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    아퀴펙스 파이로필러스 슈퍼옥사이드 디스뮤타제 유전자 서열 및 그로부터 발현되는 단백질
    11.
    发明公开
    아퀴펙스 파이로필러스 슈퍼옥사이드 디스뮤타제 유전자 서열 및 그로부터 발현되는 단백질 失效
    Acquepex pyrophyllulose超氧化物歧化酶基因序列和由此表达的蛋白质

    公开(公告)号：KR1019980065913A

    公开(公告)日：1998-10-15

    申请号：KR1019970001140

    申请日：1997-01-16

    Applicant: 한국과학기술연구원

    Inventor： 한예선 ,  유연규 ,  김성호 ,  임재환 ,  류재련 ,  최인걸

    IPC: C12N15/53

    Abstract: 초고온 미생물인 아퀴펙스 파이로필러스(Aquifex pyrophilus)의 슈퍼옥사이드 디스뮤타제 유전자 서열 및 그 서열로부터 발현되는 단백질이 제공된다.
    슈퍼옥사이드 디스뮤타제는 산소에 의한 세포의 손상을 막아주는 중요한 방어 기작에 관여하므로, 염증, 자기 면역 질환 및 염색체 손상 등의 치료에 의약으로 사용될 수 있다. 특히, 에이. 파이로필러스는 최적 온도 85℃에서 성장하는 초내열성 세균의 일종이므로, 그의 슈퍼옥사이드 디스뮤타제의 열안정성도 다른 미생물에 비해 아주 높아 제약 분야에서 폭 넓게 응용될수 있다.

    아시네토박토 종 ＳＹ－０１의 리파제 코딩 유전자 및이로부터 발현되는 리파제
    12.
    发明授权
    아시네토박토 종 ＳＹ－０１의 리파제 코딩 유전자 및이로부터 발현되는 리파제 失效
    雅네토종종종SY-01파딩딩및및및종종종종종종종종종종종종

    公开(公告)号：KR100468547B1

    公开(公告)日：2005-01-29

    申请号：KR1020020045165

    申请日：2002-07-31

    Applicant: 한국과학기술연구원

    Inventor： 한예선 ,  한수진 ,  백정호 ,  신평균 ,  정찬성 ,  성문희 ,  조현성 ,  이병석

    IPC: C12N15/55

    Abstract: PURPOSE: Provided are a gene coding lipase of Acinetobacter species SY-01 with stereoselective substrate specificity and a protein expressed therefrom. CONSTITUTION: A gene is characterized by coding amino acid sequence of lipase of Acinetobacter species SY-01, represented by the SEQ ID NO:2. A transformed Bacillus subtilis 168/pSYLipSM(1) (KCTC 10267BP) is obtained by transformation with a recombinant vector plasmid pSYLipSM(1) containing the gene coding the lipase of Acinetobacter species SY-01. The lipase of Acinetobacter species SY-01 is manufactured by the steps of culturing the transformed bacillus subtilis, and isolating and purifying expressed lipase of Acinetobacter species SY-01 from the cultured cell.

    Abstract translation: 目的：提供具有立体选择性底物特异性的不动杆菌种SY-01的编码脂肪酶的基因和由其表达的蛋白质。 组成：基因的特征在于编码不动杆菌属物种SY-01的脂肪酶的氨基酸序列，由SEQ ID NO：2表示。 通过用含有编码不动杆菌属物种SY-01的脂肪酶的基因的重组载体质粒pSYLipSM（1）转化获得转化的枯草芽孢杆菌168 / pSYLipSM（1）（KCTC 10267BP）。 不动杆菌菌种SY-01的脂肪酶是通过培养转化的枯草芽孢杆菌，从培养细胞中分离纯化不动杆菌菌种SY-01的表达脂肪酶。

    아시네토박토종 ＳＹ-０１ 리파제 변이체 코딩 유전자 및 이로부터 발현되는 리파제
    13.
    发明公开
    아시네토박토종 ＳＹ-０１ 리파제 변이체 코딩 유전자 및 이로부터 발현되는 리파제 失效
    基因编码ACIETOBACTER物种SY-01，BACILLUS SUBTILIS 168 / pSYLipM58（KCTC 10422BP）的变异体和含有其的突变体脂肪酶显示改善的酶活性

