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公开(公告)号：CA2772376A1
公开(公告)日：2011-03-03
申请号：CA2772376
申请日：2010-08-30
Applicant: BIO RAD LABORATORIES
Inventor： MCCOLLUM TOM , PATT PAUL , SHEN FRANK , CHU DANIEL Y , FLORY DON , GRIFFIN MIKE , HENG XIN , HEFNER ELI
Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2 x 2 µm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
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公开(公告)号：WO2012115979A9
公开(公告)日：2013-02-28
申请号：PCT/US2012025969
申请日：2012-02-21
Applicant: BIO RAD LABORATORIES , HENG XIN , PATT PAUL
IPC: G01N21/00
CPC classification number: G01N15/1459 , G01N15/1434 , G01N2015/144
Abstract: Systems and methods for performing cytometry using a linear light sensor. An illumination field, a line scanned by the linear light sensor, or both are swept across a cell to be imaged. Relative motion between the cell and the swept illumination may be created using a movable optical component or components, by adhering cells to a plate and transporting the plate or by other techniques.
Abstract translation: 使用线性光传感器进行细胞计数的系统和方法。 照明场，由线性光传感器扫描的线或两者都扫过要成像的细胞。 电池和扫描照明之间的相对运动可以通过使用可移动的光学部件或组件，通过将电池附着到板上并传送该板或通过其它技术来产生。
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公开(公告)号：WO2009009495A3
公开(公告)日：2009-05-14
申请号：PCT/US2008069351
申请日：2008-07-07
Applicant: BIO RAD LABORATORIES , PATT PAUL , TALACKI JOEL
Inventor： PATT PAUL , TALACKI JOEL
IPC: G01N21/00
CPC classification number: G01J1/4228 , G01J3/4406 , G01N21/645
Abstract: Systems and method for detecting and measuring light emitted from a sample and having a large dynamic range, e.g., a range of luminous intensity covering six or more orders of magnitude, that may be difficult to fully detect using a single detector with a limited detection range. Simultaneous measurement of the emitted light in two intensity ranges is performed using two detectors, e.g., one including a photomultiplier tube (PMT) and the other including a solid state detector such as a photodiode.
Abstract translation: 用于检测和测量从样品发射的光并具有大动态范围的系统和方法，例如覆盖六个或更多个数量级的发光强度的范围，这可能难以使用具有有限检测范围的单个检测器进行完全检测 。 使用两个检测器（例如包括光电倍增管（PMT）的两个检测器，另一个包括诸如光电二极管的固态检测器）进行同时测量两个强度范围内的发射光。
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公开(公告)号：WO2012071275A3
公开(公告)日：2012-07-26
申请号：PCT/US2011061425
申请日：2011-11-18
Applicant: BIO RAD LABORATORIES , ARCHER ROBERT M , HSIUNG FRANK , PATT PAUL
Inventor： ARCHER ROBERT M , HSIUNG FRANK , PATT PAUL
CPC classification number: G01N33/5094 , G01N15/0606 , G01N15/1463 , G01N2015/0288 , G01N2015/149
Abstract: Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.
Abstract translation: 通过对整个细胞群施加可转化的标记（无论是具有感兴趣特征还是未感兴趣特征的细胞），可以根据这些特征独立于它们的粘附状态对具有仅可检测到粘附状态的特征的粘附细胞进行分类， 处于其粘附状态并识别粘附表面上感兴趣细胞的位置。 然后选择性处理感兴趣的细胞或感兴趣的细胞以外的所有细胞以转化标记并实现感兴趣的细胞和剩余细胞之间的区分。 然后从贴壁状态释放所有细胞，并按照与非贴壁细胞相同的方式分类，但基于标记是否被转化或未转化。
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公开(公告)号：SG178555A1
公开(公告)日：2012-03-29
申请号：SG2012013009
申请日：2010-08-30
Applicant: BIO RAD LABORATORIES
Inventor： MCCOLLUM TOM , PATT PAUL , SHEN FRANK , CHU DANIEL Y , FLORY DON , GRIFFIN MIKE , HENG XIN , HEFNER ELI
Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2 x 2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
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Patent Agency Ranking
公开(公告)号：SG174439A1
公开(公告)日：2011-10-28
申请号：SG2011066958
申请日：2010-03-18
Applicant: BIO RAD LABORATORIES
Abstract: A system for performing high-speed, high-resolution imaging cytometry utilizes a line-scan sensor. A cell to be characterized is transported past a scan region. An optical system focuses an image of a portion of the scan region onto at least one linear light sensor, and repeated readings of light falling on the sensor are taken while a cell is transported though the scan region. The system may image cells directly, or may excite fluorescence in the cells and image the resulting light emitted from the cell by fluorescence. The system may provide a narrow band of illumination at the scan region. The system may include various filters and imaging optics that enable simultaneous multicolor fluorescence imaging cytometry. Multiple linear sensors may be provided, and images gathered by the individual sensors may be combined to construct an image having improved signal-to-noise characteristics.
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公开(公告)号：CA2818390C
公开(公告)日：2017-08-22
申请号：CA2818390
申请日：2011-11-18
Applicant: BIO-RAD LABORATORIES INC
Inventor： ARCHER ROBERT M , HSIUNG FRANK , PATT PAUL
Abstract: Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.
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公开(公告)号：CA2771324A1
公开(公告)日：2011-02-24
申请号：CA2771324
申请日：2010-08-20
Applicant: BIO RAD LABORATORIES
Inventor： PATT PAUL
IPC: G06K9/00
Abstract: A cross sectional imaging system performs high-resolution, high-speed partial imaging of cells. Such a system may provide much of the information available from full imaging cytometry, but can be performed much more quickly, in part because the data analysis is greatly reduced in comparison with full image cytometry. The system includes a light source and a lens that focuses light from the light source onto a small spot in a scanning location. A transport mechanism causes relative motion between a cell in the scanning location and the spot. A sensor generates a signal indicating the intensity of light emanating from the cell as a result of illumination by the light source. The system repeatedly takes readings of the light intensity signal and characterizes the light intensity along a substantially linear path across the cell.
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公开(公告)号：CA2772376C
公开(公告)日：2019-10-22
申请号：CA2772376
申请日：2010-08-30
Applicant: BIO RAD LABORATORIES
Inventor： MCCOLLUM TOM , PATT PAUL , SHEN FRANK , CHU DANIEL Y , FLORY DON , GRIFFIN MIKE , HENG XIN , HEFNER ELI
Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2 x 2 µm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
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公开(公告)号：CA2808499C
公开(公告)日：2017-06-13
申请号：CA2808499
申请日：2011-08-19
Applicant: BIO-RAD LABORATORIES INC
IPC: G01N15/10
Abstract: Flow cytometry system includes a flow element through which a cell is transported in a flowing fluid. Flow element includes a bore bounded by a wall. Light source is configured to illuminate the cell. Optical system receives light emanating from the cell and directs at least some of received light to a light sensor. The optical system includes a numerical-aperture-increasing lens at a wall of the flow element. At least some of the received light passes through the numerical-aperture-increasing lens. The flow cytometry system may also include a beam splitter that directs two wavelength bands of emanating light such that light in two wavelength band preferentially reach different sensing locations via different paths. The system may also include an optical element placed in one of the paths, shifting a focal location of the affected path to compensate for chromatic aberration of the numerical-aperture-increasing lens.
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