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公开(公告)号：KR1020150039321A
公开(公告)日：2015-04-10
申请号：KR1020130117750
申请日：2013-10-02
Applicant: 한국식품연구원
CPC classification number: A23L33/00 , A23L19/03 , A23V2002/00 , A23V2300/46
Abstract: 수확직후의신선한과실류, 채소류를 10-30℃에서 5-20분이내의시간동안 30-70 MPa 범위의조건에서압력처리하여페놀류생합성유전자를인위적으로발현시켜건강기능성분을향상시키는방법이개시된다.
Abstract translation: 在本发明中，公开了一种富含健康功能成分的方法，其在30〜70MPa压力下在10〜30摄氏度收获5〜20分钟后立即对新鲜水果和蔬菜进行处理，从而表现出苯酚生物合成基因 以人为的方式，从而丰富健康功能组分。
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公开(公告)号：KR101439158B1
公开(公告)日：2014-09-18
申请号：KR1020120077731
申请日：2012-07-17
Applicant: 한국식품연구원 , 대한민국 (식품의약품안전처장)
IPC: C12N15/115 , C12Q1/68 , C12Q1/04
CPC classification number: Y02A50/451
Abstract: 본 발명은 식중독균 검출 키트 및 이를 이용하여 3종의 식중독균을 동시에 검출하는 방법에 관한 것이다. 일 구체예에 따른 식중독균 검출용 키트를 사용하면, 3종의 식중독균을 동시에 효율적으로 검출할 수 있다.
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公开(公告)号：KR101374496B1
公开(公告)日：2014-03-26
申请号：KR1020120126267
申请日：2012-11-08
Applicant: 한국식품연구원 , (주)선해에프앤에스
IPC: A23B4/03 , A23B4/18 , A23B4/20 , A23L3/015 , A23L3/3472
CPC classification number: A23B4/03 , A23B4/20 , A23L3/015 , A23L3/3472
Abstract: The present invention relates to a freshness maintaining method of fresh fish using a high pressure processing system and processed fresh fish produced by the method, and more specifically, to: a method of reducing bacteria from the fresh fish, extending the storage period of the fresh fish, and maintaining the texture of the fresh fish by processing a raw material at 10-25°C, in 25-300 MPa, for 2-40 minutes for 1-2 times using high pressure; and the processed fresh fish produced by the method. [Reference numerals] (AA) Raw material; (BB) Washing; (CC) Cutting treatment for twice to 3 times; (DD) Individual immersion in a film filled with 1-1.5 L of immersion water; (EE) Sealing; (FF) Primary high-pressure processing (100 MPa, 20 minutes, 25째C); (GG) Dehydration for 30 minutes; (HH) Individual vacuum packaging; (II) Second high-pressure processing (100 MPa, 20 minutes, 25째C); (JJ) Sample; (KK) Natural antimicrobial agent, Bamboo salt
Abstract translation: 本发明涉及使用高压处理系统的新鲜鱼的保鲜方法和通过该方法生产的加工鲜鱼，更具体地涉及：从鲜鱼中减少细菌的方法，延长新鲜鱼的保存期 鱼，并通过在25〜300MPa下加工10-25℃的原料，高压保持1-2-40分钟维持新鲜鱼的质地; 和通过该方法生产的经加工的新鲜鱼。 （附图标记）（AA）原料; （BB）洗涤; （CC）切割治疗2〜3次; （DD）个人浸入装有1-1.5L浸水的薄膜; （EE）密封; （FF）初级高压处理（100MPa，20分钟，25℃）; （GG）脱水30分钟; （HH）个人真空包装; （II）第二次高压加工（100MPa，20分钟，25℃）; （JJ）样品; （KK）天然抗菌剂，竹盐
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公开(公告)号：KR1020140011086A
公开(公告)日：2014-01-28
申请号：KR1020120077731
申请日：2012-07-17
Applicant: 한국식품연구원 , 대한민국 (식품의약품안전처장)
IPC: C12N15/115 , C12Q1/68 , C12Q1/04
CPC classification number: Y02A50/451 , C12N15/115 , C12N2310/16 , C12Q1/689 , C12Q2525/205 , C12Q2537/143 , C12R1/19 , C12R1/42 , C12R1/445
Abstract: The present invention relates to a food poisoning bacteria detection kit and a method for detecting the three kinds of food poisoning bacteria by using the same. In accordance with an embodiment of the present invention, the food poisoning bacteria detection kit can effectively detect the three kinds of the food poisoning bacteria at the same time. The food poisoning bacteria detection kit includes RNA aptamer including base sequence of sequence number 1 specifically combined with ompC protein of Salmonella; RNA aptamer including the base sequence of sequence number 2 specifically combined with teichoic acid of Staphylococcus; and RNA aptamer including the base sequence of sequence number 3 specifically combined with lipopolysaccharide (LPS) of Escherichia coli O157:H7.
