公开(公告)号：CA2738626C

公开(公告)日：2017-08-08

申请号：CA2738626

申请日：2009-09-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZACCARIN DENIS ,  LUNDQUIST PAUL ,  ZHAO PEIQIAN ,  ZHONG FRANK ,  TURNER STEPHEN ,  HUANG YANQIAO ,  MONADGEMI PEZHMAN ,  SAXENA RAVI ,  GROT ANNETTE ,  RULISON AARON

IPC: G02B6/12 ,  G01N21/00

Abstract: Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.

公开(公告)号：CA2645758C

公开(公告)日：2016-08-09

申请号：CA2645758

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：AU2010245304B2

公开(公告)日：2015-06-04

申请号：AU2010245304

申请日：2010-04-26

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  SCHADT ERIC

IPC: C12N15/10 ,  C12Q1/68

Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance or inhibit such reactions. In certain preferred embodiments, RNA templates are used in single-molecule real-time sequencing reactions.

公开(公告)号：AU2009325069B2

公开(公告)日：2015-03-19

申请号：AU2009325069

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2009300369B2

公开(公告)日：2014-08-14

申请号：AU2009300369

申请日：2009-09-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZACCARIN DENIS ,  LUNDQUIST PAUL ,  ZHAO PEIQIAN ,  ZHONG FRANK ,  TURNER STEPHEN ,  HUANG YANQIAO ,  MONADGEMI PEZHMAN ,  SAXENA RAVI ,  GROT ANNETTE ,  RULISON AARON

IPC: G01N21/00 ,  G02B6/12

Abstract: Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination.

公开(公告)号：AU2007215242B2

公开(公告)日：2012-10-18

申请号：AU2007215242

申请日：2007-02-09

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： ZACCARIN DENIS ,  FOQUET MATHIEU ,  MAXHAM MARK ,  TURNER STEPHEN ,  LUNDQUIST PAUL ,  LACROIX YVES

IPC: F21V9/16 ,  G01J1/58 ,  G01T1/10 ,  G21H3/02 ,  G21K5/00

Abstract: Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.

公开(公告)号：AU2006331677A1

公开(公告)日：2007-07-05

申请号：AU2006331677

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： RANK DAVID ,  KORLACH JONAS ,  HANZEL DAVID ,  PHAM THANG ,  OTTO GEOFF ,  TURNER STEPHEN ,  PELUSO PAUL

IPC: C12N11/00 ,  C12N11/16 ,  G01N33/53

公开(公告)号：CA2991818C

公开(公告)日：2022-10-11

申请号：CA2991818

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: Compositions for nucleic acid sequencing are provided that include template constructs that comprise double stranded portions in partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.

公开(公告)号：CA2790393C

公开(公告)日：2019-03-12

申请号：CA2790393

申请日：2011-02-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MCCAFFREY NATHANIEL JOSEPH ,  TURNER STEPHEN ,  SAXENA RAVI ,  HELGESEN SCOTT EDWARD

IPC: G01N21/64 ,  B82Y15/00 ,  G01N21/01 ,  G01N21/11 ,  G01N21/63 ,  G08C23/04

Abstract: An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.

公开(公告)号：AU2015202111B2

公开(公告)日：2017-03-16

申请号：AU2015202111

申请日：2015-04-24

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/68