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公开(公告)号：AU2012216605B2
公开(公告)日：2014-06-12
申请号：AU2012216605
申请日：2012-09-03
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： RANK DAVID R , WEGENER JEFFERY , KORLACH JONAS , ROITMAN DANIEL , XU YUE , LYLE JOHN , TURNER STEPHEN , PELUSO PAUL , OTTO GEOFF , CICERO RONALD L
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
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公开(公告)号：AU2007260707A1
公开(公告)日：2007-12-21
申请号：AU2007260707
申请日：2007-06-15
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： SHEN GENE , PELUSO PAUL , LYLE JOHN
IPC: C12Q1/68
Abstract: Controlled initiation of primer extension in determination of nucleic acid sequence information by incorporation of nucleotides or nucleotide analogs. Preferred aspects include photo-initiated extension through the use of photo-cleavable blocking, groups on termini of primer sequences followed by non-terminating primer extension using nucleotides or nucleotide analogs that are not extension terminators.
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3.发明申请
公开(公告)号：WO2012009206A2
公开(公告)日：2012-01-19
申请号：PCT/US2011043227
申请日：2011-07-07
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FEDOROV ANDREI , LYLE JOHN , BJORNSON KEITH , HANES JEREMIAH
Inventor： FEDOROV ANDREI , LYLE JOHN , BJORNSON KEITH , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12Q2527/125 , C12Q2563/137
Abstract: Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
Abstract translation: 公开了用于单分子核苷酸测序的组合物，试剂盒，方法和系统，其包括产生具有控制掺入的核苷酸的中值脉冲宽度的具有一价阳离子的聚合酶反应。 聚合中的锂，钠，钾，铷和铯等碱金属的含量被用于控制脉冲宽度，同时允许其它测序参数保持在期望的范围内。
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公开(公告)号：WO2010068289A3
公开(公告)日：2010-10-14
申请号：PCT/US2009006517
申请日：2009-12-10
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
Inventor： FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：CA2746632C
公开(公告)日：2020-06-30
申请号：CA2746632
申请日：2009-12-10
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH , PUGLISI JOSEPH
IPC: C12Q1/68 , C12Q1/6809 , C12Q1/6869
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
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Patent Agency Ranking
公开(公告)号：ES2385015T3
公开(公告)日：2012-07-17
申请号：ES07754529
申请日：2007-03-29
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： RANK DAVID R , WEGENER JEFFERY , KORLACH JONAS , ROITMAN DANIEL , XU YUE , LYLE JOHN , TURNER STEPHEN , PELUSO PAUL , OTTO GEOFF , CICERO RONALD L
Abstract: Un método de proporcionar un sustrato que tiene una enzima inmovilizada selectivamente sobre él, comprendiendoel método:proporcionar un sustrato que tiene un primer componente de la superficie que comprende SiO2 y un segundocomponente de la superficie que comprende un metal u óxido metálico;poner en contacto el sustrato con una primera composición que se asocia selectivamente con el primer componentede la superficie, comprendiendo dicha primera composición un silano;acoplar la enzima a la primera composición; yponer en contacto el sustrato con una segunda composición que se asocia selectivamente con el segundocomponente de la superficie, comprendiendo dicha segunda composición un compuesto que comprende uno o másgrupos ácido fosfónico o fosfato.
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公开(公告)号：AU2009204461A1
公开(公告)日：2009-07-16
申请号：AU2009204461
申请日：2009-01-09
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： TURNER STEPHEN , LYLE JOHN , LUONG KHAI , DALAL RAVI , LUNDQUIST PAUL
Abstract: Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom.
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公开(公告)号：CA2645758A1
公开(公告)日：2007-11-01
申请号：CA2645758
申请日：2007-03-29
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： OTTO GEOFF , WEGENER JEFFERY , KORLACH JONAS , RANK DAVID R , XU YUE , PELUSO PAUL , ROITMAN DANIEL , TURNER STEPHEN , LYLE JOHN
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
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公开(公告)号：AU2006330947A1
公开(公告)日：2007-07-05
申请号：AU2006330947
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： BIBILLO AREK , MITSIS PAUL , PHAM THANG , CHRISTIANS FRED C , MURPHY DEVON , PARK INSIL , HANZEL DAVID K , LYLE JOHN , RANK DAVID R , CLARK SONYA , PELUSO PAUL , OTTO GEOFF
IPC: C12Q1/68
Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
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公开(公告)号：AU2012216605A1
公开(公告)日：2012-09-20
申请号：AU2012216605
申请日：2012-09-03
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： RANK DAVID R , WEGENER JEFFERY , KORLACH JONAS , ROITMAN DANIEL , XU YUE , LYLE JOHN , TURNER STEPHEN , PELUSO PAUL , OTTO GEOFF
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
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