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公开(公告)号：KR1020140117856A
公开(公告)日：2014-10-08
申请号：KR1020130032688
申请日：2013-03-27
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to a method for flocculating microalgae, and the present invention provides the method for flocculating microalgae using a method which is economical and easy to be operated. The microalgae of the present invention can be used as useful biomass for biodiesel production, and acquired microalgae, as an alternative energy, provides a sustainable resource which has no concern for exhaustion.
Abstract translation: 本发明涉及一种絮凝微藻的方法，本发明提供一种使用经济且易于操作的方法絮凝微藻的方法。 本发明的微藻可用作用于生物柴油生产的有用生物质，作为替代能源的获得性微藻提供了不关心耗尽的可持续资源。
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32.发明公开(a) 불활성화 락테이트 디하이드로제나제 및 (b) 불활성화 수크로즈 조절자를 포함하는 2,3-부탄다이올 생성용 재조합 균주 有权用于制造包含灭活的乳酸脱氢酶和灭活的SUCROSE调节剂的2,3-丁二醇的重组菌
公开(公告)号：KR1020140095945A
公开(公告)日：2014-08-04
申请号：KR1020130083817
申请日：2013-07-16
Applicant: 서강대학교산학협력단
CPC classification number: C12P7/18 , C07K14/245 , C07K14/26 , C12N9/0006 , C12N15/00 , C12Y101/01027
Abstract: The present invention relates to a recombinant strain producing 2,3-butanediol which comprises (a) an inactivated lactate dehydrogenase and (b) an inactivated sucrose regulator. According to the present invention, 2,3-butanediol is economically produced using a cheap carbon source in the present invention, and excellent 2,3-butanediol efficiency and productivity are obtained compared with a wild type.
Abstract translation: 本发明涉及产生2,3-丁二醇的重组菌株，其包含（a）灭活的乳酸脱氢酶和（b）灭活的蔗糖调节剂。 根据本发明，与本发明相比，本发明中使用廉价碳源经济地制造2,3-丁二醇，与野生型相比，获得了优异的2,3-丁二醇效率和生产率。
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公开(公告)号：KR1020140052156A
公开(公告)日：2014-05-07
申请号：KR1020120116959
申请日：2012-10-19
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to a transformed yeast for improving a fatty acid content and a method for producing same. Provided is a transformed yeast for producing transformed fatty acid through a recombinant vector comprising: (i) a nucleotide sequence that codes oxoacyl carrier protein synthetic enzyme II (fabF) that is derived from Saccharomyces cerevisiae; (ii) a nucleotide sequence that codes oxoacyl carrier protein reduced enzyme (fabG) that is derived from Streptococcus pyogenes; (iii) a nucleotide sequence that codes hydroxyl myristoyl carrier protein dehydrated enzyme (fabZ) that is derived from Streptococcus pyogenes; (iv) a nucleotide sequence that codes ynoyl carrier protein reduced enzyme II (fabK) that is derived from Streptococcus pyogenes; or (v) a nucleotide sequence that codes oleyl carrier protein dehydrated enzyme (EC 3.1.2.14) that is derived from Streptococcus pyogenes. In the present invention, by means of Saccharomyces cerevisiae, a fatty acid biosynthesis path extending step is over-expressed so that the content of fatty acid can be increased. Through the over-expression from glucose to a fatty acid biosynthesis path and changes in a metabolism flow by means of the insertion of a gene of a different kind, fatty acid biosynthesis can be effectively mass produced.
Abstract translation: 本发明涉及一种改善脂肪酸含量的转化酵母及其制备方法。 提供了通过重组载体产生转化的脂肪酸的转化酵母，其包含：（i）编码来源于酿酒酵母的氧代酰基载体蛋白质合成酶II（fabF）的核苷酸序列; （ii）编码源自化脓性链球菌的氧代酰基载体蛋白质还原酶（fabG）的核苷酸序列; （iii）编码衍生自化脓性链球菌的羟基肉豆蔻酰载体蛋白脱水酶（fabZ）的核苷酸序列; （iv）编码衍生自化脓性链球菌的甲酰基载体蛋白质还原酶II（fabK）的核苷酸序列; 或（v）编码来源于化脓性链球菌的油基载体蛋白脱水酶（EC 3.1.2.14）的核苷酸序列。 在本发明中，通过酿酒酵母，脂肪酸生物合成途径延伸步骤被过度表达，从而可以增加脂肪酸的含量。 通过从葡萄糖到脂肪酸生物合成途径的过度表达，通过插入不同种类的基因来改变代谢流，可以有效地大量生产脂肪酸生物合成。
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公开(公告)号：KR1020140026912A
公开(公告)日：2014-03-06
申请号：KR1020120092750
申请日：2012-08-24
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to a method for preparing transformed Escherichia coli for expressing acyl-′acyl carrier protein′ thioesterase which includes the steps of: (a) obtaining a nucleotide sequence of first sequence in sequencing list by codon-optimization of a nucleotide sequence which codes Streptococcus pyogenes-derived acyl-′acyl carrier protein′ thioesterase in order to be optimized to be expressed in the Escherichia coli; (b) preparing a recombinant vector by inserting the codon-optimized nucleotide sequence of the acyl-′acyl carrier protein′ thioesterase; and (c) preparing recombinant Escherichia coli by transforming the recombinant vector into the Escherichia coli. The method can produce a large amount of fatty acids which will become the base of bioenergy production, are environmentally friendly, and have great financial power by increasing the production of saturated fatty acids of C12:0 and C14:0 and unsaturated fatty acids of C18:1 among fatty acids produced from Escherichia coli by 200-400% compared with the production by a wild type.