    公开(公告)号：KR1020040083588A

    公开(公告)日：2004-10-06

    申请号：KR1020030018093

    申请日：2003-03-24

    Applicant: 한국과학기술연구원

    Inventor： 한예선 ,  한수진 ,  정찬성 ,  임혜원 ,  조현성 ,  이병석 ,  양은경

    IPC: C12N15/55

    CPC classification number: C12N9/20 ,  C12N15/75 ,  C12Y301/01003

    Abstract: PURPOSE: Provided are a gene encoding mutant lipase of acinetobacter species SY-01, Bacillus subtilis 168/pSYLipM58 (KCTC 10422BP) as a transformant containing the same, and mutant lipase expressed therefrom displaying improved enzyme activity. The transformant is useful as a bio-catalyst. CONSTITUTION: The gene encoding mutant lipase of Acinetobacter species SY-01 has the nucleotide sequence set forth in SEQ ID NO:2. A recombinant vector of Acinetobacter species SY-01, plasmid pSYLipM58, comprises the gene encoding mutant lipase of Acinetobacter species SY-01. The transformant, Bacillus subtilis 168/pSYLipM58(KCTC 10422BP), contains the plasmid pSYLipM58. The method for producing the mutant lipase of Acinetobacter species SY-01 comprises the steps of: culturing Bacillus subtilis 168/pSYLipM58(KCTC 10422BP); and recovering and purifying the mutant lipase of Acinetobacter species SY-01 expressed from the cultured microorganism.

    Abstract translation: 目的：提供编码不动杆菌属SY-01，枯草芽孢杆菌168 / pSYLipM58（KCTC10422BP）的突变体脂肪酸的基因，其为含有该不动杆菌属物种的转化体，由表达改良的酶活性的突变体脂肪酶表达。 转化体可用作生物催化剂。 构成：编码不动杆菌属SY-01的突变体脂肪酶的基因具有SEQ ID NO：2所示的核苷酸序列。 不动杆菌属SY-01，质粒pSYLipM58的重组载体包含编码不动杆菌属SY-01突变体脂肪酶的基因。 转化体枯草芽孢杆菌168 / pSYLipM58（KCTC10422BP）含有质粒pSYLipM58。 生产不动杆菌属SY-01突变型脂肪酶的方法包括以下步骤：培养枯草芽孢杆菌168 / pSYLipM58（KCTC 10422BP）; 并回收和纯化由培养的微生物表达的不动杆菌属SY-01的突变脂肪酶。

    고 광학선택성을 가지는시스-2-(브로모메틸)-2-(2,4-디클로로페닐)-13-디옥소란-4-메틸알콜의 제조방법
    15.
    发明公开
    고 광학선택성을 가지는시스-2-(브로모메틸)-2-(2,4-디클로로페닐)-13-디옥소란-4-메틸알콜의 제조방법 有权
    制备具有高光学选择性的CIS-2-（BROMOMETHYL）-2-（2,4-二氯苯基） - 13-二氧杂环戊烯-4-甲醇的方法

    公开(公告)号：KR1020040006307A

    公开(公告)日：2004-01-24

    申请号：KR1020020040325

    申请日：2002-07-11

    Applicant: 한국과학기술연구원 ,  한올바이오파마주식회사 ,  경동제약 주식회사

    Inventor： 정찬성 ,  한예선 ,  윤문영 ,  조현성 ,  이병석

    IPC: C12P17/04

    Abstract: PURPOSE: A process for preparing cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 13-dioxolane- 4-methyl alcohol having high optical selectivity is provided. A stereo-selective enzyme maintains its maximum activity because the reaction product is rapidly removed from the reaction solution during the reaction in order not to inhibit the activity of the enzyme. CONSTITUTION: A process for preparing cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 13-dioxolane- 4-methyl alcohol having high optical selectivity comprises hydrolysis of cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 1,3-dioxolane- 4-methyl acetate using lipase produced from Acinetobacter sp. SY-01(KFCC-11111), and recovering an alcohol product as a reaction product from the reaction solution during the reaction, wherein the concentration of the alcohol product is 0.1 to 300 mg/l; the reaction product is recovered when the conversion rate of the hydrolysis reaction is 40 to 60%; the hydrolysis reaction is carried out under conditions of 40 to 60 deg. C, pH 7.0 to 9.0 and 100 to 300 rpm; and the reaction product is recovered by column chromatography.

    Abstract translation: 目的：提供具有高光学选择性的顺式-2-（溴甲基）-2-（2,4-二氯苯基）-1,3-二氧戊环-4-甲醇的方法。 立体选择性酶维持其最大活性，因为在反应期间快速从反应溶液中除去反应产物，以便不抑制酶的活性。 构成：具有高光学选择性的顺式-2-（溴甲基）-2-（2,4-二氯苯基）-1,3-二氧戊环-4-甲醇的制备方法包括顺式-2-（溴甲基）-2-（ 2,4-二氯苯基）-1,3-二氧戊环-4-甲基乙酸酯，使用由不动杆菌属（Acinetobacter sp。）生产的脂肪酶。 SY-01（KFCC-11111），并且在反应期间从反应溶液中回收作为反应产物的醇产物，其中醇产物的浓度为0.1至300mg / l; 当水解反应的转化率为40〜60％时回收反应产物; 水解反应在40〜60℃的条件下进行。 C，pH 7.0至9.0和100至300rpm; 并通过柱色谱法回收反应产物。

