公开(公告)号：US4071766A

公开(公告)日：1978-01-31

申请号：US683832

申请日：1976-05-06

Applicant: Erika Kalman ,  Lajos Haklik ,  Anna Eke ,  Pal Kovacs

Inventor： Erika Kalman ,  Lajos Haklik ,  Anna Eke ,  Pal Kovacs

IPC: H01J37/20 ,  G01N21/00 ,  G21K5/06

CPC classification number: H01J37/20 ,  H01J2237/2003

Abstract: The invention relates to a micro-chamber for electron optical examinations, particularly for the in vitro and in vivo examinations of biologically active substances. The micro-chamber according to the invention comprises a casing provided with at least one entrance bore and optionally one or more exit bores, a specimen holder located in said casing, and a pressure-balancing buffer system attached to the micro-chamber.

Abstract translation: 本发明涉及用于电子光学检查的微室，特别涉及生物活性物质的体外和体内检查。 根据本发明的微室包括设置有至少一个入口孔和可选地一个或多个出口孔的壳体，位于所述壳体中的试样保持器和连接到微室的压力平衡缓冲系统。

公开(公告)号：US11961702B2

公开(公告)日：2024-04-16

申请号：US17546604

申请日：2021-12-09

Applicant: Saudi Arabian Oil Company

Inventor： Hassan Alqahtani ,  Dong Kyu Cha ,  Mohammed Al Otaibi

IPC: H01J37/26

CPC classification number: H01J37/261 ,  H01J2237/2003 ,  H01J2237/2802

Abstract: Systems and methods for preparing a nanofluidic LCTEM cell are provided. An exemplary method includes coating a photoresist layer onto a top surface of a silicon nitride substrate; etching channels into the photoresist layer; depositing calcite into the etched channels; removing the photoresist; placing the cell on a holder; connecting a first end of an inlet line to the cell; connecting a second end of the inlet line to an ultrasound transducer configured to generate nanobubbles; and connecting an outlet line to the cell.

公开(公告)号：US20230187170A1

公开(公告)日：2023-06-15

申请号：US17546604

申请日：2021-12-09

Applicant: Saudi Arabian Oil Company

Inventor： Hassan Alqahtani ,  Dong Kyu Cha ,  Mohammed Al Otaibi

IPC: H01J37/26

CPC classification number: H01J37/261 ,  H01J2237/2003 ,  H01J2237/2802

Abstract: Systems and methods for preparing a nanofluidic LCTEM cell are provided. An exemplary method includes coating a photoresist layer onto a top surface of a silicon nitride substrate; etching channels into the photoresist layer; depositing calcite into the etched channels; removing the photoresist; placing the cell on a holder; connecting a first end of an inlet line to the cell; connecting a second end of the inlet line to an ultrasound transducer configured to generate nanobubbles; and connecting an outlet line to the cell.

公开(公告)号：US20190080882A1

公开(公告)日：2019-03-14

申请号：US16185519

申请日：2018-11-09

Applicant: Protochips, Inc.

Inventor： Franklin Stampley Walden, II ,  John Damiano, JR. ,  Daniel Stephen Gardiner ,  David P. Nackashi ,  William Bradford Carpenter

IPC: H01J37/20

CPC classification number: H01J37/20 ,  H01J37/26 ,  H01J2237/2001 ,  H01J2237/2002 ,  H01J2237/2003

Abstract: A heating device having a heating element patterned into a robust MEMs substrate, wherein the heating element is electrically isolated from a fluid reservoir or bulk conductive sample, but close enough in proximity to an imagable window/area having the fluid or sample thereon, such that the sample is heated through conduction. The heating device can be used in a microscope sample holder, e.g., for SEM, TEM, STEM, X-ray synchrotron, scanning probe microscopy, and optical microscopy.

公开(公告)号：US20180330915A1

公开(公告)日：2018-11-15

申请号：US16025361

申请日：2018-07-02

Applicant: PROTOCHIPS, INC.

Inventor： Daniel Stephen Gardiner ,  Franklin Stampley Walden, II ,  John Damiano, JR.

IPC: H01J37/20 ,  H01J37/26

CPC classification number: H01J37/20 ,  H01J37/26 ,  H01J2237/2003 ,  H01J2237/204

Abstract: An electron microscope sample holder that includes at least one capillary having a sufficient inner diameter to act as a catheter pathway that allows objects that can be accommodated within the at least one capillary to be replaced or swapped with other objects. The sample holder having at least one capillary allows the user to insert and remove temporary fluidic pathways, sensors or other tools without the need to dissemble the holder.

公开(公告)号：US20180226221A1

公开(公告)日：2018-08-09

申请号：US15749900

申请日：2016-08-16

Applicant: Gerasimos Daniel DANILATOS

Inventor： Gerasimos Daniel DANILATOS

IPC: H01J37/28 ,  H01J37/147

CPC classification number: H01J37/28 ,  H01J37/1472 ,  H01J37/1474 ,  H01J37/20 ,  H01J2237/188 ,  H01J2237/2003 ,  H01J2237/2605 ,  H01J2237/2608 ,  H01J2237/2803 ,  H01J2237/2806 ,  H01J2237/2807 ,  H01J2237/2808 ,  H01J2237/2811

Abstract: Atmospheric scanning electron microscope achieves a wide field of view at low magnifications in a broad range of gaseous pressure, acceleration voltage and image resolution. This is based on the use of a reduced size pressure limiting aperture together with a scanning beam pivot point located at the small aperture at the end of electron optics column. A second aperture is located at the principal plane of the objective lens. Double deflection elements scan and rock the beam at a pivot point first at or near the principal plane of the lens while post-lens deflection means scan and rock the beam at a second pivot point at or near aperture at the end of the optics column. The aperture at the first pivot may act also as beam limiting aperture. In the alternative, with no beam limiting aperture at the principal plane, maximum amount of beam rays passes through the lens and with no post-lens deflection means, the beam is formed (limited) by a very small aperture at or near-and-below the final lens while the aperture skims a shifting portion of the wide beam, which is physically rocked with a pivot on the principal plane but with an apparent pivot point close and above the aperture, all of which result in a wide field of view on the examined specimen.

