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    가금티푸스균 감별 동정용 유전자 진단법
    12.
    发明公开
    가금티푸스균 감별 동정용 유전자 진단법 有权
    沙门氏菌肠杆菌葡萄球菌葡萄球菌，PULLORUM和生物葡萄球菌活菌株的差异鉴定

    公开(公告)号：KR1020110044427A

    公开(公告)日：2011-04-29

    申请号：KR1020090101085

    申请日：2009-10-23

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 강민수 ,  권용국 ,  정병열 ,  김애란 ,  임춘태 ,  이강무 ,  안병기 ,  권준헌

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: Y02A50/451 ,  C12Q1/6888 ,  C12N15/11 ,  C12Q1/6844

    Abstract: PURPOSE: A method for detecting Salmonella gallinarum or probiotics for Salmonella gallinarum vaccine is provided to quickly detect Salmonella gallinarum. CONSTITUTION: A method for detecting Salmonella gallinarum or probitocs Salmonella gallinarum comprises: a step of preparing DNA from a biological sample; a step of performing PCR using the DNA as a temperate and primer; and a step of analyzing DNA fragment through from PCR product.

    Abstract translation: 目的：提供一种检测沙门氏菌沙门氏菌或益生菌沙门氏菌疫苗的方法，以快速检测沙门氏菌。 构成：检测沙门氏菌或前列腺素沙门氏菌的方法包括：从生物样品制备DNA的步骤; 使用DNA作为温带和引物进行PCR的步骤; 以及通过PCR产物分析DNA片段的步骤。

    동물의 면역능을 증강시키는 감초 추출물 및 그 제조 방법
    14.
    发明公开
    동물의 면역능을 증강시키는 감초 추출물 및 그 제조 방법 无效
    葡萄糖提取物提高动物免疫力及其制备方法

    公开(公告)号：KR1020080106789A

    公开(公告)日：2008-12-09

    申请号：KR1020070054645

    申请日：2007-06-04

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 이희수 ,  김종만 ,  임숙경 ,  김재명 ,  박신영 ,  임춘태 ,  장금찬 ,  정석찬

    IPC: A61K36/236 ,  A61P37/02

    Abstract: A Glycyrrhiza hot water extract, a method for preparing the extract, and an immune enhance containing the extract are provided to improve the immune increasing activity of animals. A Glycyrrhiza hot water extract is prepared by adding 1,000 mL of distilled water per 100 g of the dried Glycyrrhiza to soak Glycyrrhiza for 20-30 min and boiling it down for 150 min to obtain an extract; adding 800 mL of distilled water per 100 g of the dried Glycyrrhiza to the Glycyrrhiza remaining after the extraction and boiling it down for 150 min to obtain an extract; adding 700 mL of distilled water per 100 g of the dried Glycyrrhiza to the Glycyrrhiza remaining after the extraction and boiling it down for 150 min to obtain an extract; collecting the obtained extract and concentrating it; and dry-freezing the concentrated one.

    Abstract translation: 提供甘草热水提取物，制备提取物的方法和含有提取物的免疫增强物，以提高动物的免疫增加活性。 通过向100g甘草中加入1000mL蒸馏水，将Glycyrrhiza浸泡20-30分钟，煮沸150分钟，得到甘草热水提取物，得到提取物; 向100g甘草中加入800毫升蒸馏水，然后将其蒸馏除去150分钟，得到提取物; 向100g甘草中加入700毫升蒸馏水至提取后剩余的甘草，煮沸150分钟，得到提取物; 收集所得提取物并浓缩; 并干燥浓缩浓缩的。

    장출혈성대장균(ＥＨＥＣ) 감염증 예방용 톡소이드 백신
    15.
    发明公开
    장출혈성대장균(ＥＨＥＣ) 감염증 예방용 톡소이드 백신 有权
    防止感冒性肠胃炎的感染性疾病

    公开(公告)号：KR1020070104720A

    公开(公告)日：2007-10-29

    申请号：KR1020060036588

    申请日：2006-04-24

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 이희수 ,  임숙경 ,  조윤상 ,  임춘태 ,  정석찬

