公开(公告)号：WO2022104224A1

公开(公告)日：2022-05-19

申请号：PCT/US2021/059410

申请日：2021-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PODHASKY, Joseph ,  KANE, Nathan ,  REED, Mark

IPC: G01N1/34 ,  C12N15/10 ,  G01N1/40 ,  G01N33/543 ,  G01N35/00

Abstract: A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.

公开(公告)号：WO2022006368A2

公开(公告)日：2022-01-06

申请号：PCT/US2021/040037

申请日：2021-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KORE, Anilkumar ,  ANNAMALAI, Senthilvelan ,  MUTHIAN, Shanmugasundaram ,  POTTER, Robert ,  VONDERFECHT, Tyson

IPC: C07H21/00 ,  C07H1/00 ,  C12N15/00 ,  A61K31/7088 ,  A61K38/45 ,  A61K47/549 ,  C12N15/11 ,  C12N2310/3231 ,  C12N2310/3519 ,  C12N2320/32 ,  C12N9/1247 ,  C12Y207/07006

Abstract: This specification generally relates to trinucleotide RNA cap analogs, methods of use thereof, and kits comprising same. In particular, the trinucleotide cap analogs provided herein permit ready detection and/or isolation of capped RNA transcripts in vitro and translation of capped mRNAs in vivo.

公开(公告)号：WO2021236466A1

公开(公告)日：2021-11-25

申请号：PCT/US2021/032614

申请日：2021-05-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： FOX, Daniel N. ,  FOX, Nathan M. ,  HARRIS, Rodney C.

IPC: G01N15/14

Abstract: Disclosed is a system for use in a flow cytometer for unclogging obstructions in a nozzle from salt crystal formations and clumps of cells or debris. A nozzle system is moved to a docking station so that a nozzle tip is seated in the docking station. Deionized water is pushed through the nozzle in a reverse direction to a waste port so that the nozzle is flushed. The nozzle system can remain in the docked position during nonuse so that salt crystals do not form in or on the nozzle.

公开(公告)号：WO2021178809A1

公开(公告)日：2021-09-10

申请号：PCT/US2021/021104

申请日：2021-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： POTTER, Robert ,  NETUSCHIL, Nikolai

IPC: C12Q1/686 ,  C12N15/10 ,  C40B50/00 ,  C12N15/1031 ,  C40B50/06

Abstract: The present disclosure generally relates to compositions and methods for the synthesis of nucleic acid molecules with low error rates. Provided, as examples, are compositions and methods for high throughput synthesis and assembly of nucleic acid molecules, in many instances, with high sequence fidelity. In many instances, thermostable mismatch recognition proteins (e.g., thermostable mismatch binding protein, thermostable mismatch endonucleases) will be present in compositions and use methods provided.

公开(公告)号：WO2021158555A1

公开(公告)日：2021-08-12

申请号：PCT/US2021/016224

申请日：2021-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： JONES, Nephi D. ,  BROWN, Jason D.

IPC: B01F7/00 ,  B01F15/00 ,  B01F15/06

Abstract: A mixing system for mixing a liquid includes a first impeller segment (170A) having a first mount (172A) and a first mixing blade (174A) secured to the first mount (172A) and a second impeller segment (170B) having a second mount (172B) and a first mixing blade (174B) secured to the second mount (172B), the second impeller segment (170B) being separate and discrete from the first impeller segment (170A). One or more drive members are secured to the first impeller segment (170A) and the second impeller segment (170B) for concurrently rotating the first impeller segment (170A) and the second impeller segment (170B) about a rotational axis (171). The first impeller segment (170A) and the second impeller segment (170B) are secured to the one or more drive members so that a plane (210) extending normal to the axis (171) of rotation intersects with the first mixing blade (174A) of the first impeller segment (170A) and the first mixing blade (174A) of the second impeller segment (170B).

