公开(公告)号：WO2013151647A1

公开(公告)日：2013-10-10

申请号：PCT/US2013/029072

申请日：2013-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： JI, Yongmei ,  BRZOSKA, Pius ,  BURRELL, Angela ,  CUMMINGS, Craig ,  O´CONNELL, Caytherine

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

Abstract translation: 公开了用于特异性和差异性检测鸟分枝杆菌亚种的组合物，测定法，方法，诊断方法，试剂盒和诊断试剂盒。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（例如土壤，污垢，垃圾，污水，空气或水）的样品中的副结核病，包括食品加工和制造表面或生物样品。

公开(公告)号：WO2013138724A2

公开(公告)日：2013-09-19

申请号：PCT/US2013/032107

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： PALLAS, Michael C. ,  NURSE, James ,  LIM, Gary ,  STRAUB, Theodore ,  FOSTER, Evan

IPC: B01L3/02

CPC classification number: G01N35/1002 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/0038 ,  B01J2219/00599 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0406 ,  C40B60/14

Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85 +/- 15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.

Abstract translation: 提供了一种用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。

公开(公告)号：WO2013109559A1

公开(公告)日：2013-07-25

申请号：PCT/US2013/021622

申请日：2013-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LI, Shifeng ,  BUSTILLO, James ,  HINZ, Wolfgang

IPC: G01N27/27 ,  B01L3/00 ,  C12M1/32 ,  G01N27/414 ,  G01N33/543

CPC classification number: G01N27/4148 ,  B01L3/5027 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0893 ,  G01N27/26 ,  G01N27/27 ,  G01N27/4145 ,  H01L21/302 ,  H01L21/31 ,  Y10T436/14 ,  Y10T436/142222 ,  Y10T436/143333

Abstract: A system includes a sensor including a sensor pad and includes a well wall structure defining a well operatively connected to the sensor pad. The sensor pad is associated with a lower surface of the well. The well wall structure defines an upper surface and a wall surface extending between the upper surface and the lower surface. The upper surface is defined by an upper buffer material having a high intrinsic buffer capacity of at least 2x 10 17 hydroxyl groups/m 2 at pH=7. The wall surface is defined by a wall material having a low intrinsic buffer capacity of not greater than 1.7x10 17 hydroxyl groups/m 2 at pH=7.

Abstract translation: 一种系统包括传感器，其包括传感器垫，并且包括限定可操作地连接到传感器垫的井的井壁结构。 传感器垫与井的下表面相关联。 井壁结构限定了在上表面和下表面之间延伸的上表面和壁表面。 上表面由在pH = 7时具有至少2×10 17个羟基/ m 2的高本征缓冲能力的上缓冲材料限定。 壁表面由在pH = 7时具有不大于1.7×10 17个羟基/ m 2的低本征缓冲能力的壁材料限定。

公开(公告)号：WO2013103883A1

公开(公告)日：2013-07-11

申请号：PCT/US2013/020372

申请日：2013-01-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIEU, Pauline

IPC: C12N5/0789

CPC classification number: C12N5/0696 ,  C12N2521/00

Abstract: Provided are methods useful in increasing efficiency of biotransport, reprogramming or altering a cell's state of differentiation, and maintenance of cells in an undifferentiated state.

Abstract translation: 提供的方法可用于提高生物传输，重编程或改变细胞分化状态和维持未分化状态的细胞的效率。

公开(公告)号：WO2013096906A1

公开(公告)日：2013-06-27

申请号：PCT/US2012/071482

申请日：2012-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： REARICK, Todd ,  BEAUCHEMIN, Mark

IPC: G01N27/414 ,  G06F19/22 ,  H03M7/30

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/27 ,  G01N27/4148 ,  G06F19/22

Abstract: A method, a computer program and an apparatus for measuring a waveform associated with a chemical event occurring on an ISFET sensor array. The waveform includes at least one region associated with expected measured values and at least one region associated with unpredictable measured values. First frame averaging is applied to at least one region associated with the expected measured values. Second frame averaging is applied to at least one region associated with the unpredictable measured values. The second number of frames used for averaging is less than the first number of frames. By the variable frame averaging the amount of stored data can be reduced.

Abstract translation: 一种用于测量与在ISFET传感器阵列上发生的化学事件相关联的波形的方法，计算机程序和装置。 波形包括与预期测量值相关联的至少一个区域和与不可预测的测量值相关联的至少一个区域。 将第一帧平均应用于与预期测量值相关联的至少一个区域。 将第二帧平均应用于与不可预测的测量值相关联的至少一个区域。 用于平均的第二帧数小于第一帧数。 通过可变帧平均化，可以减少存储的数据量。

公开(公告)号：WO2013095729A1

公开(公告)日：2013-06-27

申请号：PCT/US2012/055676

申请日：2012-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  SEMENOV, Eugene ,  GARGIR, Ari

Inventor： SEMENOV, Eugene ,  GARGIR, Ari

IPC: B01L3/00

CPC classification number: G01N1/28 ,  B01L3/5023 ,  B01L3/50273 ,  B01L2200/026 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0825 ,  B01L2400/0406

Abstract: Disclosed is a lateral flow capillary device (24) and uses thereof comprising a unipath capillary flow matrix (18) and a plurality of reservoirs ((32a)(32b)(32c)) each in fluid communication with the capillary flow matrix (18). The device includes one or more pressure delivery systems ((36a)(36b)(36c)(38a)(38b)(38c)) configured to urge capillary flow matrix (18) toward a portion of the housing (40) in which it resides, such that a substantially uniform pressure is applied over a portion of the capillary flow matrix (18).

