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公开(公告)号：CA2746632C
公开(公告)日：2020-06-30
申请号：CA2746632
申请日：2009-12-10
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH , PUGLISI JOSEPH
IPC: C12Q1/68 , C12Q1/6809 , C12Q1/6869
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
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公开(公告)号：AU2009297145A1
公开(公告)日：2010-04-01
申请号：AU2009297145
申请日：2009-09-16
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： MAXHAM KENNETH MARK , SORENSON JON , EID JOHN , MARKS PATRICK , TRAVERS KEVIN , GRAY DONALD , EMIG ROBIN , CHAISSON MARK , FLUSBERG BENJAMIN
IPC: G01N21/00
Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.
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公开(公告)号：WO2010144151A3
公开(公告)日：2011-05-12
申请号：PCT/US2010001692
申请日：2010-06-11
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , TURNER STEPHEN , KORLACH JONAS , SCHADT ERIC
Inventor： FLUSBERG BENJAMIN , TURNER STEPHEN , KORLACH JONAS , SCHADT ERIC
IPC: C12Q1/68
CPC classification number: G01N33/542 , G01N33/6818 , Y10T436/13 , Y10T436/143333
Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of analytical reactions in which protein synthesis is occurring. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance, inhibit, or otherwise affect such reactions including, but not limited to, affecting the reaction rate, processivity, fidelity, duration, and the like.
Abstract translation: 本发明一般涉及用于进行蛋白质合成发生的分析反应的单分子实时分析的组合物，方法和系统。 分析这些反应的能力提供了研究这些反应的机会，并且潜在地鉴定影响这些反应的因素和/或方法，例如增强，抑制或以其他方式影响这些反应，包括但不限于影响 反应速度，持续性，持续时间等。
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公开(公告)号：WO2010068289A3
公开(公告)日：2010-10-14
申请号：PCT/US2009006517
申请日：2009-12-10
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
Inventor： FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：CA2720247C
公开(公告)日：2020-07-14
申请号：CA2720247
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： EID JOHN , TURNER STEPHEN , DALAL RAVI , FLUSBERG BENJAMIN , KORLACH JONAS , LIN STEVEN , FEHR ADRIAN , CHRISTIANS FRED , EMIG ROBIN
Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.
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Patent Agency Ranking
公开(公告)号：AU2009251881B2
公开(公告)日：2015-03-26
申请号：AU2009251881
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： TURNER STEPHEN , CHRISTIANS FRED , FEHR ADRIAN , FLUSBERG BENJAMIN , EMIG ROBIN , DALAL RAVI , KORLACH JONAS , LIN STEVEN , EID JOHN
Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.
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公开(公告)号：AU2008331824B2
公开(公告)日：2014-07-24
申请号：AU2008331824
申请日：2008-12-03
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： FLUSBERG BENJAMIN , EID JOHN , OTTO GEOFF , KORLACH JONAS , ROITMAN DANIEL , HARDENBOL PAUL
IPC: G01N33/53 , G01N33/535 , G01N33/573
Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.
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公开(公告)号：CA2707600C
公开(公告)日：2018-02-20
申请号：CA2707600
申请日：2008-12-03
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： KORLACH JONAS , ROITMAN DANIEL , EID JOHN , OTTO GEOFF , HARDENBOL PAUL , FLUSBERG BENJAMIN
IPC: C12Q1/00 , C12M1/34 , C12M1/40 , G01N33/53 , G01N33/573
Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.
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公开(公告)号：AU2011326026A1
公开(公告)日：2013-05-02
申请号：AU2011326026
申请日：2011-11-11
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
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公开(公告)号：CA2738648A1
公开(公告)日：2010-04-01
申请号：CA2738648
申请日：2009-09-16
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： MAXHAM KENNETH MARK , SORENSON JON , EID JOHN , MARKS PATRICK , TRAVERS KEVIN , GRAY DONALD , EMIG ROBIN , CHAISSON MARK , FLUSBERG BENJAMIN
IPC: G01N21/00
Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.
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