公开(公告)号：WO2023069604A1

公开(公告)日：2023-04-27

申请号：PCT/US2022/047259

申请日：2022-10-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KONG, Say Li ,  LEOW, San Min ,  YING, Andy ,  FURTADO, Manohar R. ,  WONG, Grace Yu Hui ,  KENNY, Daryn ,  PHUA, Jacelyn Hong Yi ,  XU, Xiaohan

IPC: C12Q1/6851 ,  G16B40/10

Abstract: Disclosed are compositions, kits, and methods for amplifying and quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the quantification of a target nucleic acid from a sample using an internal quantification standard disposed in the same reaction volume as the target nucleic acid, thereby eliminating the need for additional amplification reactions and processing to generate a standard curve. Compositions, kits, and methods also enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.

公开(公告)号：WO2023064960A2

公开(公告)日：2023-04-20

申请号：PCT/US2022/078242

申请日：2022-10-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： SCHREIBER, Edgar, Hans

Abstract: Methods and systems are provided for genotyping a gene sequence. The method comprises obtaining first genotyping call data representing a query gene sequence. A numerical score is assigned to each of a plurality of allele calls of second genotyping call data by matching the first genotyping call data with the second genotyping call data, the second genotyping call data representing a plurality of candidate gene sequences. A match score is determined for each of the plurality of candidate gene sequences based on the numerical score assigned to each of the plurality of allele calls of the second genotyping call data, and a genotyping call is made for the query gene sequence based on a highest match score from among the match score determined for each of the plurality of candidate gene sequences.

公开(公告)号：WO2023044416A1

公开(公告)日：2023-03-23

申请号：PCT/US2022/076542

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： HANKS, Scott T. ,  KJAR, Steven R.

IPC: B01D19/00

Abstract: A bubble trap includes a body having an interior surface bounding a cavity that extends between an upper end and an opposing lower end, an axis extends centrally through the cavity of the body between the upper end and the lower end, a first plane extends through the body orthogonal to the axis so as to divide the body into an upper body portion and a lower body, the interior surface of the lower body portion having a constant first radius from a fixed first center point, the interior surface of the upper body portion not having a constant radius from a fixed center point. A tubular spout projects into the cavity at the lower end encircles a channel that communicates with the cavity. A liquid inlet port, a liquid outlet port, and a gas outlet port communicate with the cavity.

公开(公告)号：WO2023039598A1

公开(公告)日：2023-03-16

申请号：PCT/US2022/076352

申请日：2022-09-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHESNUT, Jonathan ,  IHLAN, Alicia ,  POTTER, Robert ,  SONG, Youngzee

IPC: A61K38/46 ,  C12N9/22 ,  C12N15/52 ,  C12N15/62 ,  C12N15/90

Abstract: The present disclosure relates to recombinant nucleases, recombinant nucleases operatively linked to nucleic acid binding domains, and methods of making and using them.

公开(公告)号：WO2023014729A1

公开(公告)日：2023-02-09

申请号：PCT/US2022/039194

申请日：2022-08-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KONG, Say Li ,  LEOW, San Min ,  YING, Andy ,  FURTADO, Manohar ,  WONG, Grace Yu Hui ,  KENNY, Daryn

IPC: C12Q1/6851 ,  C12Q1/70

Abstract: Disclosed are compositions, kits, and methods for quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels (using a standard curve) of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.

公开(公告)号：WO2023004400A1

公开(公告)日：2023-01-26

申请号：PCT/US2022/074028

申请日：2022-07-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LEVITZ, Andrew ,  MULLAH, Khairuzzaman Bashar ,  EVANS, Brian ,  BENSON, Scott C.

IPC: C09B11/24 ,  C09B1/00 ,  C09B11/28 ,  G01N33/542 ,  C09B56/00 ,  G01N21/64 ,  C09B67/22

Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described. Also disclosed is a method of detecting or quantifying a target nucleic acid molecule in a sample by polymerase chain reaction (PCR). The compounds have the following formula (I).

公开(公告)号：WO2022159874A1

公开(公告)日：2022-07-28

申请号：PCT/US2022/013665

申请日：2022-01-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BRZOSKA, Pius ,  WILDE, Joyce ,  FURTADO, Manohar ,  KENDRICK, Sara ,  ULEKLEIV, Camilla ,  STEVENS, Junko ,  VARMA, Kamini ,  HAYASHIBARA, Kathleen

IPC: C12Q1/70 ,  C12Q1/6888

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：WO2022155588A2

公开(公告)日：2022-07-21

申请号：PCT/US2022/012755

申请日：2022-01-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： SHANNON, Mark ,  ELDER, Noah ,  RAMAMOORTHI, Kalpith ,  LA, Jason ,  BLANCHARD, Daniel

IPC: C12Q1/6806

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the detection of target nucleic acids, including those from microbes and/or from infectious agents such as SARS-CoV-2 and other viruses. Embodiments described herein are designed to enable processing and analysis of the sample to detect target nucleic acids within the sample without requiring extraction and/or isolation of nucleic acid from the sample prior to subsequent processing steps. Samples analyzed can thus be "crude" biological samples that do not require pre-processing prior to placement in the workflow.

公开(公告)号：WO2022132667A1

公开(公告)日：2022-06-23

申请号：PCT/US2021/063156

申请日：2021-12-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WARD, Michael D. ,  KADUCHAK, Gregory ,  STRELKOV, Dmitriy

IPC: G01N15/14

Abstract: Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

公开(公告)号：WO2022126115A1

公开(公告)日：2022-06-16

申请号：PCT/US2021/072812

申请日：2021-12-08

Applicant: LIFE TECHNOLOGIES CORPORATION [US]/[US]

Inventor： GREENWOOD, John Paul

IPC: G01N30/20 ,  G01N30/60 ,  F16K11/085 ,  G01N30/38 ,  G01N30/46 ,  G01N35/10 ,  B01D15/18

Abstract: A chromatography system includes a first chromatography column and a panel having a top face and an opposing bottom face, a first cavity being formed on the panel so as to pass through the top face and be encircled by an inner surface. The panel bounds an inlet fluid channel having an end terminating at an inlet opening formed on the inner surface encircling the first cavity so that the inlet fluid channel communicates with the first cavity. The panel also bounds a plurality of first outlet fluid channels each having an end terminating at an outlet opening formed on the inner surface encircling the first cavity so that each of the plurality of first outlet fluid channels communicate with the first cavity, a first one of the plurality of first outlet fluid channels being in fluid communication with the first chromatography column. A first valve is rotatably disposed within the first cavity.