公开(公告)号：SG185544A1

公开(公告)日：2012-12-28

申请号：SG2012083432

申请日：2011-05-16

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  ZIMMERMANN BERNHARD

Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

公开(公告)号：SG185543A1

公开(公告)日：2012-12-28

申请号：SG2012083424

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  PIEPRZYK MARTIN

Inventor： JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  PIEPRZYK MARTIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

公开(公告)号：EP2326732A4

公开(公告)日：2012-11-14

申请号：EP09812052

申请日：2009-08-26

Applicant: FLUIDIGM CORP

Inventor： MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  UNGER MARC ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6853 ,  Y10T436/143333 ,  C12Q2537/143 ,  C12Q2525/161

公开(公告)号：CA2734868C

公开(公告)日：2019-09-10

申请号：CA2734868

申请日：2009-08-26

Applicant: FLUIDIGM CORP

Inventor： MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  UNGER MARC ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6848 ,  C40B70/00

Abstract: The present disclosure relates to methods for detecting a plurality of target nucleic acids in a plurality of samples comprising providing S samples that will be mixed together prior to assay, where S is an integer greater than 1; separately subjecting each of said S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence comprising a sample-specific nucleotide tag and a target nucleotide sequence; wherein T is the number of target nucleic acids to be detected, T being an integer greater than one; mixing together tagged target nucleotide sequences from said S samples to form an assay mixture; dividing the assay mixture into up to S × T amplification mixtures, and separately subjecting each of said amplification mixtures to amplification using a unique pair of amplification primers.

公开(公告)号：CA2698545C

公开(公告)日：2014-07-08

申请号：CA2698545

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: C12Q1/68

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

公开(公告)号：AU2008295992B2

公开(公告)日：2014-04-17

申请号：AU2008295992

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: G01N33/48

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

公开(公告)号：ES2380844T3

公开(公告)日：2012-05-18

申请号：ES08829141

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: C12Q1/68

Abstract: Un método para determinar el número de copias relativo de una secuencia polinucleotídica diana en el genoma de un sujeto, que comprende: preamplificar una secuencia polinucleotídica diana y una secuencia polinucleotídica de referencia en una muestra que contiene ADN genómico del sujeto; ensayar la secuencia polinucleotídica diana y la secuencia polinucleotídica de referencia de la muestra preamplificada mediante una PCR digital; determinar (a) el número de moléculas polinucleotídicas amplificadas que contienen la secuencia polinucleotídica diana, y (b) el número de moléculas polinucleotídicas amplificadas que contienen la secuencia polinucleotídica de referencia, y determinar la proporción de (a) a (b).

公开(公告)号：MX2010002556A

公开(公告)日：2010-08-02

申请号：MX2010002556

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: G01N33/48

Abstract: La presente invención es concerniente con métodos y sistemas para determinar la variación del número de copia de un polinucleótido objetivo en un genoma de un sujeto, que incluye técnicas a base de amplificación. Los métodos pueden incluir preamplificación de la muestra seguida por distribución de muestra y una pluralidad de volúmenes de reacción, detección cuantitativa de un polinucleótido objetivo y un polinucleótido de referencia y análisis para determinar el número de copia relativo de la secuencia de polinucleotido objetivo en el genoma del sujeto.