公开(公告)号：ES2385015T3

公开(公告)日：2012-07-17

申请号：ES07754529

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： RANK DAVID R ,  WEGENER JEFFERY ,  KORLACH JONAS ,  ROITMAN DANIEL ,  XU YUE ,  LYLE JOHN ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFF ,  CICERO RONALD L

IPC: C12N11/00 ,  C40B40/04

Abstract: Un método de proporcionar un sustrato que tiene una enzima inmovilizada selectivamente sobre él, comprendiendoel método:proporcionar un sustrato que tiene un primer componente de la superficie que comprende SiO2 y un segundocomponente de la superficie que comprende un metal u óxido metálico;poner en contacto el sustrato con una primera composición que se asocia selectivamente con el primer componentede la superficie, comprendiendo dicha primera composición un silano;acoplar la enzima a la primera composición; yponer en contacto el sustrato con una segunda composición que se asocia selectivamente con el segundocomponente de la superficie, comprendiendo dicha segunda composición un compuesto que comprende uno o másgrupos ácido fosfónico o fosfato.

公开(公告)号：AU2007261114B2

公开(公告)日：2012-07-12

申请号：AU2007261114

申请日：2007-06-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FOQUET MATHIEU ,  ROITMAN DANIEL BERNARDO ,  TURNER STEPHEN ,  PELUSO PAUL ,  OTTO GEOFFREY

IPC: C12M3/00 ,  G02B6/10

Abstract: Substrates, including zero mode waveguide substrates that have been fabricated to provide additional fυ&pgr;clionai elements and/or components including increased volumes for positioning of active surfaces and or components for the mitigation of negative electrochemical properties of the underlying substrates.

公开(公告)号：AT518010T

公开(公告)日：2011-08-15

申请号：AT06848008

申请日：2006-12-21

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： HANZEL DAVID ,  KORLACH JONAS ,  PELUSO PAUL ,  OTTO GEOFFREY ,  PHAM THANG ,  RANK DAVID ,  TURNER STEPHEN

IPC: C12Q1/68 ,  C12N11/14

Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

公开(公告)号：MX2010010600A

公开(公告)日：2011-03-30

申请号：MX2010010600

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  TRAVERS KEVIN ,  OTTO GEOFF ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/68

Abstract: Se describen composiciones y métodos para secuenciación de ácido nucleico que incluyen constructos de plantilla que comprenden porciones de doble hebra en constructos parcial o completamente contiguos, para proveer determinación de secuencia redundante por medio de una o ambas de secuenciación de hebras de sentido y anti-sentido y secuenciación iterativamente de todo el constructo múltiples veces. Componentes de secuencia adicionales son también incluidos opcionalmente dentro de tales constructos de plantilla. También se proveen métodos para el uso y preparación de estos constructor, también corno composiciones de secuenciación para su aplicación.

公开(公告)号：AU2009229157A1

公开(公告)日：2009-10-01

申请号：AU2009229157

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： MA CONGCONG ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  TRAVERS KEVIN

IPC: C12Q1/68

Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.

公开(公告)号：AU2009204461A1

公开(公告)日：2009-07-16

申请号：AU2009204461

申请日：2009-01-09

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  LYLE JOHN ,  LUONG KHAI ,  DALAL RAVI ,  LUNDQUIST PAUL

IPC: G01N21/64 ,  G01N21/00

Abstract: Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom.

公开(公告)号：CA2693979A1

公开(公告)日：2009-02-05

申请号：CA2693979

申请日：2008-07-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： CHRISTIANS FRED ,  TURNER STEPHEN

IPC: C12Q1/68 ,  C07H21/02

Abstract: Methods, systems and compositions where a target nucleic acid includes a registration sequence disposed therein for identification of the number or relative position of determined sequence from the template sequence. Particularly preferred aspects include a registration sequence in a circular template nucleic acid sequence which is, in turn, used in sequence by incorporation processes that rely upon template dependent, polymerase mediated primer extension in the identification of the sequence of the template.

公开(公告)号：AU2007261114A1

公开(公告)日：2007-12-27

申请号：AU2007261114

申请日：2007-06-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FOQUET MATHIEU ,  PELUSO PAUL ,  ROITMAN DANIEL BERNARDO ,  TURNER STEPHEN ,  OTTO GEOFFREY

IPC: C12M3/00 ,  G02B6/10

Abstract: Zero mode waveguide substrate (300) comprising an opaque cladding layer (304), a core (302) and a recess (308) forming an extension of the core volume into a transparent substrate (306) to provide increased volumes for positioning of active surfaces. The substrate (300) can be used in chemical and biochemical analysis.

公开(公告)号：CA2645758A1

公开(公告)日：2007-11-01

申请号：CA2645758

申请日：2007-03-29

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： OTTO GEOFF ,  WEGENER JEFFERY ,  KORLACH JONAS ,  RANK DAVID R ,  XU YUE ,  PELUSO PAUL ,  ROITMAN DANIEL ,  TURNER STEPHEN ,  LYLE JOHN

IPC: C12N11/00 ,  C40B40/04

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

公开(公告)号：AU2007215242A1

公开(公告)日：2007-08-23

申请号：AU2007215242

申请日：2007-02-09

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： TURNER STEPHEN ,  LACROIX YVES ,  FOQUET MATHIEU ,  LUNDQUIST PAUL ,  ZACCARIN DENIS ,  MAXHAM MARK

IPC: F21V9/16 ,  G01J1/58 ,  G01T1/10 ,  G21H3/02 ,  G21K5/00

Abstract: Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.