公开(公告)号：ES2648313T3

公开(公告)日：2017-12-29

申请号：ES16157104

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： HANZEL DAVID ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID ,  MITSIS PAUL ,  CHRISTIANS FRED ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.

公开(公告)号：AU2006331512B2

公开(公告)日：2012-02-23

申请号：AU2006331512

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  OTTO GEOFFREY ,  HANZEL DAVID ,  PELUSO PAUL ,  TURNER STEPHEN ,  RANK DAVID ,  PHAM THANG

IPC: C12Q1/68 ,  C12N11/14

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

公开(公告)号：CA2633524A1

公开(公告)日：2007-07-05

申请号：CA2633524

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： BIBILLO AREK ,  CHRISTIANS FRED C ,  PARK INSIL ,  LYLE JOHN ,  OTTO GEOFF ,  PHAM THANG ,  RANK DAVID R ,  MURPHY DEVON ,  CLARK SONYA ,  MITSIS PAUL ,  PELUSO PAUL ,  HANZEL DAVID K

IPC: C12Q1/68

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：EP1963536A4

公开(公告)日：2010-03-03

申请号：EP06848948

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HANZEL DAVID K ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID R ,  MITSIS PAUL ,  CHRISTIANS FRED C ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C07K2319/20 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Y207/07007 ,  Y02P20/52

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：EP1963530A4

公开(公告)日：2009-08-05

申请号：EP06848008

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HANZEL DAVID ,  KORLACH JONAS ,  PELUSO PAUL ,  OTTO GEOFFREY ,  PHAM THANG ,  RANK DAVID ,  TURNER STEPHEN

IPC: C12Q1/68 ,  C12N11/14

CPC classification number: C12Q1/6837 ,  C07K2319/23 ,  C12N9/1252 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2565/531

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

公开(公告)号：EP1963501A4

公开(公告)日：2009-04-22

申请号：EP06848977

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HANZEL DAVID ,  KORLACH JONAS ,  PELUSO PAUL ,  OTTO GEOFF ,  PHAM THANG ,  RANK DAVID ,  TURNER STEPHEN

IPC: C12N11/00 ,  C12N11/16 ,  G01N33/53

CPC classification number: C12N9/22 ,  C07K2319/21 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2996 ,  Y10T428/31663 ,  Y10T428/31768

Abstract: Isolated and/or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and/or multiple surface coupling domains for surface coupling.