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公开(公告)号：KR1020040075385A
公开(公告)日：2004-08-30
申请号：KR1020030010830
申请日：2003-02-20
Applicant: 대한민국(농촌진흥청장)
IPC: A01H5/00
CPC classification number: C12N15/8274 , A01H4/005 , A01H4/008 , C12N5/0025 , C12N15/8205 , C12N15/8209 , C12N2500/34 , C12N2501/30
Abstract: PURPOSE: A method for producing transgenic perilla plants and herbicide-resistant perilla plants produced therefrom are provided, thereby easily inserting foreign useful genes into perilla plants, and reducing the production cost and environmental pollution. CONSTITUTION: The method for producing transgenic perilla plants comprises the steps of: (A) constructing an expression vector for transforming plants containing a foreign target gene and a selection maker; (B) introducing the expression vector into Agrobacterium to produce transformed Agrobacterium; (C) co-culturing the transformed Agrobacterium with a tissue section of perilla plants; (D) inducing shoots of the tissue section of perilla plants co-cultured in a selection-shoot inducing medium and subculturing the shoots of perilla plants in a root-inducing medium; and (F) transplanting the root-induced perilla plants in the field.
Abstract translation: 目的：提供一种生产转基因紫苏植物和由其生产的除草剂抗性紫苏植物的方法，从而容易地将外来的有用基因插入紫苏植物中，降低生产成本和环境污染。 构成：生产转基因紫苏植物的方法包括以下步骤：（A）构建用于转化含有外源靶基因的植物和选择标记物的表达载体; （B）将表达载体导入农杆菌以产生转化的土壤杆菌; （C）用紫苏植物的组织切片共转化转化的土壤杆菌; （D）诱导在选择性芽诱导培养基中共培养的紫苏植物的组织切片的芽，并在根诱导培养基中传代培养紫苏植物的芽; 和（F）在田间移植根诱导的紫苏植物。
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公开(公告)号：KR1020040066628A
公开(公告)日：2004-07-27
申请号：KR1020030003749
申请日：2003-01-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/29
CPC classification number: C07K14/415 , A61K35/74 , A61K38/168 , C12N15/70 , C12N15/8279 , C12Q1/6895
Abstract: PURPOSE: A gene which may be involved in signal transduction of disease resistance is provided, which can be useful for development of transgenic plants having disease resistance. CONSTITUTION: The gene which may be involved in signal transduction of disease resistance is DNA encoding a protein having 80% homology to the amino acid sequence set forth in SEQ ID NO: 1 or fragments thereof, wherein DNA has the nucleotide sequence set forth in SEQ ID NO: 2. An expression vector for inducing disease resistance of plants, pQE80L::(a,b,c) contains the gene encoding a protein having 80% homology to the amino acid sequence set forth in SEQ ID NO: 1. A microorganism transformed with the expression vector pQE80L::(a,b,c) is pQE80L:: a/E.coli CF1652(KACC 95010) or pQE80L::(a,b,c) is pQE80L:: b/E.coli CF1652(KACC 95011). A plant having disease resistance which is transformed with the expression vector pQE80L::(a,b,c) is provided.
Abstract translation: 目的：提供可能涉及抗病性信号转导的基因，可用于开发具有抗病性的转基因植物。 构成：可能涉及抗病性信号转导的基因是编码与SEQ ID NO：1所示的氨基酸序列或其片段具有80％同源性的蛋白质的DNA，其中DNA具有SEQ所示的核苷酸序列 编号：2.用于诱导植物抗病性的表达载体pQE80L：:( a，b，c）含有编码与SEQ ID NO：1所示的氨基酸序列具有80％同源性的蛋白质的基因。 表达载体pQE80L转化的微生物:( a，b，c）是pQE80L :: a /大肠杆菌CF1652（KACC 95010）或pQE80L：:( a，b，c）是pQE80L :: b / E.coli CF1652（KACC 95011）。 提供了用表达载体pQE80L转化的具有抗病性的植物:( :( a，b，c））。
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公开(公告)号：KR1019970011556B1
公开(公告)日：1997-07-12
申请号：KR1019930028570
申请日：1993-12-20
Applicant: 대한민국(농촌진흥청장)
Abstract: Extracts and its crude polysaccharides from Formitella fraxinea useful as anticancer agent, immunity enhancer and functional foods are described. Extracts obtained from title mushroom in hot water is centrifuged and filtered. Ethanol is added 2 times by volume to filtrate and precipitates is formed. Precipitates is suspension-washed and freeze-dried to obtain crude polysaccharide composed of glucose : galactose : mannose : xylose : fucose : ribose at the weight ratio of 100 : 50 : 54 : 7 : 14 : 13. Above crude polysaccharides are separated by anion exchange resin column chromatography to obtain neutral polysaccharides and acidic polysaccharides having a different weight ratio.
