公开(公告)号：US20220216047A1

公开(公告)日：2022-07-07

申请号：US17602000

申请日：2020-04-22

Applicant: THE TRUSTEES OF INDIANA UNIVERSITY

Inventor： David E. CLEMMER ,  Martin F. JARROLD ,  Tarick J. EL-BABA ,  Corinne A. LUTOMSKI

IPC: H01J49/16 ,  H01J49/00 ,  H01J49/04 ,  H01J49/26

Abstract: A method for analyzing charged particles may include generating, in or into an ion source region, charged particles from a sample of particles, causing the charged particles to enter a mass spectrometer from the ion source region at each of a plurality of differing physical and/or chemical conditions in a range of physical and/or chemical conditions in which the sample particles undergo structural changes, controlling the mass spectrometer to measure at least the charge magnitudes of the generated charged particles at each of the plurality of differing physical and/or chemical conditions, determining, with a processor, an average charge magnitude of the generated charged particles at each of the plurality of differing physical and/or chemical conditions based on the measured charge magnitudes, and determining, with the processor, an average charge magnitude profile over the range of physical and/or chemical conditions based on the determined average charge magnitudes.

公开(公告)号：US20230048598A1

公开(公告)日：2023-02-16

申请号：US17791981

申请日：2021-02-03

Applicant: THE TRUSTEES OF INDIANA UNIVERSITY

Inventor： Martin F. JARROLD ,  David E. CLEMMER ,  Benjamin E. DRAPER ,  Lauren F. BARNES

IPC: C12N7/00 ,  G01N33/68

Abstract: A method for reducing heterogeneity of a virus preparation may include generating virus ions from the virus preparation, repeatedly increasing at least one of a temperature and an incubation period at the increased temperature of at least one of the virus preparation and the generated virus ions, measuring mass-to-charge ratios and charge magnitudes of at least some of the generated virus ions at each increase of the at least one of the temperature and the incubation period, determining a mass spectrum at each increase of the at least one of the temperature and the incubation period based on values of the respective mass-to-charge ratios and charge magnitudes, and determining, based on the mass spectrums, optimum ones of the temperature and the incubation period which together minimize, or at least reduce, a heterogeneity of the virus preparation without aggregation of virus capsids in the virus preparation.

公开(公告)号：US20240242955A1

公开(公告)日：2024-07-18

申请号：US18436697

申请日：2024-02-08

Applicant: THE TRUSTEES OF INDIANA UNIVERSITY

Inventor： David E. CLEMMER ,  Martin F. JARROLD ,  Tarick J. EL-BABA ,  Corinne A. LUTOMSKI

IPC: H01J49/16 ,  H01J49/00 ,  H01J49/04 ,  H01J49/26

CPC classification number: H01J49/165 ,  H01J49/0031 ,  H01J49/0468 ,  H01J49/26

Abstract: A method for analyzing charged particles may include generating, in or into an ion source region, charged particles from a sample of particles, causing the charged particles to enter a mass spectrometer from the ion source region at each of a plurality of differing physical and/or chemical conditions in a range of physical and/or chemical conditions in which the sample particles undergo structural changes, controlling the mass spectrometer to measure at least the charge magnitudes of the generated charged particles at each of the plurality of differing physical and/or chemical conditions, determining, with a processor, an average charge magnitude of the generated charged particles at each of the plurality of differing physical and/or chemical conditions based on the measured charge magnitudes, and determining, with the processor, an average charge magnitude profile over the range of physical and/or chemical conditions based on the determined average charge magnitudes.

公开(公告)号：US20240050895A1

公开(公告)日：2024-02-15

申请号：US17766388

申请日：2020-10-09

Applicant: THE TRUSTEES OF INDIANA UNIVERSITY

Inventor： Brooke A. BROWN ,  David E. CLEMMER ,  Martin F. JARROLD

IPC: B01D59/46 ,  H01J49/26 ,  H01J3/40

CPC classification number: B01D59/46 ,  H01J49/26 ,  H01J3/40

Abstract: A method for purifying particles generates charged particles from a sample, measures at least at least one of masses, charge magnitudes and mobilities of the generated charged particles, and selectively passes to a particle collection target each of the measured charged particles having at least one of (a) a measured mass equal to a selected mass or within a selected range of particle masses, (b) a measured charge magnitude equal to a selected charge magnitude or within a selected range of charge magnitudes, (c) a mass-to-charge ratio equal to a selected mass-to-charge ratio or within a selected range of mass-to-charge ratios, and (d) a measured mobility equal to a selected mobility or within a selected range of mobilities. In some embodiments, the collected particles may be harvested and amplified.

公开(公告)号：US20230039701A1

公开(公告)日：2023-02-09

申请号：US17781485

申请日：2020-12-16

Applicant: THE TRUSTEES OF INDIANA UNIVERSITY

Inventor： Martin F. JARROLD ,  David E. CLEMMER

IPC: H01J49/42 ,  H01J49/06

Abstract: A charge filter instrument includes a field-free drift region, a plurality of charge detection cylinders in the drift region through which ions drifting axially therethrough pass, a plurality of charge sensitive amplifiers each coupled to at least one charge detection cylinder and configured to produce a charge detection signal corresponding to a charge of one or more of ions passing therethrough, a single inlet, single outlet charge deflector or a single inlet, multiple outlet charge steering device coupled to the outlet end of the drift region, means for determining charge magnitudes or charge states of ions drifting axially through the drift region based on the charge detection signals, and means for controlling the charge deflector or the charge steering device to pass through the single outlet or through a specified one of the multiple outlets only ions having a specified charge magnitude or charge state.

公开(公告)号：US20230013173A1

公开(公告)日：2023-01-19

申请号：US17781483

申请日：2020-12-16

Applicant: THE TRUSTEES OF INDIANA UNIVERSITY

Inventor： Martin F. JARROLD ,  David E. CLEMMER

IPC: H01J49/02 ,  H01J49/42 ,  H01J49/40 ,  H01J49/16

Abstract: A mass spectrometer may have an ion source region including an ion generator configured to generate ions from a sample, an ion detector configured to detect ions and produce corresponding ion detection signals, an electric field-free drift region disposed between the ion source region and the ion detector through which the generated ions drift axially toward the ion detector, a plurality of spaced-apart charge detection cylinders disposed in the drift region and through which the ions drifting axially through the drift region pass, and a plurality of charge amplifiers each coupled to a different one of the plurality of charge detection cylinders and each configured to produce a charge detection signal corresponding to a magnitude of charge of one or more of the generated ions passing through a respective one of the plurality of charge detection cylinders.