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31.发明授权중합효소연쇄반응 및 제한효소 절편길이 다형성을 이용하여 국화황화모틀바이로이드의 변이주를 구별하는 방법 有权使用聚合酶链反应 - 限制性片段长度多态性对菊花褪绿斑马疹病毒株进行菌株鉴别的方法
公开(公告)号：KR101402464B1
公开(公告)日：2014-06-03
申请号：KR1020120115652
申请日：2012-10-17
Applicant: 성균관대학교산학협력단
Abstract: 본 발명은 국화황화모틀바이로이드(Chrysanthemum chlorotic mottle viroids, CChMVd)의 변이주(Strain)를 중합효소연쇄반응 및 제한효소 절편길이 다형성(Polymerase chain reaction-Restriction fragment length polymorphism, PCR-RFLP)법을 이용하여 구별하는 방법에 관한 것이다. 본 발명에 따른 방법을 이용하면 효과적으로 국화황화모틀바이로이드의 5개 변이주인 msim34, SSHA6, Beijing, India 669541, India 646404를 구별할 수 있다. 따라서 국화재배농가에 막대한 피해를 주고 있는 국화 감염성 바이로이드를 조기에 신속하고 정확하게 검출 가능하게 하여, 막대한 경제 손실을 현저히 감소시킬 수 있을 것으로 기대된다.
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32.发明公开국화왜화바이로이드를 검출하기 위한 등온증폭 반응용 프라이머 조성물, 및 이의 용도 无效用于循环介导的同位素放大反应以检测CHRYSANTHEMUM STUNV VIROID的PRIMER组合物及其用途
公开(公告)号：KR1020140048002A
公开(公告)日：2014-04-23
申请号：KR1020120114456
申请日：2012-10-15
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q1/04 , C12Q2527/101
Abstract: The present invention relates to a primer set for an isothermal amplification reaction to detect Chrysanthemum stunt viroid (CSVd), a composition comprising the primer set, and a method for detecting CSVd using the composition. When the primer set for an isothermal amplification reaction to detect CSVd according to the present invention is used, the CSVd can be effectively detected without technical equipment on the spot for a short period of time. In addition, viroids can be detected even with a small amount of sample, and the diagnostic results can be promptly verified by the naked eye under the natural light by using a high-concentration of SYBR Green I. Therefore, Chrysanthemum infectious viroids that seriously damage Chrysanthemum farmhouses can be detected early, promptly, and accurately, thereby significantly reducing a huge economic loss due to the Chrysanthemum infectious viroids.
Abstract translation: 本发明涉及用于检测菊花特发性类病毒（CSVd）的等温扩增反应的引物组，包含引物组的组合物，以及使用该组合物检测CSVd的方法。 当使用根据本发明的用于检测CSVd的等温扩增反应的引物时，可以在没有技术设备的情况下，在现场短时间内有效地检测CSVd。 此外，即使使用少量的样品也能够检测到病毒，并且通过使用高浓度的SYBR Green I，可以在自然光下通过肉眼迅速验证诊断结果。因此，严重损害菊花感染性病毒 可以及早，准确地检测到菊花农舍，从而大大减少了菊花感染性病毒的巨大经济损失。
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33.发明公开국내 토마토황화잎말림바이러스의 ＤＮＡ-Ａ 유전자 및 무늬인동황화잎맥모자이크바이러스의 베타 위성 ＤＮＡ 유전자, 및 이를 이용한 식물체 감염시스템 有权TOMATO黄叶病毒的病毒基因组序列病毒分离株KOREA ISOLATE DNA-A和HONEYSUCKLE黄色VEIN MOSAIC VIRUS KOREA分离的BETA-SATELLITE DNA和感染系统到使用该植物的植物
公开(公告)号：KR1020130123669A
公开(公告)日：2013-11-13
申请号：KR1020120046970
申请日：2012-05-03
Applicant: 성균관대학교산학협력단
Abstract: The present invention relates to viral genome sequences of tomato yellow leaf curl virus Korea isolate DNA-A inducing leaf curling, honeysuckle yellow vein mosaic virus Korea isolate beta-satellite DNA, and a recombinant plasmid including the same. The present invention can induce virus infection in various plant bodies and can be applied to various studies including the prevention of virus by using the various plant bodies.
Abstract translation: 本发明涉及番茄黄叶卷曲病毒韩国分离株DNA-A诱导叶卷曲，金银花黄色静脉花叶病毒韩国分离株β卫星DNA的病毒基因组序列及其重组质粒。 本发明可以诱导各种植物体内的病毒感染，可以应用于各种研究，包括通过使用各种植物体来预防病毒。
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公开(公告)号：KR1020130117165A
公开(公告)日：2013-10-25
申请号：KR1020120040020
申请日：2012-04-17
Applicant: 성균관대학교산학협력단
IPC: A01K67/027
CPC classification number: A01K67/033 , A01K2207/30
Abstract: PURPOSE: An acquired lymphedema animal model and a method for producing the same are provided to form bigger edema than the lymphedema animal and look like a real model. CONSTITUTION: An acquired lymphedema animal model uses an animal without subcutaneous and lymphatic vessel by an iron and lymphatic vessel in the right leg and the right inguinal region. The animal is mammal and especially a mouth. A manufacturing method of the same comprises the following steps. The superficial inguinal node, deep inguinal node and popliteal node are cut. The right leg lymphatic vessel is blocked. To use the iron subcutaneous and lymphatic vessel are removed.
