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公开(公告)号：KR1020090093292A
公开(公告)日：2009-09-02
申请号：KR1020080018738
申请日：2008-02-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: A bacteriolysis extract of Saccharomyces chevalieri is provided to increase the activation of neutrophil and enhance non-specific defense activity against the pathogenic bacteria. An immune enhancer, vaccine adjuvant, adjuvant treating agent or feed additive comprises bacteriolysis extract of Saccharomyces chevalieri as an active ingredient. A method for manufacturing a bacteriolysis extract of Saccharomyces chevalieri comprises: a step of culturing Saccharomyces chevalieri in media at 26°C for 72 hours; a step of centrifuging media and suspending in PBS; a step of adding dissolution agent and treating at room temperature for 2-3 days; a step of confirming the dissolution status with a microscope; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a refrigerator.
Abstract translation: 提供雪花酵母的溶菌提取物以增加嗜中性粒细胞的活化并增强针对致病菌的非特异性防御活性。 免疫增强剂，疫苗佐剂，佐剂处理剂或饲料添加剂包括作为活性成分的雪花酵母的溶菌提取物。 酿酒酵母的溶菌提取物的制备方法包括：在培养基中在26℃下培养雪花酵母（Saccharomyces chevalieri）72小时的步骤; 离心培养基并悬浮于PBS中的步骤; 加入溶解剂并在室温下处理2-3天的步骤; 用显微镜确认溶出状态的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在冰箱中干燥和保持的步骤。
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公开(公告)号：KR1020090091884A
公开(公告)日：2009-08-31
申请号：KR1020080017089
申请日：2008-02-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immune enhancer and vaccine adjuvant additive for animal, which contains Zygosaccharomyces bailii lysates and an auxiliary therapeutic agent are provided to enhance the activation of neutrophil and non-specific protection effect to attack inoculation. An immune enhancer, vaccine adjuvant additive, auxiliary therapeutic agent and feed additive comprises a Zygosaccharomyces bailii lysates. A method for manufacturing the Zygosaccharomyces bailii lysate comprises: a step of culturing the Zygosaccharomyces bailii in the media at 26‹C for 72 hours; a step of centrifuging the cultured media and suspending in phosphate buffered saline (PBS); a step of adding dissolution agent and stirring at room temperature for two to three days; a step of adjusting pH concentration to 6.5-7.2; and a step of drying and maintaining in a thermostatic chamber.
Abstract translation: 提供含有接合酵母发酵酵母裂解物和辅助治疗剂的动物免疫增强剂和疫苗佐剂添加剂，以增强中性粒细胞的活化和非特异性保护作用来攻击接种。 免疫增强剂，疫苗佐剂添加剂，辅助治疗剂和饲料添加剂包括接合酵母（Zygosaccharomyces bailii lysates）。 一种制造接合酵母发酵酵母裂解物的方法，包括：在培养基中培养接合酵母属酵母在26℃72小时的步骤; 将培养基离心并悬浮于磷酸盐缓冲盐水（PBS）中的步骤; 在室温下加入溶解剂并搅拌2〜3天的步骤; 将pH浓度调节至6.5-7.2的步骤; 以及在恒温室中干燥和保持的步骤。
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公开(公告)号：KR1020080068361A
公开(公告)日：2008-07-23
申请号：KR1020070006055
申请日：2007-01-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A plate agglutination tester and a method for measuring an immuno-reaction using the same are provided to decrease error of the result analysis in accordance with proficiency of a person performing diagnosis, show the degree of various immuno-reactions in an objective value and perform various statistical analyses, thereby being very useful for measuring the immuno-reaction. A plate agglutination tester consists of a transparent film and is characterized in that a section, which consists of a plurality of circles and where an antigen-antibody agglutination reaction occurs, is arranged and formed in horizontal and vertical directions on an upper side of the film. A method for measuring an immuno-reaction comprises the steps of: (a) mixing an antigen and an antibody specifically bound to the antigen with the plate agglutination tester to induce an agglutination reaction; (b) scanning the agglutination reaction induced plate agglutination tester and converting a scanning result into an image file; and (c) analyzing the obtained image file using a computer program capable of detecting fine agglutination reactants to numberize the intensity of the agglutination reaction. Further, the transparent film is an over head project film.
Abstract translation: 提供平板凝集试验机和使用其的免疫反应测定方法，根据进行诊断的人员的熟练程度来减少结果分析的误差，以目标值显示各种免疫反应的程度，并进行各种 统计分析，因此对测量免疫反应非常有用。 板凝集试验仪由透明膜构成，其特征在于，在膜的上侧，在水平和垂直方向上布置并形成由多个圆组成并且发生抗原 - 抗体凝集反应的部分 。 测定免疫反应的方法包括以下步骤：（a）将抗原与抗原特异性结合的抗体与平板凝集试验仪混合以诱导凝集反应; （b）扫描凝集反应诱导的板凝集测试仪并将扫描结果转换为图像文件; 和（c）使用能够检测细微凝集反应物的计算机程序来分析所获得的图像文件，以对凝集反应的强度进行编号。 此外，透明膜是顶部投影膜。
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公开(公告)号：KR100430934B1
公开(公告)日：2004-05-14
申请号：KR1020010082976
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在昆虫细胞和单克隆抗体中表达的重组3ABC非结构蛋白的口蹄疫诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒由 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。通过用重组杆状病毒感染昆虫细胞表达重组3ABC非结构蛋白。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入平板中; （2）使测试血清与平板反应; （3）使与试验血清中的口蹄疫病毒抗体结合的缀合物与测试血清具有酶，放射性物质或荧光物质; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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公开(公告)号：KR101201337B1
公开(公告)日：2012-11-15
申请号：KR1020100070745
申请日：2010-07-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 락토바실러스 존슨니(
Lactobacillus johnsonnii ) G22-2(KACC91458P), 락토바실러스 루테리(
Lactobacillus reuteri ) G8-5(KACC91450P) 및 락토바실러스 살리바리우스(
Lactobacillus salivarius ) G1-1(KACC91449P)로 이루어진 유산균 복합 균주를 유효성분으로 함유하는 사료첨가제에 관한 것이다.
