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公开(公告)号：KR100430935B1
公开(公告)日：2004-05-14
申请号：KR1020010082975
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组3ABC非结构蛋白由重组 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E.coli中表达的重组3ABC非结构蛋白制备杂交瘤细胞系3F-11（KCTC 10138BP） 将大肠杆菌导入动物体内，从动物体内收集免疫细胞，并将免疫细胞与癌细胞融合。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫诊断方法包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入板中; （2）使测试血清与平板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，与偶联物的荧光反应或辐射反应。
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公开(公告)号：KR1020030052859A
公开(公告)日：2003-06-27
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N5/163 , C07K16/1009 , C07K2317/14 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。通过克隆编码 SEQ ID NO：1的重组FMCV 2C非结构蛋白。通过用重组载体转化细胞来表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白引入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合，来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包衣缓冲溶液中稀释重组FMCV 2C非结构蛋白特异性单克隆抗体，将稀释物倒入板上; （2）洗涤板以除去未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与板反应; （4）洗涤板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与板反应; （6）洗板以除去未反应的检测血清; （7）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（8）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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13.发明公开대장균 발현 재조합 3ＡＢＣ 비구조단백질 및단클론항체를 이용한 구제역 진단방법 有权使用在大肠杆菌和单克隆抗体中表达的重组体3ABC非结构蛋白的FOOT-AND-MOUTH DISEASE的诊断方法
公开(公告)号：KR1020030052857A
公开(公告)日：2003-06-27
申请号：KR1020010082975
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C07K14/005 , C07K16/1009 , C07K2317/14 , C12N5/163 , C12N15/70 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫的诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组体3ABC非结构蛋白由重组体 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E中表达的重组3ABC非结构蛋白来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 将大肠杆菌转化成动物，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，并将稀释液倒在平板上; （2）使测试血清与板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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公开(公告)号：KR100634585B1
公开(公告)日：2006-10-16
申请号：KR1020040042854
申请日：2004-06-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/543
Abstract: 본 발명은 살모넬라 티피뮤리움(Salmonella typhimurium), 살모넬라 엠반다카(Salmonella mbandaka), 및 살모넬라 엔테라이티디스(Salmonella enteritidis)에서 추출한, 그룹특이성 및 민감성이 우수하고 항원성이 뛰어난 LPS(lipopolysaccharide)를 혼합하여 항원으로 사용하는 것을 특징으로 하는 동물의 살모넬라 감염항체 검사키트를 제공한다.
살모넬라 감염항체, 살모넬라 티피뮤리움, 살모넬라 엔테라이티디스, 살모넬라 엠반다카, 리포폴리사카라이드(LPS), ELISA, 검사키트-
公开(公告)号：KR100591660B1
公开(公告)日：2006-06-30
申请号：KR1020040025870
申请日：2004-04-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/112
CPC classification number: Y02A50/482
Abstract: 본 발명은 국내 유행 살모넬라균 혈청그룹인 그룹 B, C 및 D에 속하는 각각의 살모넬라균주의 세포외막단백질(OMP)을 면역항원으로 함유하는 동물 살모넬라 감염 예방용 혼합백신을 제공한다. 본 발명은 바람직하게는 상기 그룹 B에 속하는 살모넬라균으로는 살모넬라 티피뮤리움을, 그룹 C에 속하는 살모넬라균으로는 살모넬라 브렌더럽을, 및 그룹 D에 속하는 살모넬라균으로는 살모넬라 엔테라이티디스 균주가 선택되어지는 혼합백신을 제공한다.
Abstract translation: 本发明提供了一种家用方式沙门氏菌血清群B，C组和d中的每个动物为含有属于免疫抗原的沙门氏菌菌株的外膜蛋白（OMP）沙门氏菌感染的预防疫苗。 本发明优选在属于沙门氏菌的沙门氏菌细菌包括鼠伤寒沙门氏菌属于属于该组的B组，C组，沙门氏菌布兰德脏，和沙门氏菌d沙门氏菌entera ET放电应变被选择 ＆lt;
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Patent Agency Ranking
公开(公告)号：KR1020050100782A
公开(公告)日：2005-10-20
申请号：KR1020040025870
申请日：2004-04-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/112
CPC classification number: Y02A50/482 , A61K39/0275 , Y10S424/825 , Y10S424/826
Abstract: 본 발명은 국내 유행 살모넬라균 혈청그룹인 그룹 B, C 및 D에 속하는 각각의 살모넬라균주의 세포외막단백질(OMP)을 면역항원으로 함유하는 동물 살모넬라 감염 예방용 혼합백신을 제공한다. 본 발명은 바람직하게는 상기 그룹 B에 속하는 살모넬라균으로는 살모넬라 티피뮤리움을, 그룹 C에 속하는 살모넬라균으로는 살모넬라 브렌더럽을, 및 그룹 D에 속하는 살모넬라균으로는 살모넬라 엔테라이티디스 균주가 선택되어지는 혼합백신을 제공한다.