公开(公告)号：WO2021104855A1

公开(公告)日：2021-06-03

申请号：PCT/EP2020/081657

申请日：2020-11-10

Applicant: THERMO FISHER SCIENTIFIC (BREMEN) GMBH ,  LIFE TECHNOLOGIES CORPORATION ,  FEI DEUTSCHLAND GMBH

Inventor： MAKAROV, Alexander ,  WARD, Michael ,  DAUM, Rainer

IPC: G01N21/64 ,  G01N21/71 ,  G01N35/00 ,  C12Q1/686 ,  C12Q1/6872 ,  H01J49/04 ,  H01J49/16

Abstract: Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device (110) includes an optical assembly (112), a sample stage (114), and a receiver (116). The optical assembly (112) is disposed in an inverted position below the sample stage (114) and the receiver (116) is positioned above the sample stage (112). The optical assembly enables imaging of a sample disposed on the sample stage (114). The optical assembly (112) also enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume (20) toward the receiver (116).

公开(公告)号：WO2021092523A1

公开(公告)日：2021-05-14

申请号：PCT/US2020/059581

申请日：2020-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LEONG, Harrison ,  BAUDO, Charles ,  HIGDON, Charles, Wendell

IPC: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6827

Abstract: Systems and methods for detecting microsatellite instability in a biological sample are described. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is measured from fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample via polymerase chain reaction (PCR). The nucleic acid sequences correspond to a plurality of different microsatellite loci and are obtained using a plurality of PCR primers configured to flank a plurality of microsatellite loci of a biological sample. When the PCR primers and the biological sample are combined and subjected to PCR amplification, fluorescently labeled DNA fragments are generated comprising the plurality of microsatellite loci. Fluorescent data obtained from the plurality of fluorescently labelled microsatellite loci are used to classify microsatellite instability of the biological sample.

公开(公告)号：WO2021092299A1

公开(公告)日：2021-05-14

申请号：PCT/US2020/059295

申请日：2020-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： HIGDON, Charles ,  LEONG, Harrison ,  BAUDO, Charles ,  BORNARTH, Carole ,  SCHREIBER, Edgar

IPC: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6827

Abstract: Systems, primers, kits, and methods for detecting microsatellite instability in a biological sample are described. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is measured from fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample via polymerase chain reaction (PCR). The nucleic acid sequences correspond to a plurality of different microsatellite loci and are obtained using a plurality of PCR primers configured to flank a plurality of microsatellite loci of a biological sample. When the PCR primers and the biological sample are combined and subjected to PCR amplification, fluorescently labeled DNA fragments are generated comprising the plurality of microsatellite loci. Fluorescent data obtained from the plurality of fluorescently labelled microsatellite loci are used to classify microsatellite instability of the biological sample.

公开(公告)号：WO2021081381A1

公开(公告)日：2021-04-29

申请号：PCT/US2020/057138

申请日：2020-10-23

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LTD

Inventor： YEO, Steven Huei ,  HON, Siu Wee ,  WEI, Han ,  PECK, Yvonne ,  LEE, Way Xuang ,  SHI, Benyong ,  CHAN, Chee Wai ,  LIM, Beng Heng ,  SHANMUGAM, Dhanaraj ,  TEO, Stacey ,  THAM, Jun Yan ,  CHONG, Kok Shyong ,  LIM, Li Yang ,  GORDON, Michael ,  ANDRONIKOU, Nektaria ,  ROSSMAN, Chris ,  DE MOLLERAT DU JEU, Xavier ,  FORKE, Ulrich

IPC: C12M1/42

Abstract: The present disclosure provides electroporation cartridges for single use electroporation as well as electroporation cartridges for automated batch processing, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber defined by an elongate body, a first electrode at a proximal end and a second electrode at a distal end of a chamber. Electroporation systems of the disclosure include one or more components including a pulse generator, compartments for placing either flow-through or single use electroporation cartridges, components for storage of cells, cooling and pre-cooling mechanisms, removably insertable modular casings having compartments for holding and arranging electroporation system and reagent components, one or more pumps for moving sample through the system, and processors and controllers.

公开(公告)号：WO2021055579A1

公开(公告)日：2021-03-25

申请号：PCT/US2020/051236

申请日：2020-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PHELPS, Mwita

IPC: C12N1/00 ,  C12N5/00 ,  C12N5/07 ,  C12N5/071 ,  C12Q1/04

Abstract: Described herein are efficiently dissolving tablets of dry cell culture media, feeds, supplements, media subgroups, buffer concentrates, or media components useful in culturing cells or microorganisms, methods of manufacturing, and methods of use. In particular, formulations of tableted cell culture media feeds, supplements, media subgroups, or buffer concentrates are described.