公开(公告)号：WO2019094524A1

公开(公告)日：2019-05-16

申请号：PCT/US2018/059710

申请日：2018-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： ROSENBAUM, Abraham ,  SEEGER, Collyn ,  GRAY, Jeremy ,  YU, Hua

IPC: C12Q1/6806 ,  C12Q1/6874

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/16 ,  C12Q2525/197 ,  C12Q2535/122 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/519

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：WO2019051316A1

公开(公告)日：2019-03-14

申请号：PCT/US2018/050088

申请日：2018-09-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHANG, Chi-Hsuan ,  LIANG, Xiquan

IPC: C12N15/63

CPC classification number: C12N15/63

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for introducing a nucleic acid cutting entity for DNA editing into cells that are difficult to transfect. Generally, the nucleic acid cutting entity is introduced into the cell without the use of viral vectors. The disclosure also relates to cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

公开(公告)号：WO2018231818A1

公开(公告)日：2018-12-20

申请号：PCT/US2018/037090

申请日：2018-06-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DHINGRA, Dalia ,  CHIEN, Richard

IPC: C12Q1/6806

Abstract: Provided are methods, as well as compositions, kits, and systems for preparing optimized control nucleic acids (polynucleotides) having reduce nucleic acid damage. Provided nucleic acid compositions provide reduced artifacts as compared to nucleic acid compositions prepared by conventional methods. Provided compositions are useful control in a variety of applications, including, but not limited to sequencing workflows to effectively monitor sensitivity, accuracy and/or precision of data.

公开(公告)号：WO2018157072A1

公开(公告)日：2018-08-30

申请号：PCT/US2018/019750

申请日：2018-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

Inventor： VARELA-ROHENA, Angel, M. ,  ANDOLINA, Melanie, B. ,  RILEY, James, L. ,  MEDVEC, Andrew

IPC: C12N5/0783

Abstract: This invention relates, inter alia , to compositions of low serum or serum free media and methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations.

公开(公告)号：WO2018156969A1

公开(公告)日：2018-08-30

申请号：PCT/US2018/019542

申请日：2018-02-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WANG, Shaohong

IPC: G01N27/447

Abstract: A system for separating biological molecules includes a plurality of capillaries (101), a capillary mount (102), a plurality of optical fibers (145a, 145b), a fiber mount (603), an optical detector (138), and a motion stage (606). The plurality of capillaries (101) are configured to separate biological molecules in a sample. Each capillary (101) comprising a detection portion (121) configured to pass electromagnetic radiation into the capillary (101). The plurality of capillaries (101) are coupled to the capillary mount (102) such that the detection portions (121) are fixedly located relative to one another. Each optical fiber (145) includes a receiving end to receive emissions. The optical fibers (145) are coupled to the fiber mount (603) such that the receiving ends of the optical fibers are fixedly located relative to one another. The optical detector (138) is configured to produce an alignment signal. The motion stage (606) is configured to align the receiving ends of the optical fibers (145) to the detection portions (121) based on values of the alignment signal.

公开(公告)号：WO2018136562A3

公开(公告)日：2018-07-26

申请号：PCT/US2018/014111

申请日：2018-01-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LOONEY, Timothy ,  LOWMAN, Geoffrey ,  LIN, Lifeng

IPC: C12Q1/6881 ,  C07K14/725 ,  C12Q1/6883

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：WO2017205796A3

公开(公告)日：2017-11-30

申请号：PCT/US2017/034776

申请日：2017-05-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAJUMDAR, Nivedita Sumi

IPC: G06F19/26

Abstract: In one exemplary embodiment, a method computer-implemented method of generating an outlier wheel data visualization for a graphical user interface, GUI, is provided. The method includes receiving emission data from a plurality of amplification reactions. The method further includes generating an outlier wheel data visualization using the emission data including a plurality of lines, where each line included in the outlier wheel data visualization represents emission data from an amplification reaction of the plurality of amplification reactions, and each line having a length and a visual indicator. The length of each line represents growth of intensity of fluorescence of the emission data and the visual indicator indicates the associated cycle number of the emission data. Further, each line is associated with an angular position so that the plurality of lines is configured in a circular shape. The method includes displaying the outlier wheel data visualization on a GUI.

