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    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE
    42.
    发明专利
    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE 未知

    公开(公告)号：CA2110050A1

    公开(公告)日：1992-12-10

    申请号：CA2110050

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： ADAMCZYK JANINA ,  BERRY DANIEL S ,  JOU YI-HER ,  STROUPE STEPHEN D

    IPC: G01N33/531 ,  G01N33/538 ,  G01N33/543 ,  G01N33/547 ,  G01N33/548

    Abstract: 2110050 9221772 PCTABS00017 The present invention includes novel assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyte on the solid phase even in the presence of polyanionic non-specific binding blockers.

    DEVICES FOR PERFORMING ION-CAPTURE BINDING ASSAYS
    43.
    发明专利
    DEVICES FOR PERFORMING ION-CAPTURE BINDING ASSAYS 未知

    公开(公告)号：CA2110049A1

    公开(公告)日：1992-12-10

    申请号：CA2110049

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： JOU YI-HER ,  STROUPE STEPHEN D

    IPC: G01N33/545 ,  G01N33/538 ,  G01N33/543 ,  G01N33/558 ,  G01N33/574

    Abstract: 2110049 9221980 PCTABS00018 This invention presents novel assay devices employing a capture reagent, involving a specific binding member attached to a charged substance, and a porous material containing a capture or reaction zone that is oppositely charged with respect to the capture reagent. In one embodiment, a test sample suspected of containing the analyte of interest is contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged capture or reaction zone to attract, attach, and immobilize the capture reagent/analyte complex. With an appropriate indicator reagent, both sandwich and competitive assays can be performed.

    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS
    46.
    发明专利
    LIPOSOME BASED HOMOGENEOUS IMMUNOASSAY FOR DIAGNOSTIC TESTS 未知

    公开(公告)号：AU2006988A

    公开(公告)日：1989-02-02

    申请号：AU2006988

    申请日：1988-07-27

    Applicant: ABBOTT LAB

    Inventor： JOU YI-HER ,  HU ROGER C ,  LAGOCKI PETER A

    IPC: G01N33/544 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  G01N33/76 ,  C07K15/12 ,  G01N33/53 ,  G01N33/563 ,  G01N33/92

    Abstract: The present invention provides for novel homogeneous immunoassay systems involving complement-mediated lysis of marker-encapsulating lipid vesicles (liposomes) for detection of analyte in a fluid sample. Antibody sensitized liposomes (the first reagent) are sequentially incubated with an analyte-containing sample, and optionally "dummy" liposomes, which do not contain encapsulated marker, a second antibody (the second reagent), and finally with a complement source such as plasma. Complement is activated by the liposome-antibody-antigen-second antibody complex causing liposome lysis and a concomitant release of marker. Antibody of the first reagent may be an anti-analyte F(ab min )2 antibody fragment, or an anti-analyte Fab min antibody fragment. Antibody of the second reagent may be provided in either soluble form, or in insoluble form e.g., bound onto carboxylated polystyrene particles or coupled to a third antibody in the form of a "double antibody" immune precipitate. Also provided are methods for preparing antibody sensitized liposomes in the presence of a polysaccharide capable of forming a reversible gel and methods for preparing derivatized Fab min antibody fragments for coupling to lipid vesicles.

    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE
    47.
    发明专利
    ION-CAPTURE ASSAYS USING A BINDING MEMBER CONJUGATED TO CARBOXYMETHYLAMYLOSE 未知

    公开(公告)号：CA2110050C

    公开(公告)日：2004-02-24

    申请号：CA2110050

    申请日：1992-04-10

    Applicant: ABBOTT LAB

    Inventor： ADAMCZYK JANINA ,  BERRY DANIEL S ,  JOU YI-HER ,  STROUPE STEPHEN DENHAM

    IPC: G01N33/531 ,  G01N33/538 ,  G01N33/543 ,  G01N33/547 ,  G01N33/548

    Abstract: The present invention includes novel assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyze of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to th e oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyze on the solid phase even in the presence of polyanionic non-specific binding blockers.

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