    클루이베로마이세스 마르시아누스로부터 분리 및 정제된 카르보닐 환원효소 및 그 제조 방법
    16.
    发明公开
    클루이베로마이세스 마르시아누스로부터 분리 및 정제된 카르보닐 환원효소 및 그 제조 방법 失效
    氧化还原血红蛋白血症和分离及其纯化方法

    公开(公告)号：KR1020030064159A

    公开(公告)日：2003-07-31

    申请号：KR1020020004657

    申请日：2002-01-26

    Applicant: 한국과학기술연구원

    Inventor： 고훈영 ,  한예선 ,  차주환 ,  김욱현 ,  이종수 ,  윤홍철

    IPC: C12N9/02

    CPC classification number: C12N9/0006

    Abstract: PURPOSE: A carbonyl reductase of Kluyveromyces marxianus and an isolation and purification method thereof are provided, thereby higher stereo-selectively, cheaply and rapidly carrying out the reduction process. CONSTITUTION: A carbonyl reductase isolated and purified from Kluyveromyces marxianus has the properties of the size of 40 to 42 kDa, the active temperature of 25 to 35 deg. C, and the active pH of 6.5 to 7.5, wherein the carbonyl reductase contains the amino acid sequence of Thr-Phe-Thr-Val-Val-Thr-Gly in the amino-terminal. A method for isolating and purifying the carbonyl reductase of Kluyveromyces marxianus comprises the steps of: (1) culturing Kluyveromyces marxianus; (2) centrifuging the cultured medium to collect the pellet and pulverizing the pellet; and (3) centrifuging the pulverized cell extract to collect the supernatant, and subjecting the supernatant to column chromatography to isolate an active fraction containing active material to substrate, wherein the column uses Q sepharose, phenyl sepharose, high-trap blue and gel filtration chromatography, sequentially.

    Abstract translation: 目的：提供马克斯克鲁维酵母的羰基还原酶及其分离和纯化方法，从而立体选择性更好，成本低廉且快速地进行还原过程。 构成：从马克斯克鲁维酵母分离和纯化的羰基还原酶具有40至42kDa大小的性质，活性温度为25至35度。 C，活性pH为6.5〜7.5，其中羰基还原酶含有氨基末端的Thr-Phe-Thr-Val-Val-Thr-Gly的氨基酸序列。 分离和纯化马克斯克鲁维酵母的羰基还原酶的方法包括以下步骤：（1）培养马克斯克鲁维酵母; （2）离心培养基以收集颗粒并粉碎颗粒; （3）将粉碎的细胞提取物离心以收集上清液，并将上清液进行柱色谱以将含有活性物质的活性级分与底物分离，其中该柱使用Q琼脂糖凝胶，苯基琼脂糖凝胶，高陷阱蓝和凝胶过滤色谱 ，顺序。

    호열성 리파제를 생산하는 균주
    17.
    发明公开
    호열성 리파제를 생산하는 균주 失效
    微生物菌株生产热成熟脂肪

    公开(公告)号：KR1020020017066A

    公开(公告)日：2002-03-07

    申请号：KR1020000050149

    申请日：2000-08-28

    Applicant: 한국과학기술연구원

    Inventor： 신평균 ,  윤문영 ,  박호일 ,  정찬성 ,  한예선

    IPC: C12N1/20

    Abstract: PURPOSE: Provided is a microorganism strain producing thermophile lipase. The above strain is obtained from the soil samples near the industrial complex, identified as a novel lipase producing strain different from known Burkholderia cepacia and named as SY-02(Burkholderia cepacia SY-02). It shows thermo-stability and high enzyme activity at the higher temperature than the melting point of fat and is thus widely useful as a lipase producing strain in the field of industry. CONSTITUTION: The strain Burkholderia cepacis SY-02(KFCC-111210) produces thermophilic lipase which is secreted into the exterior of a cell of Burkholderia cepacia SY-02, and shows activity at 40-80 deg.C, preferably 70 deg.C.

    Abstract translation: 目的：提供一种生产嗜热脂肪酶的微生物菌株。 上述菌株从工业化合物附近的土壤样品中获得，鉴定为与已知的洋葱伯克霍尔德菌（Burkholderia cepacia）不同的新型脂肪酶生产菌株，命名为SY-02（洋葱伯克霍尔德菌（SYK））。 它在比脂肪的熔点高的温度下显示出热稳定性和高酶活性，因此在工业领域中广泛用作脂肪酶生产菌株。 构成：洋葱伯克霍尔德菌SY-02（KFCC-111210）产生嗜热脂肪酶，其分泌到洋葱伯克霍尔德菌SY-02细胞的外部，并在40-80℃，优选70℃显示出活性。

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