    IPC: C12N1/20

    Abstract: An Enterohemorrhagic Escherichia coli(EHEC) strain isolated from Korean native cattle is provided to be used as a vaccine strain. And a toxoid vaccine for preventing an infectious disease of EHEC comprising an inactivated Shiga toxin produced from the strain is provided to prevent hemorrhagic diarrhea of a calf and induce the decrease of the infection in cattle, thereby being ultimately used for preventing an infectious disease of EHEC. An EC377 strain selected from EHEC derived from Korean native cattle produces shigatoxins 1(stx1) and has a plasma type of 055. An EC611 strain selected from the EHEC derived from Korean native cattle produces stx2 and has a plasma type of 06. An EC381 strain selected from the EHEC derived from Korean native cattle produces stx1 and stx2 and has a plasma type of 0153. 06. 55. A method for promoting the generation of Shiga toxins is characterized in that 0.5-1.0mug/ml of Mitomycin C is added during the culturing of the EHEC strains. A toxoid vaccine for preventing an infectious disease of EHEC comprises at least one Shiga toxins cStx1 and cStx2 toxoid antigen prepared by treating the shigatoxins with 0.2-0.5% of glutaraldehyde for 1-3 hours and then detoxicating it with 0.2-1.0% of lysine.

    Abstract translation: 提供从韩国本地牛分离的肠出血性大肠杆菌（EHEC）菌株用作疫苗株。 并提供一种用于预防由该菌株产生的包含灭活的志贺毒素的EHEC感染性疾病的类毒素疫苗，以防止小牛的出血性腹泻并诱导牛的感染减少，从而最终用于预防EHEC的感染性疾病 。 选自源自韩国本地牛的EHEC的EC377菌株产生了起始的黄曲霉毒素1（stx1），血浆类型为055.选自来自韩国本地牛的EHEC的EC611菌株产生stx2，血浆类型为0.06。EC381菌株 选自源自韩国本地牛的EHEC产生stx1和stx2，并具有等离子体类型0153. 06. 55.一种用于促进滋贺毒素生成的方法，其特征在于在0.5-10.0μg/ ml丝裂霉素C中加入 培养EHEC菌株。 用于预防EHEC传染病的类毒素疫苗包含至少一种通过用0.2-0.5％戊二醛处理志贺菌素1-3小时制备的志贺毒素cStx1和cStx2类毒素抗原，然后用0.2-1.0％的赖氨酸对其进行解毒。

    호중구를 이용한 병원균의 약독화 방법
    19.
    发明公开
    호중구를 이용한 병원균의 약독화 방법 有权
    使用中性粒细胞衰竭的方法

    公开(公告)号：KR1020100110434A

    公开(公告)日：2010-10-13

    申请号：KR1020090028760

    申请日：2009-04-03

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 이희수 ,  김애란 ,  임숙경 ,  조윤상 ,  임춘태 ,  윤민 ,  정석찬

    IPC: A61K39/112 ,  C12N5/07 ,  A61K39/10 ,  A61P37/00

    CPC classification number: A61K39/0275 ,  A61K2039/522

    Abstract: PURPOSE: A method for attenuating pathogen by co-culture of pathogen and neutrophil is provided to attenuate without high technology and safely apply to human body. CONSTITUTION: A pathogen is attenuated by co-culture of pathogen and neutrophils. The neutrophil is isolated from poultry, pig, lamb, horse, cat, dog or human. A method for co-culture of pathogen and neutrophil comprises: a step of co-culture of neutrophili and pathogen for 10 minutes to two hours; a step of killing pathogen; and a step of collecting and dissolving neutrophil. The attenuated pathogen is ST31-N5th-1(KACC 91447P) or ST31-N20th-B10th-12(KACC 91448P). A vaccine composition contains attenuated pathogenic mutant.

    Abstract translation: 目的：通过病原体和嗜中性粒细胞的共培养来减轻病原体的方法，无需高技术的减毒，安全应用于人体。 构成：通过病原体和嗜中性粒细胞的共培养来减轻病原体。 嗜中性粒细胞与家禽，猪，羊，马，猫，狗或人类分离。 病原体和嗜中性粒细胞的共培养方法包括：将嗜中性粒细胞和病原体共培养10分钟至2小时的步骤; 杀死病原体的一个步骤; 和收集和溶解嗜中性粒细胞的步骤。 减毒病原体是ST31-N5th-1（KACC91447P）或ST31-N20th-B10th-12（KACC91448P）。 疫苗组合物含有减毒致病突变体。

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