公开(公告)号：DE3750094T2

公开(公告)日：1994-10-20

申请号：DE3750094

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：AT110739T

公开(公告)日：1994-09-15

申请号：AT89201371

申请日：1989-05-30

Applicant: SCLAVO SPA

Inventor： NENCIONI LUCIANO ,  PILERI PIERO ,  PEPPOLONI SAMUELE ,  SILVESTRI SERGIO

IPC: A61K39/00 ,  A61P37/00 ,  C07K5/02 ,  C07K16/00 ,  A61K39/39

公开(公告)号：ES2054701T3

公开(公告)日：1994-08-16

申请号：ES87830033

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：ES2052517T3

公开(公告)日：1994-07-16

申请号：ES87109318

申请日：1987-06-29

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： VERDINI ANTONIO SILVIO ,  BONELLI FABIO ,  PESSI ANTONELLO ,  CARDINALI FRANCO ,  BORASCHI DIANA ,  CENSINI STEFANO ,  DI TRAPANI ROMANO

IPC: A61K39/39 ,  A61K38/00 ,  A61K38/43 ,  A61P31/04 ,  A61P35/00 ,  A61P37/04 ,  C07C271/22 ,  C07K1/06 ,  C07K1/113 ,  C07K5/02 ,  C07K5/06 ,  C07K5/078 ,  C07K5/10 ,  C07K1/00 ,  A61K37/02

Abstract: New partially retro-inverted tuftsin analogues of general formula I wherein R represents the side-chain of the amino acids threonine, methionine or leucine R represents the side-chain of the amino acids lysine or arginine R is hydrogen or a metabolically labile acyl group, all the asymmetric carbon atoms are either of the S- or R-configuration, or, alternatively, the first, third, and fourth asymmetric carbons, starting from the N-terminal residue, are of the S-configuration while the second one is of the R- or (R,S)-configuration, and the corresponding pharmacologically acceptable salts, esters and amides. The new compounds which share the same pharmacological properties of tuftsin, are much more stable toward the enzymatic degradation than the parent molecule.

公开(公告)号：DE3889562D1

公开(公告)日：1994-06-23

申请号：DE3889562

申请日：1988-03-01

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： VELATI BELLINI ADA ,  TOMA SALVATORE ,  GRANDI GUIDO ,  COLLETTI ELISABETTA ,  RIBOLI BARBARA ,  COSMINA PAOLA ,  TOGNONI ANGELO ,  PEDRONI PAOLA ,  RAPPUOLI RINO

IPC: A61K39/10 ,  A61K39/00 ,  A61K39/07 ,  C07K14/235 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/70 ,  C12N15/75 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/04 ,  C12R1/07 ,  C12R1/125 ,  C12R1/19 ,  G01N33/569 ,  G01N33/577

公开(公告)号：DE69004900T2

公开(公告)日：1994-06-16

申请号：DE69004900

申请日：1990-08-27

Applicant: SCLAVO SPA

Inventor： VISCOMI GIUSEPPE CLAUDIO ,  CARDINALI FRANCO ,  LONGOBARDI MARIA GRAZIA

IPC: C07K1/14 ,  B01D15/42 ,  C07K1/18 ,  C07K5/02 ,  C07K5/06 ,  G01N30/02

Abstract: This invention firstly provides a method for purifying particular compounds of peptide or pseudo-peptide structure in which the number of protonable basic functions is greater than the number of acid functions and which have a molecular weight of less than 1000 daltons, by ion exchange displacement chromatography. In the method of the present invention the stationary phase used is a cationic exchange resin or a cross-linked polymer matrix activated with acid groups; the transporter solvent used is water if the compound to be purified already possesses at least one net positive charge, or aqueous dilute solutions of inorganic or strong organic acids which protonate the basic groups of the peptide or pseudo-peptide to be separated without modifying the structure of the peptide compound, such as acetic acid, trifluoroacetic acid, formic acid, hydrochloric acid or sulphuric acid; the displacer compound used is a triethylenetetraammonium salt.

公开(公告)号：AT105864T

公开(公告)日：1994-06-15

申请号：AT88830078

申请日：1988-03-01

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： VELATI BELLINI ADA ,  TOMA SALVATORE ,  GRANDI GUIDO ,  COLLETTI ELISABETTA ,  RIBOLI BARBARA ,  COSMINA PAOLA ,  TOGNONI ANGELO ,  PEDRONI PAOLA ,  RAPPUOLI RINO

IPC: A61K39/10 ,  A61K39/00 ,  A61K39/07 ,  C07K14/235 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/70 ,  C12N15/75 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/04 ,  C12R1/07 ,  C12R1/125 ,  C12R1/19 ,  G01N33/569 ,  G01N33/577

公开(公告)号：DE3884672T2

公开(公告)日：1994-04-28

申请号：DE3884672

申请日：1988-02-16

Applicant: SCLAVO SPA

Inventor： NENCIONI LUCIANO ,  ANTONI GUIDO ,  BORASCHI DIANA ,  TAGLIABUE ALDO

IPC: A61K38/00 ,  A61K39/39 ,  A61P37/04 ,  C07K14/545 ,  A61K37/02

Abstract: The specification relates to a synthetic peptide converted into a salt comprising one or more amine groups and capable of potentiating in vivo the primary and secondary antibody response to thymo-dependent and thymo-independent antigens having low immunogenicity. Injectable preparations comprising a solution of the peptide in a pharmacologically acceptable solvent are particularly useful as adjuvants in therapy.

公开(公告)号：DE3886118D1

公开(公告)日：1994-01-20

申请号：DE3886118

申请日：1988-07-13

Applicant: SCLAVO SPA

Inventor： PROTASI OTELLO ,  RAPPUOLI PAOLO

IPC: A61K38/00 ,  C07K14/565 ,  C12P21/02 ,  C07K3/20 ,  A61K37/66

Abstract: A method is described for the purification of crude human interferon from solutions containing it, which comprises: a) the complete adsorption of the crude interferon in a column of siliceous material which has previously been disinfected with an aqueous solution of formaldehyde; b) the washing of the column with non-pyrogenic, sterile, deionised water; c) the removal of the extraneous residual proteins by the elution of the column successively with a 1.4 M aqueous solution of NaCl, with non-pyrogenic, sterile, deionised water, and with an aqueous solution of a solvent having hydrophobic and acidic properties at a molar concentration of 0.001 M to 0.003 M; d) the elution of the interferon from the column with an aqueous solution of the same solvent as that used in step c) at a molar concentration of from 0.01 to 0.03 M and finally, e) the recovery and lyophilisation of the eluted fraction and/or fractions containing the purified interferon. The method enables interferon to be obtained, the purity of which, measured as its specific activity, is at least 1000 times greater than that of the crude interferon, with a yield greater than 85%. The interferon thus purified is particularly useful for treatment in man.

公开(公告)号：ITFI920146A1

公开(公告)日：1994-01-18

申请号：ITFI920146

申请日：1992-07-17

Applicant: SCLAVO SPA

Inventor： PRESENTINI RIVO ,  TERRANA BENEDETTO

IPC: C08G20060101 ,  C12N9/08 ,  G01N33/531 ,  G01N33/535 ,  G01N33/543