Abstract translation: 描述了提取物及其作为抗癌剂，免疫增强剂和功能性食品的来自Formitella fraxinea的粗多糖。 从热水中的标题蘑菇获得的提取物被离心并过滤。 向滤液中加入2倍体积的乙醇，形成沉淀。 将沉淀物悬浮洗涤并冷冻干燥，得到由葡萄糖组成的粗多糖：半乳糖：甘露糖：木糖：岩藻糖：核糖，重量比为100:50:54:7:14:13。粗多糖由阴离子 交换树脂柱色谱以获得具有不同重量比的中性多糖和酸性多糖。
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公开(公告)号：KR1019950018456A
公开(公告)日：1995-07-22
申请号：KR1019930030716
申请日：1993-12-29
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 식물이 외부 병원균의 침입을 받는 때에만 발현되는 소위 유도성 유전자의 조절부위를 제공한다.
본 발명의 유도성 유전자의 조절부위는 토마토의 페닐알라닌암모니아 라이아제 유전자, 즉 PAL5 유전자의 조절부위로서 다음의 염기서열(KFCC-10812)을 갖는다:
GTTATTTCTTTCGTAGCAACTAATATAAAATTGATGACATATTCA
TATAATAATATTAAATAATATAGAAAAGCTGCAAATTAATTAGAT -1081
TTTATTTGTGTGAATTTGTTCAAATTATTAGGTTGAGATCCAAAT
ATAATTAAAAGAGACGAATTATGTTAGATTGACCAATTAAAGTACA -991
ACGGATTAACAATAATATTCATAGTTATTCTTATTTTTTTAATCG
TAGTTGATTAATTCTTGATTGAAACTAATTTCATAGTAAAAAAAT -901
ATTAAACTTTAAATTTCTGTGATAAACATTTTCACTTATTGAAAA
TAGTAATACAAATATAAAAACACTCCTAATAAATAATAAACTAT -881
AGGATTTAAACCGATAAAATAAATATCTACTTCTATTTGTAAATG
TTTTTGTACTAGTAATTACAAAGTATATCATACTTTTTCTATAAA -721
TTAGTTTAAAGCGTATGTATGTCTTAGTGTTTTTTATACGATAAA
AATAAATTAAGAGACACGAAAGTAAGACATACACATTGATAATC -631
ACTAAATCAATATTTAATAATTCTATAATAATTTAGCATGTCTAC
AAACTGATAGCCGATAAATCAAATCAAAATGACCGAATATTCAAC -541
CCTAATATTAGAAATTTTTATCTATGTTAATAATCATTTACGTCT
CTCACCAACATTAAGAAGAAAAGAGTGGTAGAATTACAATTTAAC -451
CCTTTTATTTTCTCCCAACAACCCAAAAAAAACTCCAAACATATC
CTAAATCCACCAACCCCCAAAATTTAATTTTTTTTTCTCTCAATA -361
TATTTTTAAAAAAAATTATATCCATGTTGCATGAAATACCCACGT
CATCCTCAAATCTCAACCGTTAATCGCAATTAAAATCAATGGCTA -271
TAATTAATCTTCCAACAACCACCATTTTTAGTCATTTCCTACAACCCCC
TCTCACATAATTTTCTTTACCTACCATCCTTTGTTCCTCTC -181
TATATATACTCACCACATATATCATCTACCATAACCAAAAAAAAT
AATAATAATACACTAATCATAGTTCACAACATATTTTTTTTTATA -91
TATATAAATAAAATTCCCATTTTTTCTCTTCTCCAAATTCTCCTAA
AGTAAAATTTCTCCATTACAAATCAAACCATTTTTTGTTGGTCCA -1
본 발명은 또한 상가 유전자 단편(PAL5의 조절부위)과 표식인자인 GUS(β-glucuronidase) 유전자를 함께 결합한 후 식물 형질전환용 벡터를 이용하여 식물에 도입하므로써 형질전환된 개개의 식물체로부터 병원균 침입시 유도되는 발현양을 조사할 수 있을 뿐만 아니라 식물의 내병성 유전자와 상기 유전자 단편을 결합한 후 식물에 도입하므로써 유전적으로 내병성을 갖는 식물을 만들어 낼 수 있다.-
公开(公告)号：KR1019950016536A
公开(公告)日：1995-07-20
申请号：KR1019940004134
申请日：1994-03-03
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 한국간 꿩의다리속(Thalictrum Linne)으로 부터 분리한 종자유에 관한 것이다. 본 발명의 한국산 꿩의다리속으로 부터 분리한 종자유는 지방산 조성에 있어서, 총지방산에 대하여 54.5%의 조성물을 가지므로, 종래 가장 리놀레산을 다량 함유한 것으로 보고된 다른 꿩의다리속 종자유에 함유된 40.1% 내지 46.2%의 △
5 -트랜스-리놀레산 보다도 약 10% 정도 더 많이 함유한 것으로 밝혀졌다. 한국산 꿩의다리속으로 부터 분리된 종자유는 여러가지 기능성 식품 및 약물로 사용하는 것이 가능할 것이다.-
Patent Agency Ranking
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公开(公告)号：KR1019740000271B1
公开(公告)日：1974-09-20
申请号：KR1019730002116
