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    돼지 유행성 설사병 바이러스 진단용 재조합 항체 및 그 제조방법
    22.
    发明公开
    돼지 유행성 설사병 바이러스 진단용 재조합 항체 및 그 제조방법 无效
    一种用于诊断PIVCINE流行性腹泻病毒的重组抗体及其方法

    公开(公告)号：KR1020130055038A

    公开(公告)日：2013-05-28

    申请号：KR1020110118179

    申请日：2011-11-14

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 양동군 ,  오윤이 ,  조수동 ,  현방훈 ,  송재영 ,  안수환

    IPC: C12N15/866 ,  C12N7/01 ,  C07K16/08

    Abstract: PURPOSE: A recombinant antibody for diagnosing porcine epidemic diarrhea virus is provided to easily maintain or store an antibody producing strain. CONSTITUTION: A method for preparing a recombinant antibody for diagnosing porcine epidemic diarrhea virus comprises: a step of preparing a recombinant baculovirus vector containing a DNA fragment of sequence number 1; a step of transfecting baculovirus full length DNA Bac-N-Blue and the recombinant baculovirus vector into an insect cell and preparing recombinant baculovirus; and a step of culturing the recombinant baculovirus in the insect cell. The vector is pBlueBac4.5His. The insect cell is sf-9 cell.

    Abstract translation: 目的：提供用于诊断猪流行性腹泻病毒的重组抗体，以容易地维持或储存产生抗体的菌株。 构成：制备用于诊断猪流行性腹泻病毒的重组抗体的方法，包括：制备含有序列号1的DNA片段的重组杆状病毒载体的步骤; 将杆状病毒全长DNA Bac-N-Blue和重组杆状病毒载体转染入昆虫细胞并制备重组杆状病毒的步骤; 以及在昆虫细胞中培养重组杆状病毒的步骤。 载体是pBlueBac4.5His。 昆虫细胞是sf-9细胞。

    돼지 유행성설사병바이러스 중화 항체의 단쇄 가변 분절을 세균체 막표면에 발현하게 하는 벡터 및 돼지 유행성설사병 바이러스 증식을 억제하는 대장균
    26.
    发明公开
    돼지 유행성설사병바이러스 중화 항체의 단쇄 가변 분절을 세균체 막표면에 발현하게 하는 벡터 및 돼지 유행성설사병 바이러스 증식을 억제하는 대장균 有权
    转移载体，以表达单链抗变态反应片段，用于阻止细菌外膜上的PORCINE流行性腹膜病毒和E.COLI阻止PORCINE Epidemic DiarRHEA病毒的传播

    公开(公告)号：KR1020090011215A

    公开(公告)日：2009-02-02

    申请号：KR1020070074583

    申请日：2007-07-25

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 현방훈 ,  표현미 ,  김인중 ,  조수동 ,  김성희 ,  송재영

    IPC: C12N15/64 ,  C12N15/13 ,  C12N15/09

    Abstract: A vector and Escherichia coli suppressing proliferation of PED virus are provided to neutralize specifically the porcine epidemic diarrhea virus by being transformed to a surface expression vector and to reveal short chain variable segments of a porcine epidemic diarrhea virus neutralizing antibody at a film surface of a bacterial body. A surface expression vector reveals short chain variable segments of porcine epidemic diarrhea virus neutralizing antibody consisting of a sequence 1 at a film surface of a bacterial body. The surface expression vector includes a base sequence consisting of a sequence 1 and coding the short chain variable cleavage site specifically neutralized to porcine epidemic diarrhea virus(PEDV), T7 RNA polymerase consisting of a base sequence of 21bp~40bp of a sequence 4 and revealing the short chain variable split gene at a film surface of a bacterial body, a base sequence consisting of A sequence 2 coding A signal peptide of IgA proteinase gene of a gonococcus and an autotransporter beta domain gene consisting of a sequence 3 of the IgA proteinase of a base sequence coding six histidines and a gonococcus inserted into a bacterial membrane.

    Abstract translation: 提供抑制PED病毒增殖的载体和大肠杆菌，通过转化成表达表达载体来特异性中和猪流行性腹泻病毒，并显示在细菌膜表面的猪流行性腹泻病毒中和抗体的短链可变区段 身体。 表面表达载体揭示由细菌体的膜表面上的序列1组成的猪流行性腹泻病毒中和抗体的短链可变区段。 表面表达载体包括由序列1组成的碱基序列，并编码特异性中和到猪流行性腹泻病毒（PEDV）的短链可变切割位点，由序列4的21bp〜40bp的碱基序列组成的T7RNA聚合酶，并显示 在细菌体的膜表面的短链可变分裂基因，由编码淋球菌的IgA蛋白酶基因的A序列2的A序列组成的碱基序列和由IgA蛋白酶的序列3组成的自身转运蛋白β结构域基因 编码六个组氨酸的碱基序列和插入到细菌膜中的球菌属。

    돼지 유행성설사병 바이러스를 중화하는 특이 단클론 항체단쇄가변분절 유전자 및 이를 발현한 재조합단백질
    27.
    发明公开
    돼지 유행성설사병 바이러스를 중화하는 특이 단클론 항체단쇄가변분절 유전자 및 이를 발현한 재조합단백질 有权
    SCFV基因及其单克隆抗体2C10的重组蛋白中和病毒性腹膜病毒

    公开(公告)号：KR1020090011205A

    公开(公告)日：2009-02-02

    申请号：KR1020070074569

    申请日：2007-07-25

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 현방훈 ,  표현미 ,  김인중 ,  조수동 ,  김성희 ,  송재영 ,  권준헌

    IPC: C12N15/13 ,  C12N15/10 ,  C12N15/09

    Abstract: A gene comprising monoclonal antibody short chain variable segments neutralizing specifically porcine epidemic diarrhea virus(PEDV) is provided to prevent and treat PED pig disease. A gene comprising short chain variable segments neutralizes specifically porcine epidemic diarrhea virus(PEDV) consisting of a base sequence of a sequence 1. The gene comprising the short chain variable segments includes a heavy chain variable(VH) part consisting of a base sequence of 25~330 bp of the sequence 1 and a chain variable(VL) part consisting of a base sequence of 436~759 bp of the sequence 1. A monoclonal antibody includes a heavy chain variable(VH) part consisting of a base sequence of 25~330 bp of the sequence 1 and a chain variable(VL) part consisting of a base sequence of 436~759 bp of the sequence 1.

    Abstract translation: 提供包含中和特异性猪流行性腹泻病毒（PEDV）的单克隆抗体短链可变区的基因，以预防和治疗PED猪病。 包含短链可变区的基因特异性中和由序列1的碱基序列组成的猪流行性腹泻病毒（PEDV）。包含短链可变区的基因包括由25个碱基序列组成的重链可变（VH）部分 序列1〜330bp和由序列1的436〜759bp的碱基序列构成的链可变（VL）部分。单克隆抗体包括由25〜 序列1的330bp和由序列1的436〜759bp的碱基序列组成的链变量（VL）部分。

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