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    35.
    发明专利
    未知

    公开(公告)号：DK488682A

    公开(公告)日：1983-05-05

    申请号：DK488682

    申请日：1982-11-03

    Applicant: MILES LAB

    Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

    IPC: G01N33/78 ,  G01N

    Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or piasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

    CHEMICALLY EXCITABLE FLUORESCER-LABELED SPECIFIC BINDING ASSAY
    36.
    发明专利
    CHEMICALLY EXCITABLE FLUORESCER-LABELED SPECIFIC BINDING ASSAY 未知

    公开(公告)号：CA1133392A

    公开(公告)日：1982-10-12

    申请号：CA341540

    申请日：1979-12-10

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J

    IPC: G01N21/76 ,  G01N33/533 ,  G01N33/536 ,  G01N33/543 ,  G01N33/52

    Abstract: A specific binding assay method and reagent means for determining a ligand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium which employs a fluorescent substance, i.e. a fluorescer, as a label. The improvement comprises measuring the fluorescer-label by chemically exciting the label and measuring the resulting light emitted thereby. Chemical excitation of the label is preferably accomplished by exposure to a substance, such as a high energy intermediate produced by the reaction between hydrogen peroxide and highly reactive materials such as oxalyl chloride, oxamides and bis-oxalate esters. The assay may follow conventional homogeneous and heterogeneous formats. The improved assay is more convenient than conventional fluorescent binding assays by obviating the need for photogenic excitation of the fluorescent label.

    SPECIFIC BINDING ASSAY WITH AN ENZYME MODULATOR AS A LABELING SUBSTANCE
    37.
    发明专利
    SPECIFIC BINDING ASSAY WITH AN ENZYME MODULATOR AS A LABELING SUBSTANCE 未知

    公开(公告)号：CA1079632A

    公开(公告)日：1980-06-17

    申请号：CA288598

    申请日：1977-10-13

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  CHRISTNER JAMES E

    IPC: G01N33/536 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/542 ,  G01N33/543 ,  G01N33/16 ,  G01N31/14

    Abstract: A specific binding assay method employing, as a labeling substance, a reversibly binding enzyme modulator for the detection of a ligand in a liquid medium. The method follows conventional specific binding assay techniques of either the homogeneous or heterogeneous type wherein the liquid medium to be assayed is combined with reagent means that includes a labeled conjugate to form a binding reaction system having a bound-species and a free-species of the conjugate. The amount of conjugate resulting in the bound-species or the free-species is a function of the amount of ligand present in the liquid medium assayed. In the present invention, the labeled conjugate comprises a reversibly binding enzyme modulator covalently linked to a binding component of the binding reaction system. The distribution of the conjugate between the bound-species and the free-species is determined by addition of an enzyme whose modulator and measuring the resulting activity of the enzyme. The enzyme modulator may be a conventional enzyme inhibitor, preferably of the competitive type, or an allosteric effector.

    HYBRIDIZATION ASSAY EMPLOYING LABELED PAIRS OF HYBRID BINDING REAGENTS
    40.
    发明专利
    HYBRIDIZATION ASSAY EMPLOYING LABELED PAIRS OF HYBRID BINDING REAGENTS 未知

    公开(公告)号：CA1239580A

    公开(公告)日：1988-07-26

    申请号：CA469906

    申请日：1984-12-12

    Applicant: MILES LAB

    Inventor： CARRICO ROBERT J

    IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/52 ,  G01N33/53

    Abstract: A nucleic acid hybridization assay wherein the hybrid formed with the probe has binding sites for two specific binding reagents, one of which comprises a first label and the other a second label, interaction of the first and second labels providing a detectable response which is measurably different when the two labeled reagents are both bound to the same hybrid compared to when the two labeled reagents are not so bound. The formation of the hybrid assay product brings the two labels within a proximate interaction distance of one another, e.g., as in the cases of sequential catalyst (enzyme) interaction and energy transfer. Since the labels provide a response which is distinguishable when they are associated with a hybridized probe, no separation step is required.

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