTITUTION: A gardenia fruit extract with enhanced anti-inflammatory activity is prepared by reacting beta-glucosidase to gardenia fruit extract at 20 unit/ml, pH 4.6, and 45°C for 24 hours. The gardenia fruit extract is obtained by adding a solvent to gardenia fruit and treating with microwaves at 90W for 5 minutes. The solvent is a mixture solvent of water-ethanol. A pharmaceutical composition for anti-inflammation contains gardenia fruit extract as an active ingredient. A health food for preventing and treating inflammation contains gardenia fruit extract as an active ingredient.

Abstract translation: 目的：提供一种具有增强抗炎活性的栀子果实提取物的制备方法，以通过使用β-葡糖苷酶的酶反应来改变材料。 构成：通过β-葡萄糖苷酶将栀子果提取物以20单位/ ml，pH 4.6和45℃反应24小时制备具有增强抗炎活性的栀子果实提取物。 栀子果提取物通过向栀子果中加入溶剂并在90W下用微波处理5分钟获得。 溶剂是水 - 乙醇的混合溶剂。 用于抗炎的药物组合物含有栀子果提取物作为活性成分。 用于预防和治疗炎症的保健食品含有栀子果提取物作为活性成分。

公开(公告)号：KR1020090073669A

公开(公告)日：2009-07-03

申请号：KR1020070141680

申请日：2007-12-31

Applicant: 한국식품연구원

Inventor： 김남수 ,  조용진 ,  손동화

IPC: G01N35/00 ,  G01N33/53 ,  G01N33/68 ,  G01N33/48

Abstract: A crystal oscillator immunosensor measurement apparatus for the measurement of a C-reactive protein, and its method are provided to allow C-reactive protein to be measured in real time within a short time by sing a biomarker. A crystal oscillator immunosensor measurement apparatus for the measurement of a C-reactive protein comprises a reaction buffer solution tub; a supply unit which is connected to the reaction buffer solution tub, and supplies the reaction buffer solution of the reaction buffer solution tub to the C-reactive protein fixed to the crystal oscillator sensor chip of a reactive cell; a sample inlet hole which is connected to the supply unit; a reaction cell which comprises a crystal oscillator, an O-ring, and a holder; an oscillation module which oscillates the crystal oscillator inside the reaction cell and is connected to the crystal oscillator to transmit the vibration of the crystal oscillator to a vibration sensor; a vibration sensor which measures the vibration of the crystal oscillator of the reaction by the oscillation module; a computer which is connected to the vibration sensor to show the frequency variation of the C-reactive protein fixed to the sensor chip electrode of the crystal oscillator; and a waste liquid tank.

Abstract translation: 提供用于测量C-反应蛋白的晶体振荡器免疫传感器测量装置及其方法，以通过生物标记物通过生物标记物在短时间内实时测量C反应蛋白。 用于测量C反应蛋白的晶体振荡器免疫传感器测量装置包括反应缓冲溶液槽; 供给单元，其连接到反应缓冲溶液槽，并将反应缓冲溶液槽的反应缓冲溶液供应到固定在反应池的晶体振荡器传感器芯片上的C-反应蛋白; 连接到供应单元的样品入口孔; 包括晶体振荡器，O形环和保持器的反应单元; 振荡模块，其使晶体振荡器在反应单元内部振荡并连接到晶体振荡器，以将振荡器的振动传递到振动传感器; 振动传感器，其测量所述振荡模块对所述晶体振荡器的振动的反应; 连接到所述振动传感器以显示固定到所述晶体振荡器的所述传感器芯片电极的C反应蛋白的频率变化的计算机; 和废液罐。

公开(公告)号：KR1020030018168A

公开(公告)日：2003-03-06

申请号：KR1020010051749

申请日：2001-08-27

Applicant: 한국식품연구원

Inventor： 유익종 ,  손동화 ,  성기승 ,  전기홍 ,  조혜연 ,  양용진 ,  문성철 ,  장주현

IPC: A61K39/395

Abstract: PURPOSE: A method for producing eggs containing an anti-insulin antibody is provided, thereby producing eggs containing specific immunoglobulin yolk(IgY) for a long time. CONSTITUTION: The method for producing eggs containing an anti-insulin antibody comprises the steps of: pre-treating human insulin and conjugating the pre-treated human insulin to a carrier to prepare the insulin-protein conjugate; periodically injecting the conjugate together with an immune adjuvant and an emulsifying agent to laying hens, wherein the pre-treatment of human insulin is carried out by dialysis of human insulin with a 3,500Da membrane at 4deg. C for 24 hours; the carrier is ovalbumin or bovine serum albumin; EDAC(1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) is coupled with the insulin-protein conjugate; and FCA(Freund's complete adjuvant) is used as an emulsifying agent in initial immunization and FIA(Freund's incomplete adjuvant) is used as another emulsifying agent in later immunization.

