公开(公告)号：CA2707600A1

公开(公告)日：2009-06-11

申请号：CA2707600

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： KORLACH JONAS ,  ROITMAN DANIEL ,  EID JOHN ,  OTTO GEOFF ,  HARDENBOL PAUL ,  FLUSBERG BENJAMIN

IPC: G01N33/573 ,  G01N33/53 ,  G01N33/535

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

公开(公告)号：CA2642937A1

公开(公告)日：2007-06-07

申请号：CA2642937

申请日：2006-12-01

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： EID JOHN ,  OTTO GEOFFREY ,  MURPHY DEVON ,  TURNER STEPHEN

IPC: A61K38/43 ,  A61K38/44

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.

公开(公告)号：AU2009297145A1

公开(公告)日：2010-04-01

申请号：AU2009297145

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

公开(公告)号：CA2707600C

公开(公告)日：2018-02-20

申请号：CA2707600

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： KORLACH JONAS ,  ROITMAN DANIEL ,  EID JOHN ,  OTTO GEOFF ,  HARDENBOL PAUL ,  FLUSBERG BENJAMIN

IPC: C12Q1/00 ,  C12M1/34 ,  C12M1/40 ,  G01N33/53 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

公开(公告)号：AU2006320414B2

公开(公告)日：2012-10-04

申请号：AU2006320414

申请日：2006-12-01

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： OTTO GEOFFREY ,  EID JOHN ,  TURNER STEPHEN ,  MURPHY DEVON

IPC: G01N33/53 ,  C12N11/02

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.

公开(公告)号：CA2738648A1

公开(公告)日：2010-04-01

申请号：CA2738648

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

公开(公告)号：CA2720247C

公开(公告)日：2020-07-14

申请号：CA2720247

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： EID JOHN ,  TURNER STEPHEN ,  DALAL RAVI ,  FLUSBERG BENJAMIN ,  KORLACH JONAS ,  LIN STEVEN ,  FEHR ADRIAN ,  CHRISTIANS FRED ,  EMIG ROBIN

IPC: G01N33/48 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2009251881B2

公开(公告)日：2015-03-26

申请号：AU2009251881

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  CHRISTIANS FRED ,  FEHR ADRIAN ,  FLUSBERG BENJAMIN ,  EMIG ROBIN ,  DALAL RAVI ,  KORLACH JONAS ,  LIN STEVEN ,  EID JOHN

IPC: G01N33/48 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2008331824B2

公开(公告)日：2014-07-24

申请号：AU2008331824

申请日：2008-12-03

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  EID JOHN ,  OTTO GEOFF ,  KORLACH JONAS ,  ROITMAN DANIEL ,  HARDENBOL PAUL

IPC: G01N33/53 ,  G01N33/535 ,  G01N33/573

Abstract: Systems and methods of enhancing fluorescent labeling strategies as well as systems and methods of using non-fluorescent and/or non-optic labeling strategies, e.g., as with single molecule sequencing using ZMWs, are described.

公开(公告)号：WO2010036311A3

公开(公告)日：2010-06-17

申请号：PCT/US2009005169

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

CPC classification number: C12Q1/6869 ,  G01N2021/7786 ,  Y10T436/143333 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2537/149 ,  C12Q2565/631 ,  C12Q2525/307 ,  C12Q2525/191

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

Abstract translation: 提供了用于在分析反应期间执行间歇检测的方法，装置和系统。 这种方法便于从不同的反应时间收集反应数据。 此外，此类方法可用于减少在给定反应时间下照明分析反应中一种或多种反应物的光致损伤。 在优选的实施方案中，使反应混合物经历至少一个照射和未照射的时间段并使其进行，使得照射反应混合物的时间小于光诱导的损伤阈值时间段。