公开(公告)号：SG166024A1

公开(公告)日：2010-11-29

申请号：SG2009028630

申请日：2005-05-02

Applicant: FLUIDIGM CORP

Inventor： GOODSAID FEDERICO ,  UNGER MARC A ,  HUANG JIANG ,  QUAN EMERSON ,  GROSSMAN ROBERT ,  LAM PHILLIP ,  CHOU HOU-PU ,  KIMBALL JAKE ,  PIEPRZYK MARTIN ,  FACER GEOFFREY

Abstract: An M x N matrix microfluidic device for performing a matrix of reactions, the device (100) having a plurality of reaction cells (106) in communication with one of either a sample inlet (120) or a reagent t inlet (124) through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method includes using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block. Fig. 1A

公开(公告)号：CA2734868A1

公开(公告)日：2010-03-11

申请号：CA2734868

申请日：2009-08-26

Applicant: FLUIDIGM CORP

Inventor： MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  UNGER MARC ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  C12N15/11

Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.

公开(公告)号：HK1107183A1

公开(公告)日：2008-03-28

申请号：HK07112600

申请日：2007-11-19

Applicant: FLUIDIGM CORP

Inventor： UNGER MARC A

IPC: B01L3/00 ,  B01L7/00 ,  C12Q20100101 ,  G01J20100101 ,  G01N20100101 ,  G01N27/447 ,  G01T20100101 ,  G02B21/02 ,  G02B21/16 ,  H01L20100101 ,  H05B20100101

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device (205). The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device (205). The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device (205). The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system (210, 212) coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

公开(公告)号：DE60120230T2

公开(公告)日：2007-03-29

申请号：DE60120230

申请日：2001-11-30

Applicant: FLUIDIGM CORP

Inventor： FERNANDES N ,  CHOU HOU-PU ,  UNGER A

IPC: F15C5/00 ,  B01L3/00 ,  F15C3/04 ,  F16K99/00 ,  G06F17/50

公开(公告)号：AU2001297572A8

公开(公告)日：2005-10-13

申请号：AU2001297572

申请日：2001-11-30

Applicant: FLUIDIGM CORP

Inventor： CHOU HOU-PU ,  UNGER MARC A ,  FERNANDES DAVID N

IPC: B01L3/00 ,  F15C3/04 ,  F15C5/00 ,  F16K99/00 ,  G06F17/50

公开(公告)号：AU2003224817A1

公开(公告)日：2003-10-20

申请号：AU2003224817

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM ,  CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

公开(公告)号：AU1189702A

公开(公告)日：2002-04-22

申请号：AU1189702

申请日：2001-10-12

Applicant: FLUIDIGM CORP

Inventor： MANAGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

IPC: A61L15/38 ,  A61L15/40 ,  B01L3/00 ,  B01L3/02 ,  B81B1/00 ,  B81C1/00 ,  G01N30/72 ,  H01J49/04

公开(公告)号：CA3018687C

公开(公告)日：2021-07-13

申请号：CA3018687

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  KAPER FIONA ,  MAY ANDREW ,  WANG JUN

IPC: G01N33/48 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/36 ,  C12Q1/6853

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：SG11201811048UA

公开(公告)日：2019-01-30

申请号：SG11201811048U

申请日：2017-07-12

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  FEKETE RICHARD A

IPC: C12Q1/68

公开(公告)号：SG10201807867XA

公开(公告)日：2018-10-30

申请号：SG10201807867X

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： JONES ROBERT C

Abstract: SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The kits, reagents, and methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids ( e.g., RNA) and proteins. FIG. 1