본 발명의 유산균 복합 균주는 다양한 병원성 세균에 대한 항균력, 전분 분해능, 내산성, 내담즙성 및 장부착능이 우수하고 항생제 내성이 없어 안전하며 상호 억제 효과가 없어 사료첨가제로 사용하기에 적절하다. 본 발명의 유산균 복합 균주 사료첨가제는 여러 병원성 세균에 의한 감염으로부터 보호하고 사료의 이용 효율을 증대시킬 수 있을 뿐만 아니라 동물용 의약품을 대체할 수 있다.-
Patent Agency Ranking
公开(公告)号：KR101098946B1
公开(公告)日：2011-12-28
申请号：KR1020090066955
申请日：2009-07-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 내산성, 내담즙, 및 장부착능이 있고, 한국농업미생물지원센터(KACC)에 기탁번호 KACC91449P로 기탁된 균주인 락토바실러스 살리바리우스(
Lactobacillus salivarius ) G1-1 및 이를 유효성분으로 함유한 사료첨가제 조성물에 관한 것이다.
본 발명에 따르면, 본 발명의 락토바실러스 살리바리우스 G1-1 균주는 다양한 병원성 세균에 대해 우수한 항균활성을 나타냄은 물론 내산성 및 내담즙성 등이 우수하므로, 장내 병원성 세균 억제제, 소화제, 정장제 등의 프로바이오틱 조성물로 제조하여 사료 및 동물용 의약품에 유용하게 활용될 수 있고, 축산 농가의 가축질병으로 인한 경제적 피해를 크게 감소시킬 수 있을 뿐만 아니라 수입 동물용 의약품을 대체할 수 있다.
락토바실러스 살리바리우스, 항균활성, 내담즙성, 내산성-
公开(公告)号：KR100968069B1
公开(公告)日：2010-07-08
申请号：KR1020070107293
申请日：2007-10-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 우 결핵균 항체에 특이적으로 반응하는 단백질 및 그 정제 방법에 관한 것으로, 더욱 상세하게는 우 결핵균 단백질 중 우결핵균 감염 혈청에 대한 항체 특이 단백질 및 그 정제 방법에 관한 것이다. 본 발명은 우결핵균의 혈청학적 진단 기술을 향상시킴으로써 우결핵의 확산방지를 도모할 수 있다.
우결핵, 단백질항원, 이온교환크로마토그래피, 항체검사, 면역블롯팅-
8.发明公开
公开(公告)号：KR1020030052858A
公开(公告)日：2003-06-27
申请号：KR1020010082976
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N15/866 , C07K14/005 , C07K16/1009 , C07K2317/14 , C12N5/163 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在昆虫细胞中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组体3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒通过 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。重组体3ABC非结构蛋白用重组杆状病毒感染昆虫细胞表达。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白质导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，将稀释液倒入平板上; （2）使测试血清与板反应; （3）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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公开(公告)号：KR1020110009638A
公开(公告)日：2011-01-28
申请号：KR1020100070745
申请日：2010-07-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A23K10/18 , A23K20/195 , C12N1/20 , C12R1/225
Abstract: PURPOSE: A feed additive composition including novel lactobacillus species lactobacillus mixed bacterial strain is provided to offer the excellent antibacterial activity and starch resolution function for various pathogenic bacteria. CONSTITUTION: A feed additive composition contains a lactobacillus mixed bacterial strain formed with lactobacillus johnsonii G22-2, lactobacillus reuteri G8-5, and lactobacillus salivarius G1-1. The lactobacillus mixed bacterial strain has the antimicrobial activity against pathogenic bacteria, the starch degradation capability, the acid resistance, the bile tolerance, and the intestine adhesion capability.
Abstract translation: 目的：提供包含新型乳酸杆菌种乳杆菌混合菌株的饲料添加剂组合物，为各种病原菌提供优异的抗菌活性和淀粉分解功能。 构成：饲料添加剂组合物含有由约翰逊乳杆菌G22-2，乳酸杆菌G8-5和唾液乳杆菌G1-1形成的乳杆菌混合菌株。 乳杆菌混合菌株对病原菌具有抗菌活性，淀粉降解能力，耐酸性，胆汁耐受性和肠粘连能力。
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公开(公告)号：KR1020110009516A
公开(公告)日：2011-01-28
申请号：KR1020090066955
申请日：2009-07-22
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A feed additive composition containing Lactobacillus salivarius G1-1 is provided to ensure antibacterial activity to various pathogenic bacteria and to be used as a probiotic composition. CONSTITUTION: A Lactobacillus salivarius G1-1 is deposited in KACC91449P. The Lactobacillus salivarius G1-1 has 16s rDNA of sequence number 1 and is isolated from porcine small intestine by pure culture. A feed additive composition contains the Lactobacillus salivarius G1-1 as an active ingredient. The feed additive suppresses in vivo proliferation of pathogenic bacteria.
Abstract translation: 目的：提供含有唾液乳杆菌G1-1的饲料添加剂组合物，以确保对各种病原菌的抗菌活性，并用作益生菌组合物。 构成：唾液乳杆菌G1-1保藏在KACC91449P中。 唾液乳杆菌G1-1具有序列1的16s rDNA，通过纯培养从猪小肠中分离。 饲料添加剂组合物含有唾液乳杆菌G1-1作为活性成分。 饲料添加剂抑制致病菌的体内增殖。
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