Abstract:
It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucleic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.
Abstract:
A cardiac arrest fluid which contains trehalose and metal ions. The content of trehalose is preferably 3.5% (w/v) or lower, or 1.5 to 3.5% (w/v). The metal ions are preferably Na?+, K+, Mg++¿, and Ca++. The cardiac arrest fluid is useful for cardiac arrest and heart muscle protection.
Abstract:
An antibody binding specifically to rat postacrosome reaction sperm is produced and hybridomas (FARS-91 and FARS-92 strains) capable of stably proliferating are obtained by fusing mouse spleen cells having a high antibody titer against rat postacrosome reaction sperm with mouse-origin myeloma cells and screening fused cells reacting strongly with rat postacrosome reaction sperm. From these hybridomas, monoclonal antibodies selectively binding to rat postacrosome reaction sperm can be obtained. Thus, a diagnostic method for evaluating rat fertility is presented.
Abstract:
A method for evaluating renal function by assaying megsin protein in a biological sample; and an immunoassay reagent and kit which are useful in assaying megsin protein in a biological sample.
Abstract:
A composition promoting lacrimal secretion which can be safely and efficaciously used in the treatment of promoting lacrimal secretion different from the conventional treatment of supplying lacrimal components. This composition is characterized by containing a component activating PAR-2. Also, contact lenses carrying and/or containing this composition are provided.
Abstract:
A microorganism causative of an infection is quickly and highly sensitively detected and/or identified by obtaining phagocytes from a clinical specimen containing phagocytes originating in a living body, immobilizing the obtained phagocytes, treating the phagocytes so as to enhance the permeability of the phagocyte membrane and expose DNA of the microorganism causative of the infection which is anticipated as being contained in the phagocytes, and then using a detection DNA probe hybridizable with the DNA under stringent conditions.