公开(公告)号：CA2746632A1

公开(公告)日：2010-06-17

申请号：CA2746632

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2655018A1

公开(公告)日：2007-12-21

申请号：CA2655018

申请日：2007-06-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： SHEN GENE ,  PELUSO PAUL ,  LYLE JOHN

IPC: C12Q1/68 ,  C12P19/34

Abstract: Controlled initiation of primer extension in determination of nucleic aci d sequence information by incorporation of nucleotides or nucleotide analogs . Preferred aspects include photo-initiated extension through the use of pho to-cleavable blocking groups on termini of primer sequences followed by non- terminating primer extension using nucleotides or nucleotide analogs that ar e not extension terminators.

公开(公告)号：EP2029780A4

公开(公告)日：2010-03-31

申请号：EP07798630

申请日：2007-06-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： LYLE JOHN ,  PELUSO PAUL ,  SHEN GENE

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q2561/113 ,  C12Q2525/186 ,  C12Q2523/313

公开(公告)号：EP2370598A4

公开(公告)日：2012-06-13

申请号：EP09832251

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q2561/113 ,  C12Q2537/164 ,  C12Q2527/113 ,  C12Q2521/101 ,  C12Q2525/307 ,  C12Q2525/117

公开(公告)号：EP2245444A4

公开(公告)日：2014-04-16

申请号：EP09700236

申请日：2009-01-09

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： LUONG KHAI ,  LUNDQUIST PAUL ,  DALAL RAVI ,  LYLE JOHN ,  TURNER STEPHEN

IPC: G01N21/64 ,  G01N21/00

CPC classification number: C12M1/34 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/7786 ,  Y10T436/2525

公开(公告)号：EP1963536A4

公开(公告)日：2010-03-03

申请号：EP06848948

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HANZEL DAVID K ,  OTTO GEOFF ,  MURPHY DEVON ,  PELUSO PAUL ,  PHAM THANG ,  RANK DAVID R ,  MITSIS PAUL ,  CHRISTIANS FRED C ,  BIBILLO AREK ,  PARK INSIL ,  CLARK SONYA ,  LYLE JOHN

IPC: C12Q1/68

CPC classification number: C12N9/1252 ,  C07K2319/20 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Y207/07007 ,  Y02P20/52

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

公开(公告)号：EP3030683A4

公开(公告)日：2017-05-03

申请号：EP14834734

申请日：2014-08-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： SEBO LUBOMIR ,  HANES JEREMIAH ,  SHEN GENE ,  BROGLEY LOUIS ,  YUE STEPHEN ,  ZHENG FRANK ,  LAPIN YURI ,  LYLE JOHN ,  OSUNA HONEY ,  FEDOROV ANDREI

IPC: C12Q1/68 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/10 ,  G01N21/64 ,  G01N23/223

CPC classification number: C07H19/10 ,  C07F9/65586 ,  C07F9/65616

Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Abstract translation: 提供受保护的荧光试剂化合物及其合成方法。 这些化合物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，可以在理想的波长下以高灵敏度检测化合物，允许化合物特异性地被目标生物分子识别的结合元件和保护性屏蔽元​​件，其减少荧光染料元素之间的不希望的接触 和结合的目标生物分子，并因此减少了荧光染料元件对结合的目标生物分子的光损伤。

公开(公告)号：EP2002019A4

公开(公告)日：2009-07-15

申请号：EP07754529

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF

IPC: C12N11/00 ,  C40B40/04

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00427 ,  B01J2219/0045 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y20/00 ,  B82Y30/00 ,  C12Q1/6837 ,  Y10T29/49002 ,  C12Q2565/629 ,  C12Q2527/127

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.