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公开(公告)号：KR1020110044343A
公开(公告)日：2011-04-29
申请号：KR1020090100960
申请日：2009-10-23
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/12 , G01N33/573
Abstract: PURPOSE: A method for distinguishing frozen-thawed meat and refrigerated meat is provided to ensure simple and easy use without expensive equipment. CONSTITUTION: A composition for distinguishing frozen-thawed meat and refrigerated meat contains an antibody with respect to aspartate aminotransferase(AST) allozyme. AST allozyme is mitochondria AST. The meat is beef, pork, or chicken. A method for distinguishing frozen-thawed meat and refrigerated meat comprises: a step of preparing meat juice as a biological sample; and a step of performing electrophoresis of the meat juice to detect AST allozyme.
Abstract translation: 目的：提供一种区分冷冻解冻肉和冷藏肉的方法，以确保简单易用，无需昂贵的设备。 构成：用于区分冷冻解冻的肉和冷冻肉的组合物含有针对天冬氨酸氨基转移酶（AST）同种异体酶的抗体。 AST等位酶是线粒体AST。 肉是牛肉，猪肉或鸡肉。 一种区分冷冻解冻肉和冷冻肉的方法包括：制备肉汁作为生物样品的步骤; 以及进行肉汁的电泳以检测AST同种异体酶的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020120051196A
公开(公告)日：2012-05-22
申请号：KR1020100112506
申请日：2010-11-12
Applicant: 주식회사 바이오노트 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A kit for diagnosing bovine tuberculosis containing monoclonal antibodies and polyclonal antibodies is provided to effectively measure tuberculosis attack. CONSTITUTION: A monoclonal antibody which specifically binds to an epitope is denoted by an amino acid sequence of sequence number 8 in a bovine interferon gamma receptor-binding region. The monoclonal antibody is produced from hydridoma cells(deposit number KCTC 11784BP). A kit for diagnosing bovine contains monoclonal antibody or antigen binding fragment and anti-bovine interferon gamma polyclonal antibody.
Abstract translation: 目的：提供用于诊断含有单克隆抗体和多克隆抗体的牛结核的试剂盒，以有效测量结核病的发作。 构成：特异性结合表位的单克隆抗体由牛干扰素γ受体结合区中的序列号8的氨基酸序列表示。 单克隆抗体由Hydridoma细胞（保藏号KCTC 11784BP）产生。 用于诊断牛的试剂盒含有单克隆抗体或抗原结合片段和抗牛干扰素γ多克隆抗体。
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公开(公告)号：KR101263913B1
公开(公告)日：2013-05-14
申请号：KR1020100112506
申请日：2010-11-12
Applicant: 주식회사 바이오노트 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 소의인터페론감마에특이적으로결합하는단일클론항체및 폴리클론항체를포함하는우결핵진단키트및 이를이용한우결핵진단방법에관한것이다. 일구체예에따른소의인터페론감마에특이적으로결합하는단일클론항체, 폴리클론항체를포함하는우결핵진단키트및 이를이용한우결핵진단방법에따르면, 소과동물의우결핵발병여부를효율적으로측정할수 있다.
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公开(公告)号：KR1020110052295A
公开(公告)日：2011-05-18
申请号：KR1020090109275
申请日：2009-11-12
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.