Abstract translation: 本发明涉及一种食物中毒细菌检测试剂盒和使用该食物中毒细菌的三种食物中毒细菌的检测方法。 根据本发明的实施例，食物中毒细菌检测试剂盒可以同时有效地检测三种食物中毒细菌。 食物中毒细菌检测试剂盒包括RNA适体，其包括序列号1的碱基序列特异性结合沙门氏菌的ompC蛋白; RNA适体包括序列号2的碱基序列与葡萄球菌的磷壁酸特异性结合; 和包含特异性结合大肠杆菌O157：H7的脂多糖（LPS）的序列号3的碱基序列的RNA适体。
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公开(公告)号：KR101292636B1
公开(公告)日：2013-08-02
申请号：KR1020110077756
申请日：2011-08-04
Applicant: 한국식품연구원
IPC: C12N15/115 , C12Q1/68 , C12Q1/04
CPC classification number: C12Q1/689 , C12N15/111 , C12N2310/16 , C12N2320/10 , C12Q1/04 , G01N33/5017 , G01N33/54366 , G01N33/553 , G01N33/56911 , G01N33/56916 , G01N33/56938
Abstract: 본 발명은 식중독균 검출 센서용 슬라이드 칩 및 그 제조방법에 관한 것으로서, 보다 상세하게는 금속이 코팅된 기판; 상기 금속에 결합될 수 있는 치환기가 디옥시티미딘(deoxythymidine, dT)의 5'말단에 위치한 링커; 및 3'말단에 위치한 폴리 아데닐산 꼬리(poly A tail)에 의해 상기 링커와 결합되는 식중독균 유래 RNA 앱타머를 포함함으로써 신속하고 정확하게 식품 병원균을 검출할 수 있는 식중독균 검출 센서용 슬라이드 칩 및 그 제조방법에 관한 것이다.
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Patent Agency Ranking
公开(公告)号：KR1020130063012A
公开(公告)日：2013-06-13
申请号：KR1020137007651
申请日：2011-12-14
Applicant: 한국식품연구원
IPC: C12N15/115 , C12Q1/68 , A61K31/713
CPC classification number: A61K31/7115 , C12N15/115 , C12N2310/16 , C12Q1/689 , C12Q2600/156 , Y02A50/473 , A61K31/713
Abstract: PURPOSE: An RNA aptamer is provided to detect E.coli O157:H7 by specifically binding to E.coli O157:H7, to prevent E.coli O157:H7, and to prevent food spoilage. CONSTITUTION: An RNA aptamer has a base sequence selected from a group consisting of sequence numbers 1-4, which specifically binds to E.coli O157:H7. Urecil(U) and cytosine(C) of sequence number 1 or 2 have 2' hydroxyl group which is substituted with a fluorine group. A biosensor for detecting E.coli O157:H7 contains the RNA aptamer. A pharmaceutical composition for preventing or treating diseases selected from a group consisting of food poisoning, hemolytic uremic syndrome(HUS), and hemolytic anemia contains the RNA aptamer. A food additive composition contains the RNA aptamer.