Abstract translation: 目的：提供含有抗胰岛素抗体的卵的生产方法，由此产生长时间含有特异性免疫球蛋白（IgY）的卵。 构成：含有抗胰岛素抗体的蛋的生产方法包括以下步骤：预处理人胰岛素并将预处理的人胰岛素与载体缀合以制备胰岛素 - 蛋白质缀合物; 将结合物与免疫佐剂和乳化剂定期注射到产蛋鸡，其中人胰岛素的预处理是通过用4,500g的3,500Da膜透析人胰岛素进行的。 C 24小时; 载体为卵白蛋白或牛血清白蛋白; EDAC（1-乙基-3-（3-二甲基氨基丙基）碳二亚胺）与胰岛素 - 蛋白质结合物偶联; 和FCA（弗氏完全佐剂）在初次免疫中用作乳化剂，并且在后续免疫中使用FIA（弗氏不完全佐剂）作为另外的乳化剂。

公开(公告)号：KR1020030000261A

公开(公告)日：2003-01-06

申请号：KR1020010035945

申请日：2001-06-22

Applicant: 한국식품연구원

Inventor： 김영붕 ,  노정해 ,  성기승 ,  한찬규 ,  손동화 ,  이남형 ,  백반석 ,  신태범 ,  고성찬 ,  허강준

IPC: A61K39/40

Abstract: PURPOSE: A Method for the production of an egg containing anti-Edwardsiella tarda IgY, anti-Streptococcus iniae IgY and Mycobacterium bovis IgY simultaneously, an egg produced thereby and a fish feed containing specific IgY thereof are provided. The produced egg and fish feed have excellent prevention effect on a flatfish disease. CONSTITUTION: An emulsion containing Edwardsiella tarda IgY, anti-Streptococcus iniae IgY, Mycobacterium bovis IgY and aluminium oxide in ratio of 3.0:3.0:1.0:3.0 is inoculated into a chicken in the amount of 1.0ml one time, and then, from a second time, the above emulsion and an adjuvant(ISA25) are inoculated together thereinto in the amount of 1.0ml at intervals of 2 weeks to produce an egg containing specific IgY. Egg yolk is then put up in a vessel, stirred in the equal amount of alkali ion water(pH 10.0), left alone for a specified period of time and then the supernatant is ultra-filtrated and freeze-dried after removing a fat layer floated on the upper layer to produce soluble IgY powder.

Abstract translation: 目的：提供一种产生含有抗Edwardsiella tarda IgY，抗链球菌IgY和牛分枝杆菌IgY的蛋的方法，由此产生的蛋和含有其特异性IgY的鱼饲料。 生产的鸡蛋和鱼饲料对鲽鱼病具有极好的预防作用。 构成：将含有Edwardsiella tarda IgY，抗链球菌IgY，牛分枝杆菌IgY和氧化铝的比例为3.0:3.0:1.0:3.0的乳剂一次接种到鸡1.0ml中，然后从 第二次，将上述乳剂和佐剂（ISA25）以2周的间隔以1.0ml的量接种在一起，以产生含有特异性IgY的蛋。 然后将蛋黄放在容器中，在等量的碱性离子水（pH10.0）中搅拌一段时间，然后将上清液超滤并冷冻干燥，除去脂肪层漂浮 在上层产生可溶性IgY粉末。

公开(公告)号：KR100338922B1

公开(公告)日：2002-05-30

申请号：KR1020000011226

申请日：2000-03-07

Applicant: 주식회사 씨트리 ,  한국식품연구원

Inventor： 이남형 ,  유익종 ,  김영붕 ,  한찬규 ,  노정해 ,  성기승 ,  손동화

IPC: C12P21/00

Abstract: 본 발명은 위염 및 십이지장염을 일으키는 5종 균을 항원화시켜, 산란계에 각각 접종하여 위염 및 십이지장염을 예방할 수 있는 복합 특수 면역 단백질을 생산하는 방법에 관한 것으로, 본 발명에 의해 제조된 5종의 복합 특수 면역 단백질은 위염과 십이지장염 예방에 탁월한 효능이 있으며, 항생제를 사용하여 일단 치료되더라도 재발율이 매우 높은 위염과 십이지장염을 계란에서 생산된 면역 단백질에 의해 예방 효과가 있다는데 큰 의의가 있다.