Abstract translation: 目的：提供获得性淋巴水肿动物模型及其生产方法，以形成比淋巴水肿动物更大的水肿，并且看起来像一个真实的模型。 构成：获得性淋巴水肿动物模型通过右腿和右腹股沟区域的铁和淋巴管，使用没有皮下和淋巴管的动物。 动物是哺乳动物，特别是嘴。 其制造方法包括以下步骤。 切开腹浅腹股沟，深腹股沟和al节。 右腿淋巴管堵塞。 使用铁皮下和淋巴管取出。
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公开(公告)号：KR1020130049670A
公开(公告)日：2013-05-14
申请号：KR1020110114820
申请日：2011-11-04
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q1/04 , C12Q2527/101 , C12Q2563/107
Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid, and a use thereof are provided to quickly diagnose using high concentration SYBR Green I with naked eye under natural light. CONSTITUTION: A primer set for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid contains sequence in sequence numbers 1-4. The Chrysanthemum chlorotic mottle viroid is SSHA6 strains or msim34 strains. A primer composition for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid contains the primer set. The composition further contains DNA polymerase, dNTPs, and a reaction buffer.
Abstract translation: 目的：用于环路介导的等温扩增用于检测菊花褪绿斑马疹的底漆组合物，并提供其用途，在自然光下用肉眼快速诊断高浓度SYBR Green I。 构成：用于检测菊花褪绿斑马疹的环介导等温扩增的引物组包含序列号1-4中的序列。 菊花褪绿斑马疹是SSHA6菌株或msim34菌株。 用于检测菊花褪绿斑驳病毒的环介导等温扩增的底漆组合物含有引物组。 该组合物还含有DNA聚合酶，dNTP和反应缓冲液。
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Patent Agency Ranking
公开(公告)号：KR1020120043924A
公开(公告)日：2012-05-07
申请号：KR1020100105222
申请日：2010-10-27
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/701 , C12Q1/6844
Abstract: PURPOSE: A primer set for detecting begomovirus is provided to accurately and quickly diagnose begomovirus infection from plant samples. CONSTITUTION: A primer set for detecting begomovirus comprises one or more kinds of primer sets selected among a primer set of: sequence numbers 1 and 2; sequence numbers 3 and 4; sequence numbers 5 and 6; sequence number 7 and 8; sequence numbers 9 and 10; sequence number 11 and 12; sequence numbers 13 and 14; sequence number 15 and 16; sequence numbers 17 and 18; and sequence numbers 19 and 20. The begomovirus includes tomato leaf curl virus, tomato yellow leaf curl virus, tobacco leaf curl virus, honeysuckle yellow vein virus, or sweet potato leaf curl virus.
Abstract translation: 目的：提供用于检测青霉素病毒的引物组，以准确快速地诊断植物样品中的胚胎病毒感染。 构成：用于检测霉菌病毒的引物组包括选自序列号1和2的引物组中的一种或多种引物组; 序列号3和4; 序列号5和6; 序列号7和8; 序列号9和10; 序列号11和12; 序列号13和14; 序列号15和16; 序列号17和18; 序列号19和20。枯叶病毒包括番茄叶卷曲病毒，番茄黄叶卷曲病毒，烟叶卷曲病毒，金银花黄色静脉病毒或甘薯叶卷曲病毒。
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公开(公告)号：KR1020090112597A
公开(公告)日：2009-10-28
申请号：KR1020090035785
申请日：2009-04-24
Applicant: 성균관대학교산학협력단 , 대한민국(농촌진흥청장)
Abstract: PURPOSE: A gene which is derived from sweet potato leaf curl infection DNA virus with an activity of suppressing gene silence after transcription is provided to obtain AC2 gene with sequence of the sequence number 16. CONSTITUTION: A gene derived from a sweet potato leaf curl infection DNA virus with an activity of suppressing gene silence after transcription is AC2 gene having sequence of the sequence number 16. An AC2 protein derived from a sweet potato leaf curl infection DNA virus is denoted by the sequence of the sequence number 17.
Abstract translation: 目的：提供具有抑制转录后基因沉默活性的甘薯叶卷曲感染DNA病毒的基因，以获得序列号为16的序列的AC2基因。构成：源自甘薯叶卷曲感染的基因 具有抑制转录后基因沉默的活性的DNA病毒是具有序列号16的序列的AC2基因。来自甘薯叶卷曲感染DNA病毒的AC2蛋白由序列号17的序列表示。
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公开(公告)号：KR102256363B1
公开(公告)日：2021-05-27
申请号：KR1020190049719
申请日：2019-04-29
Applicant: 성균관대학교산학협력단
Abstract: 본발명은토마토잎말림뉴델리바이러스 DNA-A 및 DNA-B를접종할수 있는감염성클론및 이의용도에관한것이다. 본발명의토마토잎말림뉴델리바이러스 DNA-A 및 DNA-B(ToLCNDV DNA-A 및 DNA-B)의염기서열을포함하는재조합플라스미드및 이를포함하는대장균및 아그로박테리움튜머페이션스를이용시, 매개충(insect vector) 없이도효과적으로작물에토마토잎말림뉴델리바이러스 DNA-A 및 DNA-B의감염을유발시킬수 있다. 따라서, 본발명을이용하여토마토잎말림뉴델리바이러스 DNA-A 및 DNA-B의기주범위및 기주와의상관관계등 다양한연구에응용할수 있으며, 이를통해바이러스감염으로인한경제적손실을사전에방지할수 있어, 관련분야에유용하게이용할수 있다.
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公开(公告)号：KR102093125B1
公开(公告)日：2020-03-25
申请号：KR1020180104618
申请日：2018-09-03
Applicant: 성균관대학교산학협력단 , 대한민국(농촌진흥청장)
IPC: G01N33/569
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