公开(公告)号：EP2569453A4

公开(公告)日：2013-11-20

申请号：EP11781412

申请日：2011-05-16

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  ZIMMERMANN BERNHARD

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12P19/34 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2600/118 ,  C12Q2565/113 ,  C12Q2527/125 ,  C12Q2525/204 ,  C12Q2525/307 ,  C12Q2525/15 ,  C12Q2521/507

Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

Abstract translation: 本发明提供了选择性富集生物样品的方法，所述生物样品用于短核酸，例如母体样品中的胎儿DNA或来自癌症患者的生物样品中的凋亡DNA，并随后分析短核酸的基因型，突变和/ 或非整倍体。

公开(公告)号：EP2379753A4

公开(公告)日：2012-05-30

申请号：EP10732056

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

公开(公告)号：EP2569447A4

公开(公告)日：2013-11-27

申请号：EP11780930

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  PIEPRZYK MARTIN

Inventor： PIEPRZYK MARTIN ,  JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  G01N33/52 ,  G06F19/18

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

公开(公告)号：SG185544A1

公开(公告)日：2012-12-28

申请号：SG2012083432

申请日：2011-05-16

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  ZIMMERMANN BERNHARD

Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

公开(公告)号：SG185543A1

公开(公告)日：2012-12-28

申请号：SG2012083424

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  PIEPRZYK MARTIN

Inventor： JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

公开(公告)号：SG183029A1

公开(公告)日：2012-08-30

申请号：SG2012052031

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

Abstract: SINGLE-CELL NUCLEIC ACID ANALYSISThe present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.Fig. 1

公开(公告)号：SG172965A1

公开(公告)日：2011-08-29

申请号：SG2011050739

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.