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    INTEGRATED SINGLE CELL SEQUENCING
    155.
    发明专利
    INTEGRATED SINGLE CELL SEQUENCING 未知

    公开(公告)号：SG10202004286UA

    公开(公告)日：2020-06-29

    申请号：SG10202004286U

    申请日：2015-05-08

    Applicant: FLUIDIGM CORP

    Inventor： WEST JASON A A ,  FOWLER BRIAN ,  CHARN TZE-HOWE ,  JOHNSON CHRISTIAN F ,  UNGER MARC A

    Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.

    ASSAY METHODS FOR INCREASED THROUGHPUT OF SAMPLES AND/OR TARGETS
    156.
    发明专利
    ASSAY METHODS FOR INCREASED THROUGHPUT OF SAMPLES AND/OR TARGETS 未知

    公开(公告)号：CA2734868C

    公开(公告)日：2019-09-10

    申请号：CA2734868

    申请日：2009-08-26

    Applicant: FLUIDIGM CORP

    Inventor： MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  UNGER MARC ,  ZIMMERMANN BERNHARD G

    IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6848 ,  C40B70/00

    Abstract: The present disclosure relates to methods for detecting a plurality of target nucleic acids in a plurality of samples comprising providing S samples that will be mixed together prior to assay, where S is an integer greater than 1; separately subjecting each of said S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence comprising a sample-specific nucleotide tag and a target nucleotide sequence; wherein T is the number of target nucleic acids to be detected, T being an integer greater than one; mixing together tagged target nucleotide sequences from said S samples to form an assay mixture; dividing the assay mixture into up to S × T amplification mixtures, and separately subjecting each of said amplification mixtures to amplification using a unique pair of amplification primers.

    СПОСОБ МУЛЬТИПРАЙМЕРНОЙ АМПЛИФИКАЦИИ ДЛЯ ШТРИХОВОГО КОДИРОВАНИЯ ЦЕЛЕВЫХ НУКЛЕИНОВЫХ КИСЛОТ
    157.
    发明专利
    СПОСОБ МУЛЬТИПРАЙМЕРНОЙ АМПЛИФИКАЦИИ ДЛЯ ШТРИХОВОГО КОДИРОВАНИЯ ЦЕЛЕВЫХ НУКЛЕИНОВЫХ КИСЛОТ 未知

    公开(公告)号：EA023190B9

    公开(公告)日：2018-03-30

    申请号：EA201171206

    申请日：2010-04-02

    Applicant: FLUIDIGM CORP

    Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

    IPC: C12Q1/68

    Abstract: Вопределенныхвариантахосуществленияданноеизобретениеобеспечиваетспособыамплификации, вкоторыхнуклеотиднуюметку(и) и, факультативно, штрих-кодовуюнуклеотиднуюпоследовательностьдобавляютк целевымнуклеотиднымпоследовательностям. Вдругихвариантахосуществленияданноеизобретениеобеспечиваетмикрофлюидноеустройство, котороевключаетмножествопервыхлинийвводаи множествовторыхлинийввода. Микрофлюидноеустройствотакжевключаетмножествонаборовпервыхкамери множествонабороввторыхкамер. Каждыйнаборпервыхкамернаходитсяв жидкостнойсвязис однимизмножествапервыхлинийввода. Каждыйнаборвторыхкамернаходитсяв жидкостнойсвязис однимизмножествавторыхлинийввода. Микрофлюидноеустройстводополнительновключаетмножествопервыхплунжерныхпарв жидкостнойсвязис первойчастьюмножествавторыхлинийвводаи множествовторыхплунжерныхпарв жидкостнойсвязис второйчастьюмножествавторыхлинийввода.

    Multi-primer amplification method for barcoding of target nucleic acids
    158.
    发明专利
    Multi-primer amplification method for barcoding of target nucleic acids 未知

    公开(公告)号：AU2010232439C1

    公开(公告)日：2017-07-13

    申请号：AU2010232439

    申请日：2010-04-02

    Applicant: FLUIDIGM CORP

    Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

    IPC: C12Q1/68

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    HIGH-LEVEL MULTIPLEX AMPLIFICATION
    159.
    发明专利
    HIGH-LEVEL MULTIPLEX AMPLIFICATION 未知

    公开(公告)号：CA3006994A1

    公开(公告)日：2017-06-22

    申请号：CA3006994

    申请日：2016-12-16

    Applicant: FLUIDIGM CORP

    Inventor： CHEN PEILIN

    IPC: C12Q1/68

    Abstract: The present disclosure provides a "looping amplification" method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    160.
    发明专利
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 未知

    公开(公告)号：SG10201608159XA

    公开(公告)日：2016-11-29

    申请号：SG10201608159X

    申请日：2013-02-28

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN ,  KIMBALL JAKE ,  MAUNG MYO THU ,  MAY ANDREW ,  NORRIS MICHAEL C ,  TOPPANI DOMINIQUE G ,  UNGER MARC A ,  WANG JING ,  WEST JASON A A

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

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