Abstract translation: 目的：提供RNA适体以通过特异性结合大肠杆菌O157：H7来检测大肠杆菌O157：H7，以防止大肠杆菌O157：H7，并防止食物变质。 构成：RNA适体具有选自序列号1-4的碱基序列，其特异性结合大肠杆菌O157：H7。 序列号1或2的Urecil（U）和胞嘧啶（C）具有被氟基团取代的2'羟基。 用于检测大肠杆菌O157：H7的生物传感器含有RNA适体。 用于预防或治疗选自食物中毒，溶血性尿毒综合征（HUS）和溶血性贫血的疾病的药物组合物含有RNA适体。 食品添加剂组合物含有RNA适体。
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公开(公告)号：KR1020130028564A
公开(公告)日：2013-03-19
申请号：KR1020110092204
申请日：2011-09-09
IPC: A61K36/258 , A61P35/00 , A61P9/00
Abstract: PURPOSE: A method for extracting useful ingredients of ginseng by high pressure and enzyme decomposition is provided to cheaply extract useful ingredient. CONSTITUTION: A method for extracting useful ingredients of ginseng by high pressure and enzyme decomposition comprises: a step of adding 20-30 wt% of raw ginseng to water and adding 0.5-1.2 wt% of an enzyme based on a substrate in a high pressure reactor; and a step of controlling the high pressure reactor at 100-200 MPa and at 55-60 deg. C for 24-48 hours. The enzyme contains 0.1-3 wt% of malt, 0.1-3 wt% of thermamyl, 0.1-3 wt% of viscozyme, and 0.1-3 wt% of cellulast. [Reference numerals] (AA) Comparison between a current red ginseng manufacturing process and a high pressure enzyme decomposing process; (BB) Raw ginseng; (CC) 55% ethanol; (DD) Steaming and drying; (EE) Extracting(70°C); (FF,II) Filtering; (GG) Concentrating; (HH) Water; (JJ) Mixing; (KK) Aging and sterilizing; (LL) Bottling; (MM) Packaging; (NN) Red ginseng extract; (OO) Manufacturing process; (PP) Advantage and disadvantage; (QQ) Energy cost(based on 1ton of raw ginseng); (RR) Current producing process; (SS) High pressure decomposition; (T1) Steaming: 92-95°C; (T2) Drying: 60°C, 15days; (T3) Extracting: 2-3hours(70°C); (UU) 50% of manufacturing cost; (V1) Steaming-195,000 won; (V2) Drying-1,040,000 won; (V3) Extracting/concentrating-3,087,000 won; (V4) Total-4,322,000 won; (WW) High pressure decomposition(55°C,24hours); (XX) Low cost; (YY) High pressure enzyme decomposition 415,000 won
Abstract translation: 目的：提取人参高压和酶分解有用成分的方法，以廉价提取有用成分。 构成：通过高压和酶分解提取人参有用成分的方法包括：将20-30重量％的人参加入水中，并在高压下加入0.5-1.2重量％的基于底物的酶的步骤 反应堆; 以及在100-200MPa和55-60℃控制高压反应器的步骤。 C为24-48小时。 该酶含有0.1-3重量％的麦芽，0.1-3重量％的高酰基，0.1-3重量％的粘合酶和0.1-3重量％的蜂窝状乳液。 （附图标记）（AA）当前红参制造工序与高压酶分解工序的比较; （BB）人参; （CC）55％乙醇; （DD）蒸，干; （EE）萃取（70℃）; （FF，II）过滤; （GG）浓缩; （HH）水; （JJ）混合; （KK）老化消毒; （LL）装瓶; （MM）包装; （NN）红参提取物; （OO）制造工艺; （PP）优势和劣势; （QQ）能源成本（以人参1吨为单位）; （RR）当前生产过程; （SS）高压分解; （T1）蒸汽：92-95℃; （T2）干燥：60℃，15天; （T3）提取：2-3小时（70°C）; （UU）制造成本的50％; （V1）蒸 - 195,000韩元; （V2）干燥 - 104万韩元; （V3）提取/集中 - 3,087,000韩元; （V4）合计-43.22万韩元; （WW）高压分解（55℃，24小时）; （XX）成本低; （YY）高压酶分解415,000韩元