Abstract translation: 本发明涉及一种用于表达酰基 - 酰基载体蛋白'硫酯酶的转化大肠杆菌的制备方法，其包括以下步骤：（a）通过核苷酸序列的密码子优化获得测序列表中第一序列的核苷酸序列， 代谢化脓性链球菌衍生的酰基 - 酰基载体蛋白'硫酯酶，以优化在大肠杆菌中表达; （b）通过插入酰基 - 酰基 - 载体蛋白'硫酯酶的密码子优化的核苷酸序列来制备重组载体; 和（c）通过将重组载体转化到大肠杆菌中制备重组大肠杆菌。 该方法可以产生大量的脂肪酸，成为生物能源生产的基础，环保，通过增加C12：0和C14：0的饱和脂肪酸和C18不饱和脂肪酸的生产具有巨大的财力 ：大肠杆菌生产的脂肪酸中的1种与野生型相比，产量为200-400％。
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公开(公告)号：KR101351989B1
公开(公告)日：2014-01-27
申请号：KR1020120015593
申请日：2012-02-16
Applicant: 서강대학교산학협력단
Abstract: 본 발명은 알코올 디히드로제나아제(alcohol dehydrogenase) 1, 알코올디히드로제나아제 3 및 알코올 디히드로제나아제 5로 구성된 군으로부터 선택되는 하나이상의 알코올 디히드로제나아제를 불활성화시키는 단계를 포함하는 2,3-부탄다이올 제조용 효모의 제조방법에 관한 것이다. 본 발명은 화학공업에 유용한 기초가 될 플랫폼용 화합물인 2,3-부탄다이올 제조용 효모 제조방법, 2,3-부탄다이올 제조용 효모 및 2,3-부탄다이올을 제공할 수 있으며, 본 발명의 2,3-부탄다이올 제조용 효모는 야생형 균주와 비교하여 50-100배 향상된 우수한 2,3-부탄다이올 효율을 나타낸다.
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Patent Agency Ranking
公开(公告)号：KR101334971B1
公开(公告)日：2013-12-05
申请号：KR1020110022359
申请日：2011-03-14
Applicant: 서강대학교산학협력단
CPC classification number: C12P7/16 , C12N9/0006 , C12N9/88 , C12Y101/01001 , C12Y101/01004 , C12Y401/01005 , Y02E50/10
Abstract: 본 발명은 (a) 아세토락테이트 디카복실실레이즈(acetolactate decarboxylase)를 코딩하는 뉴클레오티드; 및 (b) 알코올 디하이드로지네이즈(alcohol dehydrogenase)를 코딩하는 뉴클레오티드로 구성된 군으로부터 선택되는 최소 1종의 뉴클레오티드 서열을 포함하는 발현벡터로 형질전환된(cotransformed) 2,3-부탄다이올 과발현용 대장균을 제공한다. 본 발명의 형질전환 대장균은 야생종 크렙시엘라 뉴모니아 KCTC 2242와 비교하였을 때, 포도당의 소모량에 따른 2,3-부탄다이올의 생산량이 20% 증가하였다.