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17.发明授权
公开(公告)号：KR100430934B1
公开(公告)日：2004-05-14
申请号：KR1020010082976
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用在昆虫细胞和单克隆抗体中表达的重组3ABC非结构蛋白的口蹄疫诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒由 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。通过用重组杆状病毒感染昆虫细胞表达重组3ABC非结构蛋白。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入平板中; （2）使测试血清与平板反应; （3）使与试验血清中的口蹄疫病毒抗体结合的缀合物与测试血清具有酶，放射性物质或荧光物质; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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18.发明公开
公开(公告)号：KR1020040022247A
公开(公告)日：2004-03-12
申请号：KR1020010082714
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/12
Abstract: PURPOSE: A method of diagnosing vesicular stomatitis by performing an ELISA method using the recombinant envelope glycoprotein antigen of vesicular stomatitis virus(VSV) and monoclonal antibodies thereon is provided. Therefore, it permits quick, exact and specific antibody detection of the VSV and thus exact diagnosis of vesicular stomatitis through the detection. CONSTITUTION: The diagnosing method for vesicular stomatitis is achieved through a VSV detection method consisting of: attaching monoclonal antibodies against an envelope glycoprotein antigen of VSV on a solid supporter; binding the envelope glycoprotein antigen to the monoclonal antibodies attached on the solid supporter in the first step; reacting a sample containing an antibody against the envelope glycoprotein antigen with the envelope glycoprotein antigen of the second step; reacting a secondary antibody selected from the group consisting of a conjugate antibody attached with enzyme and a conjugate antibody attached with a luminescent material with the antibody of the third step.
Abstract translation: 目的：提供使用水泡性口炎病毒（VSV）的重组包膜糖蛋白抗原及其单克隆抗体进行ELISA方法诊断水泡性口炎的方法。 因此，它可以快速，精确和特异性地检测VSV，从而通过检测确定水泡性口炎的诊断。 构成：水泡性口炎的诊断方法是通过VSV检测方法实现的，该方法包括：将固定支持物上VSV的包膜糖蛋白抗原的单克隆抗体连接起来; 在第一步中将包膜糖蛋白抗原与附着在固体支持物上的单克隆抗体结合; 使含有针对包膜糖蛋白抗原的抗体的样品与第二步的包膜糖蛋白抗原反应; 使选自由酶附着的结合抗体和附着有发光材料的缀合物抗体的第二抗体与第三步骤的抗体反应。
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公开(公告)号：KR1020110009976A
公开(公告)日：2011-01-31
申请号：KR1020090067453
申请日：2009-07-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A monoclonal antibody for specifically identifying and detecting abnormal prion of chronic wasting disease is provided. CONSTITUTION: A fusion cell line of deposit number KCLRF-BP-00214 produces a monoclonal antibody for detecting abnormal prion of chronic wasting disease. A method for preparing fusion cell line comprises: a step of inoculating a peptide of sequence number 1 to knock-out mouse; a step of isolating B cells from the mouse; and a step of fusing the B cells with mouse myeloma cells. The monoclonal antibody is used for detecting the abnormal prion through western blotting.
Abstract translation: 目的：提供用于特异性鉴定和检测慢性消耗性疾病异常朊病毒的单克隆抗体。 构成：融合细胞系KCLRF-BP-00214产生一种用于检测慢性消耗性疾病异常朊病毒的单克隆抗体。 制备融合细胞系的方法包括：将序列号1的肽接种到敲除小鼠的步骤; 从小鼠中分离出B细胞的步骤; 以及将B细胞与小鼠骨髓瘤细胞融合的步骤。 单克隆抗体用于通过Western印迹检测异常朊病毒。
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公开(公告)号：KR1020100059035A
公开(公告)日：2010-06-04
申请号：KR1020080117663
申请日：2008-11-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A recombinant adenovirus expressing siRNA for suppressing and preventing foot-and-mouth virus is provided to generate siRNA targeting foot-and-mouth virus 2B and 3C genes and to ensure antiviral effect. CONSTITUTION: A recombinant adenovirus comprises a nucleotide of sequence number 3 or 5 and complementary nucleotide thereof(sequence number 4 or 6). An siRNA which specifically binds to the foot-and-mouth virus is synthesized from a DNA having a nucleotide of sequence number 1 or 2, or complementary nucleotide thereof. An antiviral formulation for foot-and-mouth contains the recombinant adenovirus expressing siRNA as an active ingredient.
Abstract translation: 目的：提供用于抑制和预防口蹄疫病毒的表达siRNA的重组腺病毒，以产生针对口蹄疫病毒2B和3C基因的siRNA，并确保抗病毒效果。 构成：重组腺病毒包含序列号3或5及其互补核苷酸（序列号4或6）的核苷酸。 特异性结合口蹄疫病毒的siRNA由序列号1或2的核苷酸或其互补核苷酸的DNA合成。 用于口蹄疫的抗病毒制剂含有表达siRNA作为活性成分的重组腺病毒。
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