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公开(公告)号：KR101195254B1
公开(公告)日：2012-10-29
申请号：KR1020110080706
申请日：2011-08-12
Applicant: 한국식품연구원
IPC: G01N33/533 , G01N33/543 , G01N33/68
Abstract: 본 발명에 의하여, C-반응성 단백질을 슬라이드에 고정시켜 단백질 칩을 제조하는 단계, 분석하고자 하는 단백질에 특이적으로 결합하는 항체를 스트렙트아비딘과 혼합하여 형광나노입자로 표지시키는 단계, 상기 항체를 혼합경쟁적으로 면역반응시키는 단계, 형광카메라로 분석하는 단계에 있어서, 상기 C-반응성 단백질을 슬라이드에 고정화하기 위하여, 3-아미노프로필트리메톡시실란으로 개질된 슬라이드를 제조하는 단계, 3-아미노프로필트리메톡시실란으로 개질된 슬라이드를 수화하는 단계, 글루타르 알데히드 용액을 사용하여 상기 개질된 슬라이드를 활성화시키는 단계, 30-70mM 인산버퍼용액(pH 6.5-7.8)에 C-반응성 단백을 0.01-0.5㎎/㎖ 농도로 용해하여 고정화용 항원용액을 제조하는 단계, 스포팅 가이드 상에 상기 슬라이드를 포함하는 페트리디쉬를 올려놓고 상기 제조한 항원용액을 1-100㎕ 적하점에 스포팅하는 단계, 및 상기 단계에 의하여 준비된 슬라이드를 1-6시간 반응시켜 항원을 고정화하는 항원고정화 면역형광 슬라이드의 제조방법 및 이에 의하여 제조되는 면역형광 슬라이드가 개시된다.
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公开(公告)号：KR1020120095804A
公开(公告)日：2012-08-29
申请号：KR1020120016963
申请日：2012-02-20
Applicant: 한국식품연구원
CPC classification number: C12N9/6481 , A23L3/0155 , A23L5/17 , A23L27/00 , A23L27/21 , A23L29/06 , A61K38/48 , A61K38/51 , C12N9/48 , C12N9/485 , C12N9/52 , C12N9/6427 , C12Q1/37 , C12Y304/00
Abstract: PURPOSE: A method of using high pressure tolerance enzyme is provided to enhance reaction rate and/or reaction yield. CONSTITUTION: A method of using high pressure tolerance enzyme includes a step of precessing the enzyme at high pressure. the high pressure tolerance enzyme is α-chymotrypsin, pepsin, trypsin, trypsin acetylated, flavourzyme, protease E or alcalase. The high pressure is 100-400 MPa. The method is functional component enzymic synthesis method, decomposing protein, carbohydrate, or lipid method, bioactive substance sampling method, and deformation of protein ezyme method. The high pressure continues for 60-300 minutes. The method includes heat treatment at 40 deg. Celsius or greater for 2 minutes or greater. In the method, the high pressure processing and the thermal process are processed at the same time.
Abstract translation: 目的：提供使用高耐压酶的方法，以提高反应速率和/或反应产率。 构成：使用高压耐受酶的方法包括在高压下进行酶的步骤。 高压耐受酶是α-胰凝乳蛋白酶，胃蛋白酶，胰蛋白酶，胰蛋白酶乙酰化，风味酶，蛋白酶E或alcalase。 高压为100-400MPa。 该方法是功能组分酶合成方法，分解蛋白质，碳水化合物或脂质法，生物活性物质取样方法和蛋白酶法的变形。 高压持续60-300分钟。 该方法包括在40度的热处理。 摄氏或更高2分钟或更长时间。 在该方法中，高压处理和热处理同时被处理。
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