유전자 조작(접합)에 의한 환경정화 미생물 균주 및 그의 육종 방법
    5.
    发明授权
    유전자 조작(접합)에 의한 환경정화 미생물 균주 및 그의 육종 방법 失效
    通过遗传操作(接合)的环境净化微生物菌株及其育种方法

    公开(公告)号:KR1019890004029B1

    公开(公告)日:1989-10-16

    申请号:KR1019870014286

    申请日:1987-12-15

    Abstract: A method for breeding microorganisms containing multi-plasmid to degrade contaminants is presented. Thus, microorganism which can be used for naphthalene as a sole source of carbon is isolated from waste water and identified as Pseudomonas putida N3 (KCTC 8287 P). Pseudomonas putida CST3 (KCTC 8288P) containing TOL plasmid and CAM plasmid simultaneously is conjugated with KCTC 8287P strain to give a new strain Pseudomonas putida Conj 26 (KCTC 8289 P), which can degrade toluene, naphthalene, and camphor.

    Abstract translation: 提出了一种用于繁殖含有多质粒以降解污染物的微生物的方法。 因此,可用于萘作为唯一碳源的微生物从废水中分离出来,并被鉴定为恶臭假单胞菌N3(KCTC 8287 P)。 将含有TOL质粒和CAM质粒的恶臭假单胞菌CST3(KCTC 8288P)同时与KCTC 8287P菌株缀合,得到可以降解甲苯,萘和樟脑的新菌恶臭假单孢菌Conj 26(KCTC 8289 P)。

    신균주 바실러스 속(Bacillus sp.) NS-70(KCTC 8555P)와 이를 이용한 신규한 단백질 가수분해효소의 제조방법
    8.
    发明授权

    公开(公告)号:KR100127099B1

    公开(公告)日:1997-12-29

    申请号:KR1019940011237

    申请日:1994-05-24

    Abstract: The preparation method for the novel Bacillus sp. cell line NS-70 (KCTC 8555P) and the novel protease by using the bacillus sp. NS-70 includes the steps of: adding bacillus sp. cell line NS-70 (KCTC 8555P) to a culture containing polypeptone, yeast extract, beef extract, glycerol, KH2PO4, K2HPO4, MgSO4,7H2O and NaCl at pH 6 to 8 and fermenting the culture placed at 55 degrees centigrade for 6 hours in a culture containing phytonpeptone, K2HPO4, KH2HPO4, MgSO4,7H2O and NaCl at 50 degrees centigrade for 8-10 hours.

    Abstract translation: 新型芽孢杆菌的制备方法 细胞系NS-70(KCTC 8555P)和新型蛋白酶。 NS-70包括以下步骤:添加芽孢杆菌 细胞系NS-70(KCTC 8555P)在含有多胨,酵母提取物,牛肉提取物,甘油,KH 2 PO 4,K 2 HPO 4,MgSO 4·7H 2 O和NaCl的培养基中,pH6〜8,并将培养物放置在55℃下放置6小时 在50摄氏度下含有植物蛋白胨,K 2 HPO 4,KH 2 HPO 4,MgSO 4·7H 2 O和NaCl的培养物8-10小时。

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