公开(公告)号：US09997330B2

公开(公告)日：2018-06-12

申请号：US15359781

申请日：2016-11-23

Applicant: Protochips, Inc.

Inventor： Daniel Stephen Gardiner ,  Franklin Stampley Walden, II ,  John Damiano, Jr.

IPC: H01J37/26

CPC classification number: H01J37/261 ,  B01L9/52 ,  B01L2200/0636 ,  F16J15/022 ,  F16J15/062 ,  F16J15/064 ,  F16J15/104 ,  H01J37/20 ,  H01J2237/2003 ,  H01J2237/2006 ,  H01J2237/2007

Abstract: A support for an electron microscope sample includes a body defining a void for receiving a first micro-electronic device, and a first gasket positioned about the first surface. The first gasket further defines an arm extending at an angle away from a horizontal extending through the first micro-electronic device. In operation, the first micro-electronic device is installed onto the first gasket and the arm engages an outer facing side of the first micro-electronic device to grip the first micro-electronic device.

公开(公告)号：US09812285B2

公开(公告)日：2017-11-07

申请号：US15218512

申请日：2016-07-25

Applicant: FEI Company

Inventor： Pleun Dona ,  Luigi Mele

IPC: H01J37/30 ,  G21K5/08 ,  G01N21/05 ,  H01J37/16 ,  H01J37/20

CPC classification number: H01J37/20 ,  H01J2237/022 ,  H01J2237/2003 ,  H01J2237/2605 ,  H01J2237/2802

Abstract: Presented is a holder assembly for cooperating with a nanoreactor and an electron microscope. The holder assembly has a distal end for holding the nanoreactor. The volume has a fluid inlet and outlet. The holder assembly has fluid supply and outlet tubes which in working are connected to the fluid inlet and outlet of the nanoreactor. In working, the connection between the fluid inlet and outlet and the respective supply and outlet tubes are sealed by sealing elements. The holder assembly has a recess which, when the nanoreactor is attached and the holder is inserted into the evacuated portion of an electron microscope, forms a sealed pre-vacuum volume between the holder and the nanoreactor, with the pre-vacuum volume being evacuated via a pre-vacuum channel such that any fluid leakage is pumped away and does not enter the evacuated part of the electron microscope.

公开(公告)号：US20170098526A1

公开(公告)日：2017-04-06

申请号：US15359781

申请日：2016-11-23

Applicant: Protochips, Inc.

Inventor： Daniel Stephen Gardiner ,  Franklin Stampley Walden, II ,  John Damiano, Jr.

IPC: H01J37/26

CPC classification number: H01J37/261 ,  B01L9/52 ,  B01L2200/0636 ,  F16J15/022 ,  F16J15/062 ,  F16J15/064 ,  F16J15/104 ,  H01J37/20 ,  H01J2237/2003 ,  H01J2237/2006 ,  H01J2237/2007

Abstract: A support for an electron microscope sample includes a body defining a void for receiving a first micro-electronic device, and a first gasket positioned about the first surface. The first gasket further defines an arm extending at an angle away from a horizontal extending through the first micro-electronic device. In operation, the first micro-electronic device is installed onto the first gasket and the arm engages an outer facing side of the first micro-electronic device to grip the first micro-electronic device.

公开(公告)号：US20160351374A1

公开(公告)日：2016-12-01

申请号：US15233176

申请日：2016-08-10

Applicant: University of Washington

Inventor： Liguo Wang

IPC: H01J37/16 ,  C23F4/00 ,  C23F1/40 ,  H01J37/26

CPC classification number: H01J37/16 ,  C23F1/40 ,  C23F4/00 ,  H01J37/20 ,  H01J37/26 ,  H01J37/261 ,  H01J2237/2001 ,  H01J2237/2002 ,  H01J2237/2003 ,  H01J2237/2004

Abstract: A grid assembly for cryo-electron microscopy may be fabricated using standard nanofabrication processes. The grid assembly may comprise two support members, each support member comprising a silicon substrate coated with an electron-transparent silicon nitride layer. These two support members are positioned together with the silicon nitride layers facing each other with a rigid spacer layer disposed therebetween. The rigid spacer layer defines one or more chambers in which a biological sample may be provided and fast frozen with a high degree of control of the ice thickness.

Abstract translation: 用于低温电子显微镜的栅格组件可以使用标准的纳米制造工艺制造。 栅格组件可以包括两个支撑构件，每个支撑构件包括涂覆有电子透明氮化硅层的硅衬底。 这两个支撑构件与氮化硅层一起定位，其间设置有刚性间隔层。 刚性间隔层限定一个或多个室，其中可以提供生物样品并以高度控制冰厚度快速冷冻。