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公开(公告)号：KR101334973B1
公开(公告)日：2013-12-02
申请号：KR1020110061249
申请日：2011-06-23
Applicant: 서강대학교산학협력단
Abstract: 본 발명은 지방산 과발현용 형질전환 대장균의 제조방법에 관한 것으로, 보다 상세하게는, (a)
fabB (3-옥소아실-[아실-전송-단백질] 신타아제Ⅰ),
fabG (3-옥소아실-[아실-전송-단백질] 리덕타아제),
fabZ (3R-하이드록시미리스톨 아실 전송 단백질 디하이드레타아제) 또는
fabI (이노일-[아실-전송-단백질] 리덕타아제)를 코딩하는 뉴클레오타이드 서열로 구성된 군으로부터 선택되는 하나 이상의 뉴클레오타이드 서열을 발현 벡터에 삽입시키는 단계; 및 (b) 상기
fabB ,
fabG ,
fabZ 및
fabI 를 코딩하는 뉴클레오타이드가 삽입된 발현벡터로 대장균을 형질전환 시키는 단계에 관한 것이다. 본 발명에 따르면, 본 발명은 지방산 생합성 경로의 과발현용 형질전환 대장균의 제조방법을 제공할 수 있으며, 글루코오즈를 하이드로카본으로 전환시키는 지방산 신장 단계의 재조합 대장균을 제공한다. 또한 본 발명은 하나 이상의 유전자 조합에 따라 미생물의 지방산 생합성 대사산물의 생산을 증대 할 수 있다.-
公开(公告)号：KR1020130117907A
公开(公告)日：2013-10-29
申请号：KR1020120039121
申请日：2012-04-16
Applicant: 서강대학교산학협력단 , 고려대학교 산학협력단
Abstract: PURPOSE: A method for preparing transformed E. coli for expressing phosphoenolpyruvate carboxylase (PEPC) is provided to obtain a large amount of PEPC, to improve the activity of PEPC, and to produce industrially useful materials. CONSTITUTION: A method for preparing transformed E. coli for expressing PEPC comprises the steps of: acquiring a nucleotide sequence of sequence number 1 of PEPC of Glaciecola sp. by codon optimization for expressing the nucleotide sequence in E. coli; inserting the codon-optimized nucleotide sequence into an expression vector and preparing a recombinant vector; and transducing the recombinant vector to E. coli and preparing transformed E. coli.
Abstract translation: 目的：提供用于表达磷酸烯醇丙酮酸羧化酶（PEPC）的转化大肠杆菌的制备方法，以获得大量的PEPC，提高PEPC的活性，并生产工业上有用的物质。 构成：用于制备用于表达PEPC的转化的大肠杆菌的方法包括以下步骤：获得Glaciecola sp。的PEPC序列号1的核苷酸序列。 通过密码子优化在大肠杆菌中表达核苷酸序列; 将密码子优化的核苷酸序列插入表达载体并制备重组载体; 并将重组载体转导到大肠杆菌中并制备转化的大肠杆菌。
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公开(公告)号：KR1020130095370A
公开(公告)日：2013-08-28
申请号：KR1020120016727
申请日：2012-02-20
Applicant: 서강대학교산학협력단
Abstract: PURPOSE: A Klebsiella mutant strain for producing 2,3-butanediol is provided to stably produce 2,3-butanediol with the similar growth rate with a wild type. CONSTITUTION: A Klebsiella mutant strain has inactivated wabG gene and is apathogenic. The strain has similar 2,3-butanediol productivity with a wild type containing wabG gene. Klebsiella is Klebsiella pneumoniase, Klebsiella oxytoca, Klebsiella granulomatis, or Klebsiella terigena. The mutant strain does not have capsule polysaccharides. [Reference numerals] (A) Wild type Klebsiella pneumoniae 2242; (B) Mutant type Klebsiella pneumoniae 2242
Abstract translation: 目的：提供用于生产2,3-丁二醇的克雷伯氏菌突变菌株，以稳定生产具有与野生型相似生长速率的2,3-丁二醇。 构成：Klebsiella突变株具有灭活的wabG基因，是致病性的。 该菌株具有与含有wabG基因的野生型相似的2,3-丁二醇生产力。 克雷伯菌是克雷伯菌肺炎球菌，克雷伯氏杆菌，肉芽肿克雷伯杆菌或特雷霉杆菌。 突变株不含胶囊多糖。 （A）野生型肺炎克雷伯菌2242; （B）突变型肺炎克雷伯菌2242
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公开(公告)号：KR1020130058236A
公开(公告)日：2013-06-04
申请号：KR1020110124138
申请日：2011-11-25
Applicant: 서강대학교산학협력단
CPC classification number: C12N9/0006 , C12N15/70 , C12P7/18 , C12Y101/01004
Abstract: PURPOSE: A method for preparing meso-2,3-butandiol from acetoin using a recombinant microorganism containing a novel acetoin reductase is provided to obtain a large amount of compounds for a platform. CONSTITUTION: Acetoin reductase has an amino acid sequence of sequence number 2. A nucleic acid molecule encodes acetoin reductase and has a nucleotide sequence of sequence number 1. A vector contains the nucleic acid molecule. A microorganism is transformed by the nucleic acid molecule. The transformed microorganism is transformed E.coli. A method for preparing 2,3-butanediol comprises a step of culturing the transformed microorganism.
Abstract translation: 目的：提供使用含有新型乙偶姻还原酶的重组微生物从乙偶姻制备内消旋-2,3-丁二醇的方法，以获得大量的平台化合物。 构成：乙酰辅酶还原酶具有序列号2的氨基酸序列。核酸分子编码乙偶姻还原酶并具有序列号1的核苷酸序列。载体含有核酸分子。 微生物被核酸分子转化。 转化的微生物转化大肠杆菌。 制备2,3-丁二醇的方法包括培养转化的微生物的步骤。
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