Abstract translation: 公开了一种侧流毛细管装置（24）及其使用，其包括每个与毛细管流动基质（18）流体连通的单通道毛细管流动基质（18）和多个储存器（（32a）（32b）（32c））， 。 该装置包括一个或多个压力输送系统（（36a）（36b）（36c）（38b）（38c）（38c））（3​​8c）（38c）（38c）（38c）（38c）），其构造成将毛细管流动基质（18）推向壳体 使得在毛细管流动基质（18）的一部分上施加基本均匀的压力。

公开(公告)号：WO2013082518A1

公开(公告)日：2013-06-06

申请号：PCT/US2012/067427

申请日：2012-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： JONES, Meredith, Bauman ,  VASU, Sanjay ,  JUDD, David

IPC: B01D15/38 ,  G01N30/14

CPC classification number: G01N30/04 ,  B01D15/12 ,  B01D15/20 ,  B01D15/3828 ,  G01N2030/8831

Abstract: Compositions and methods for increasing protein recovery and/ or reducing metal ion stripping in IMAC chromatography or from resins used therein are provided. Samples like spent/ conditioned cell culture media or cell extracts containing native or recombinant proteins, were supplemented with metal ions before loading on to IMAC resins or columns to reduce metal ion stripping and/ or to increase protein recovery. The compositions and methods discussed herein work for a variety of metal affinity resins. Use of the compositions and methods disclosed may result in reusability of columns or resins, and improve the yield, purity and cost-effectiveness of using metal-ion based chromatography. The process is scalable to large-scale commercial levels.

Abstract translation: 提供了用于增加IMAC色谱中或从其中使用的树脂中的蛋白质回收和/或还原金属离子剥离的组合物和方法。 含有天然或重组蛋白质的废弃/调节细胞培养基或细胞提取物的样品在加载到IMAC树脂或柱之前补充金属离子以减少金属离子剥离和/或增加蛋白质回收。 本文讨论的组合物和方法适用于各种金属亲和树脂。 使用所公开的组合物和方法可以导致柱或树脂的可再利用性，并且提高使用基于金属离子的色谱法的产率，纯度和成本效益。 该过程可扩展到大规模的商业级别。

公开(公告)号：WO2013081864A1

公开(公告)日：2013-06-06

申请号：PCT/US2012/065650

申请日：2012-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

IPC: C12Q1/68

CPC classification number: C12Q1/6855 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

Abstract translation: 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 具体而言，提供了允许选择性扩增一个或多个靶序列的各种靶特异性引物。 一方面，本发明涉及用于选择性扩增与癌症或遗传疾病相关的一个或多个靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增的靶序列可用于各种下游过程，包括核酸测序并用于检测遗传变体的存在。

公开(公告)号：WO2013063230A1

公开(公告)日：2013-05-02

申请号：PCT/US2012/061858

申请日：2012-10-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： NURSE, James

IPC: G01N1/28 ,  B01L3/00

CPC classification number: C12Q1/6806 ,  B01L3/502707 ,  B01L3/5088 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2300/165

Abstract: The present invention relates to methods and apparatus for apportionment and manipulation of sample volumes into smaller discrete volumes. The method exploits the interplay of hydrophilic and hydrophobic forces to partition sample volumes. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed. The partition into extremely small volumes along with the device's inherent portability render our invention versatile for use in many areas, including but not limited to PCR, digital PCR, biological assays for diagnostics and prognostics, cancer diagnosis and prognosis, high throughput screening, single molecule and single cell reactions or assays, the study crystallization and other statistical processes, protein crystallization, drug screening, environmental testing, and the coupling to a wide range of analytical detection techniques for biomedical assays and measurements.

Abstract translation: 本发明涉及用于将样品体积分配和操纵成更小离散体积的方法和装置。 该方法利用亲水和疏水力的相互作用分配样品体积。 这些分隔的容积允许将样品和分离分离成可随后被操纵和分析的局部阵列。 分割成非常小的体积以及设备的固有可移植性使我们的发明具有多功能性，可用于许多领域，包括但不限于PCR，数字PCR，用于诊断和预后的生物测定，癌症诊断和预后，高通量筛选，单分子 和单细胞反应或测定，研究结晶和其他统计过程，蛋白质结晶，药物筛选，环境测试以及与广泛的用于生物医学测定和测量的分析检测技术的耦合。

公开(公告)号：WO2013059475A1

公开(公告)日：2013-04-25

申请号：PCT/US2012/060832

申请日：2012-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KORE, Anilkumar ,  MUTHIAN, Shanmugasundaram ,  GEE, Kyle

IPC: C07H21/02 ,  C12N15/67

CPC classification number: C12N15/67 ,  A61K31/7084 ,  A61K31/7088 ,  A61K31/7115 ,  A61K31/712 ,  A61K39/12 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55511 ,  C07C247/04 ,  C07C323/60 ,  C07D213/82 ,  C07H1/00 ,  C07H1/02 ,  C07H1/04 ,  C07H19/207 ,  C07H21/02 ,  C12N7/00 ,  C12N15/11 ,  C12N2770/36134 ,  C12N2770/36171 ,  C12P19/34 ,  C12Q1/68

Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The "click" modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the "click" reaction are useful for producing RNA transcripts and encoded protein.

Abstract translation: 提供炔基衍生的帽类似物，炔基修饰的封端RNA，1,4-二取代的三唑衍生的封端RNA，制备方法，分离方法及其用途。 “点击”修改有助于检测和分离封闭的RNA，通过“点击”反应形成的1,4-二取代的三唑衍生物可用于产生RNA转录物和编码的蛋白质。