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    11.
    发明专利
    未知

    公开(公告)号：ES2075861T3

    公开(公告)日：1995-10-16

    申请号：ES90116380

    申请日：1990-08-27

    Applicant: ABBOTT LAB

    Inventor： KHALIL OMAR S ,  ZUREK THOMAS F ,  GENGER KEVIN R ,  PEPE CURTIS J ,  JOU YI-HER ,  COTTER STEPHEN M

    IPC: G01N21/76 ,  G01N33/52 ,  G01N33/543 ,  G01N33/576 ,  G01N33/58

    Abstract: Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS
    14.
    发明专利
    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS 未知

    公开(公告)号：AU620554B2

    公开(公告)日：1992-02-20

    申请号：AU2006988

    申请日：1988-07-27

    Applicant: ABBOTT LAB

    Inventor： JOU YI-HER ,  HU ROGER C ,  LAGOCKI PETER A

    IPC: G01N33/544 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  G01N33/76 ,  C07K15/12 ,  G01N33/53 ,  G01N33/563 ,  G01N33/92

    Abstract: The present invention provides for novel homogeneous immunoassay systems involving complement-mediated lysis of marker-encapsulating lipid vesicles (liposomes) for detection of analyte in a fluid sample. Antibody sensitized liposomes (the first reagent) are sequentially incubated with an analyte-containing sample, and optionally "dummy" liposomes, which do not contain encapsulated marker, a second antibody (the second reagent), and finally with a complement source such as plasma. Complement is activated by the liposome-antibody-antigen-second antibody complex causing liposome lysis and a concomitant release of marker. Antibody of the first reagent may be an anti-analyte F(ab min )2 antibody fragment, or an anti-analyte Fab min antibody fragment. Antibody of the second reagent may be provided in either soluble form, or in insoluble form e.g., bound onto carboxylated polystyrene particles or coupled to a third antibody in the form of a "double antibody" immune precipitate. Also provided are methods for preparing antibody sensitized liposomes in the presence of a polysaccharide capable of forming a reversible gel and methods for preparing derivatized Fab min antibody fragments for coupling to lipid vesicles.

    ION-CAPTURE ASSAYS AND DEVICES
    15.
    发明专利
    ION-CAPTURE ASSAYS AND DEVICES 未知

    公开(公告)号：AU609241B2

    公开(公告)日：1991-04-26

    申请号：AU2866089

    申请日：1989-01-20

    Applicant: ABBOTT LAB

    Inventor： HILTIBRAN ROBERT G ,  JOU YI-HER ,  KLINE STEVEN J ,  SCHULTZ STEVEN GEORGE ,  STROUPE STEPHEN DENHAM

    IPC: G01N33/532 ,  G01N33/538 ,  G01N33/543 ,  G01N33/574 ,  G01N33/68 ,  C07K17/00 ,  G01N33/74 ,  G01N33/76

    Abstract: This invention presents novel separation and assay procedures which allows both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves an analyte-specific soluble capture reagent, that is conjugated to a charged substance, and an insoluble solid phase material that is oppositely charged. A fluid sample suspected of containing the analyte is mixed with the capture reagent in solution to form a charged capture reagent/analyte complex. When binding is complete, the solution is contacted to the oppositely charged solid phase material to attract, attach, and separate the capture reagent/analyte complex from the fluid sample. With the appropriate indicator reagent, i.e., a second analyte-specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed. The assay reaction complex can be separated from the solution by contact with the oppositely charged solid phase material, and the presence or amount of analyte is monitored by detecting the label of the indicator reagent.

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