申请日：1973-12-12
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
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公开(公告)号：KR1019970011310B1
公开(公告)日：1997-07-09
申请号：KR1019930028158
申请日：1993-12-17
Applicant: 대한민국(농촌진흥청장)
Abstract: The diagnostic method is disclosed which makes it possible to determine the presence of a virus infection in a crop by using the primer for nucleic acid amplification. The method provides the primer which can assay virus related diseases specifically, which is used to characteristic amplifying a specific DNA sequence. Included in the invention are the extraction of virus RNA from virus infected plant, the synthesis of cDNA of single strand from the virus RNA by means of reverse transcription reaction with using the primer, polymerase chain reaction(PCR) by using the primer for the template of the cDNA, and the certification of a virus specific DNA fragment band by agarose gel electrophoresis of the PCR product.
Abstract translation: 公开了诊断方法，其可以通过使用用于核酸扩增的引物来确定作物中病毒感染的存在。 该方法提供了可以特异性测定病毒相关疾病的引物，其用于特异性扩增特异性DNA序列。 本发明包括从病毒感染的植物中提取病毒RNA，通过使用引物的逆转录反应合成来自病毒RNA的单链cDNA，使用引物作为模板进行聚合酶链式反应（PCR） 的cDNA，通过PCR产物的琼脂糖凝胶电泳鉴定病毒特异性DNA片段。
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公开(公告)号：KR1019970004942B1
公开(公告)日：1997-04-10
申请号：KR1019930030716
申请日：1993-12-29
Applicant: 대한민국(농촌진흥청장)
Abstract: The control part of a inductive protective gene is extracted from young leaves in tomato, and phenylalanine ammonia lyase(PAL) of the above tomato is recombined with a disease resistant structural gene so that it is revealed only when virus invades therein.
Abstract translation: 诱导性保护基因的对照部分是从番茄中的幼叶提取的，上述番茄的苯丙氨酸氨裂解酶（PAL）与抗病结构基因重组，只有当病毒侵入时，才能发现。
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公开(公告)号：KR1019970000686B1
公开(公告)日：1997-01-18
申请号：KR1019930030715
申请日：1993-12-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/82
Abstract: The chloroplast autoreplicating sequence (ARS) was isolated from a chloroplast of rape. A vector containing the ARS is prepared by recombining a selection marker and a promotor sequence with the said ARS. pECTRHG vector used for transforming plants or bacteria was prepared by a recombination of a replicative origin of E. coli plasmid, pUC19 having ampicillin resistant gene as the selection factor of the plant cell, and PrbcLp and PatpB as a promotor for expression in a plant microorgan.
Abstract translation: 从强奸的叶绿体中分离叶绿体自动重复序列（ARS）。 通过将选择标记和启动子序列与所述ARS重组来制备含有ARS的载体。 用于转化植物或细菌的pECTRHG载体通过重组大肠杆菌质粒的复制起点，具有氨苄青霉素抗性基因的pUC19作为植物细胞的选择因子，PrbcLp和PatpB作为在植物微生物中表达的启动子来制备 。
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