Abstract:
PURPOSE: A probe set for diagnosing or detecting LRSV(lychnis ring-spot virus) is provided to minimize damage due to viral diseases in crops and to increase product yield. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting LRSV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1307-1311 and complementary oligonucleotides thereof. A method for diagnosing or detecting LRSV comprises: a step of isolating RNA from a sample and synthesizing cDNA; a step of hybridizing cDNA with the probe set; and a step of detecting hybridization.
Abstract:
PURPOSE: A probe set for diagnosing or detecting TBSV(Tomato bushy stunt virus) is provided to minimize damage to viral diseases in crops and to enhance the yield of crops. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting TBSV contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence number 3432-3438 and complementary oligonucleotides thereof. A method for diagnosing or detecting TBSV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA; a step of hybridizing the cDNA and the probe set; and a step of detecting hybridization. [Reference numerals] (AA) Koko tomato; (BB) Host: Tenten tomato
Abstract:
PURPOSE: A plant tissue-specific BrLRP1 promoter is provided to effectively produce a gene product during a growth stage under a certain condition. CONSTITUTION: A transgenic Brassica plant in which an expression vector is introduced contains a BrLRP1 promoter with a nucleic acid sequence in sequence number 1. The expression vector is a pPBrLRP1 vector.
Abstract:
PURPOSE: A BrREF promoter which is specific to cotyledon and hypocotyls is provided to specifically induce the expression of a gene in a certain tissue, and to effectively produce a gene product under a predetermined condition. CONSTITUTION: A promoter contains a nucleic acid sequence in sequence number 1. An expression vector contains the promoter, and is pPBrREF. A cell is transformed with the expression vector. The cell is an Agrobacterium sp. microorganism or a plant cell. A transgenic plant contains the expression vector. The plant is dicotyledones.
Abstract:
PURPOSE: A specific primer for soybean virus multiplex diagnosis is provided to reduce diagnosing cost and efforts by propagation of diagnosing method for various viruses caused in the soybean. CONSTITUTION: A specific primer for soybean virus multiplex diagnosis comprises A pair of primers represented by the sequence number 1(SEQ ID NO:1) and the sequence number 2, a pair of primers represented by the sequence number 3, 4, 5, and 6, a pair of primers represented by the sequence number 7 and 8, and a pair of primers represented by the sequence number 9 and 10. The specific primer for soybean virus multiplex diagnosis additionally includes a pair of primers represented by 11 and 12. The specific primer can concurrently diagnose AMV, SYMMV and USV3.
Abstract translation:目的:提供大豆病毒多重诊断的具体引物,以减少大豆中引起的各种病毒诊断方法的诊断成本和努力。 构成:用于大豆病毒多重诊断的具体引物包括由序列号1(SEQ ID NO:1)和序列号2表示的一对引物,由序列号3,4,5和 6,由序列号7和8表示的一对引物和由序列号9和10表示的一对引物。大豆病毒多重诊断的特异性引物另外包括由11和12表示的一对引物。 特异性引物可同时诊断AMV,SYMMV和USV3。
Abstract:
A method for manufacturing intron based polymorphism primer set through in-silico analysis is provided to quickly and massively produce molecular marker and develop novel species. A method for manufacturing an intro based polymorphism primer set comprises: a step of comparing gene of Brassicaceae plant with expressed gene sequence tag to distinguish exon and intron in the plant gene; a step of determining common axon; and a step of performing in-silico simulation using intron between the axons to produce the intron based polymorphism primer. The Brassicaceae plant is Chinese cabbage, Brassica oleracea var. or broccoli.
Abstract:
본 발명은 배추과 작물의 품종 구분 및 유용표지인자 개발 수단으로서의 왜성전이인자인 서열번호1 기재의 TRIM-Br1 또는 서열번호2 기재의 TRIM-Br2, 이를 이용한 배추과 작물의 품종 구분에 사용되는 DNA 표지인자 제작용 프라이머인 서열번호3~10 중 어느 하나의 서열을 갖는 Br1n2DP-R 또는 서열번호11~14 중 어느 하나의 서열을 갖는 Br1n2DP-L, 및 이를 이용한 배추과 작물의 품종 구분 방법에 관한 것으로서, 보다 상세하게는 배추과 작물의 유전육종 과정에서 유용한 표지인자를 탐색하거나 유전자 지도 작성 및 품종구분에 효율적으로 활용할 수 있는 것으로서 배추과 작물의 유전자 밀집지역 전반에 골고루 삽입되어 있는 TRIM-Br1 및 TRIM-Br2 전이인자, 상기 전이인자 TRIM-Br1 및 TRIM-Br2를 이용한 DNA 표지인자를 제작하는데 사용되는 프라이머, 및 TRIM-Br1 및 TRIM-Br2의 특이 염기서열을 이용한 배추과 작물의 품종 구분방법에 관한 것이다. 배추, 왜성 전이인자, TRIM-Br1, TRIM-Br2, TRIM 디스플레이