Abstract translation: 目的：提供使用皮内诊断组合物诊断牛结核病的方法，以容易地制备大量皮内诊断组合物并确保诊断准确性。 构成：用于测试牛结核的皮内诊断组合物含有Esat6，HspX，PhoS，MPB70，MPB64，MPB83，Ag85和SahH的重组蛋白。 使用含有重组蛋白质的皮内诊断组合物进行用于诊断除人以外的哺乳动物结核病的方法。 通过从牛分枝杆菌中分离并进行PCR来制备重组蛋白。
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5.发明申请
公开(公告)号：WO2010005273A2
公开(公告)日：2010-01-14
申请号：PCT/KR2009/003832
申请日：2009-07-13
Applicant: 서울대학교산학협력단 , 주식회사 바이오노트 , 박재학 , 석승혁 , 조영식 , 하건우 , 오진식 , 오연경
IPC: C12N15/11
CPC classification number: C07K14/79 , A01K67/0278 , A01K2217/052 , A01K2227/40 , A01K2267/01
Abstract: 본 발명은 인간 락토페린 유전자를 포함하는 재조합 발현 벡터로 형질전환되어 인간 락토페린을 대량생산하는 제브라피쉬, 상기 형질전환된 제브라피쉬로부터 인간 락토페린을 대량생산하는 방법, 및 상기 형질전환된 제브라피쉬를 유효성분으로 함유하는 건강기능식품 및 동물 사료 첨가제에 관한 것이다. 본 발명에 따르면 형질전환된 제브라피쉬로부터 인간 락토페린을 대량으로 쉽게 확보할 수 있고, 나아가 형질전환된 제브라피쉬 자체는 건강기능식품의 원료 및 동물 사료 첨가제로서 유용하게 사용할 수 있다.
Abstract translation: 本发明涉及在用含有人乳铁蛋白基因的重组表达载体转化后大规模生产人乳铁蛋白的方法，涉及从所述转基因斑马鱼大规模生产人乳铁蛋白的方法，以及含有所述转基因斑马鱼的功能性食品和动物饲料添加剂 转基因斑马鱼作为活性成分。 根据本发明，可以从转基因斑马鱼获得大量的人乳铁蛋白。 此外，转基因斑马鱼可用作功能性食品的成分，也可用作动物饲料的添加剂。
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6.发明公开인간 락토페린을 대량생산하기 위한 형질전환 제브라피쉬및 이를 이용한 인간 락토페린의 대량생산방법 无效用于生产人LACTOFERRIN的转基因青霉素和使用其生产人LACTOFERRIN的方法
公开(公告)号：KR1020100007241A
公开(公告)日：2010-01-22
申请号：KR1020080067786
申请日：2008-07-11
Applicant: 서울대학교산학협력단 , 주식회사 바이오노트
CPC classification number: C07K14/79 , A01K67/0278 , A01K2217/052 , A01K2227/40 , A01K2267/01
Abstract: PURPOSE: A transformed zebrafish massively producing human lactoferrin is provided to cheaply produce human lactoferrin and use lactoferrin as food, health food, and animal feed additive. CONSTITUTION: A transformed zebrafish which massively produces human lactoferrin is produced by transforming with a recombinant expression vector containing a human lactoferrin gene. The transformation is performed at fertilized egg of one or two cells. The transformation is performed through microinjection, electroporation, particle bombardment, sperm-mediated gene transfer, viral infection, direct muscle injection, insulator, or transposon. A method for massively producing the human lactoferrin comprises: a step of preparing the recombinant expression vector; a step of transforming the expression vector into the zebrafish fertilized egg; a step of culturing the transformed zebrafish; and a step of isolating and purifying human lactoferrin from the transformed zebrafish.
Abstract translation: 目的：提供大规模生产人乳铁蛋白的转化斑马鱼，以廉价生产人乳铁蛋白，并使用乳铁蛋白作为食品，保健食品和动物饲料添加剂。 构成：通过用含有人乳铁蛋白基因的重组表达载体转化，产生大量产生人乳铁蛋白的转化的斑马鱼。 在1或2个细胞的受精卵中进行转化。 通过显微注射，电穿孔，粒子轰击，精子介导的基因转移，病毒感染，直接肌肉注射，绝缘体或转座子进行转化。 一种大规模生产人乳铁蛋白的方法包括：制备重组表达载体的步骤; 将表达载体转化为斑马鱼受精卵的步骤; 培养转化的斑马鱼的一个步骤; 以及从转化的斑马鱼分离和纯化人乳铁蛋白的步骤。
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