公开(公告)号：WO2017197217A1

公开(公告)日：2017-11-16

申请号：PCT/US2017/032324

申请日：2017-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： IPPOLITO, Kim ,  PAULLIN, Jonathan ,  SALISBURY, Richard ,  STONE, Laurel ,  MELIOUS, Ethan ,  ZIEGENFUSS, Audra ,  PERSMARK, Erik

IPC: G02B21/36

Abstract: Provided are systems and methods that allow a user to capture images at low- and high-level magnification and then overlay the high-level magnification images on the low-level magnification image to ease review of the images. The high-level magnification images may be overlaid on the low-level magnification image based at least in part on the portion of the low-level magnification image from which the high-level image was originated.

Abstract translation: 提供允许用户以低和高等级放大率拍摄图像然后覆盖低等级放大率图像上的高等级放大率图像以便于查看图像的系统和方法 。 至少部分地基于低级别放大图像的源自高级别图像的部分，高级别放大图像可以被覆盖在低级别放大图像上。

公开(公告)号：WO2017116911A1

公开(公告)日：2017-07-06

申请号：PCT/US2016/068072

申请日：2016-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： COLLINS, Scott P. ,  RODRIGUEZ PERALTA, Federico Carols ,  JONES, Nephi D. ,  BRAU, Christopher D.

IPC: B01D11/02 ,  B01D11/04

CPC classification number: B01D11/02 ,  B01D11/0284 ,  B01D11/0446 ,  B01D11/0457 ,  B01D11/0488 ,  B01D11/0492

Abstract: Sample purification systems include a particle extraction assembly having a mixing compartment and a settling compartment. A biological sample is mixed with two liquid phases formulated to effectuate transfer of a biological molecule into a first phase and particulate contaminants into a second phase. The first phase includes a solubilizing salt, the second phase includes an organic molecule, and the mixture can have little or no monoatomic salt or dextran. The molecule-containing first phase can be optionally concentrated without also concentrating the particulate contaminants and introduced into a multi-stage liquid-liquid extractor, by which the biological molecule or molecular contaminants are extracted from the first phase into a third phase, thereby purifying the molecule away from contaminants. The extracted sample can be further purified through a series of processing steps. The system can be run in continuously mode to maintain sterility of the sample.

Abstract translation: 样品纯化系统包括具有混合室和沉降室的颗粒提取组件。 将生物样品与两个配制的液相混合以实现生物分子转移到第一相中，并将颗粒污染物转移到第二相中。 第一阶段包括增溶盐，第二阶段包括有机分子，并且混合物可以具有很少或不具有单原子盐或葡聚糖。 含有分子的第一相可以任选地浓缩而不浓缩颗粒污染物并引入到多级液 - 液萃取器中，由此将生物分子或分子污染物从第一相萃取到第三相中，从而纯化 分子远离污染物。 提取的样品可以通过一系列处理步骤进一步纯化。 系统可连续运行以保持样品的无菌性。

公开(公告)号：WO2017106783A1

公开(公告)日：2017-06-22

申请号：PCT/US2016/067374

申请日：2016-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PHELPS, Mwita ,  GULDE, Paul ,  FIKE, Richard ,  REYNOLDS, Mary ,  HASSETT, Richard ,  CAMPBELL, Andrew

IPC: C12N5/00 ,  C12N1/22

CPC classification number: C12N5/0018 ,  B01J8/1845 ,  C12N1/22 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/60

Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

Abstract translation: 本发明总体上涉及干细胞培养基或颗粒形式的饲料，其可以重构成液体培养基用于体外培养细胞。 每种丸粒组合物可以包含相同或不同的组合物; 例如不同的维生素，氨基酸，缓冲剂，微量盐，pH，铁螯合剂等。本发明还涉及通过改变不同丸粒组合物的比例来制备干细胞培养基的方法，或制备模块化干细胞培养物 培养基或使用颗粒定制培养细胞类型的培养基配方。 根据本发明，介质丸粒在重构之前，运输和处理过程中可能更容易处理; 和/或在重建期间。 培养基颗粒可用于任何容器中，如袋子，包括用于制备培养基制剂的无菌一次性袋子。 本发明还涉及使用培养基颗粒的试剂盒和培养系统。