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    MICROFLUIDIC DEVICES AND METHODS OF USING SAME
    131.
    发明专利
    MICROFLUIDIC DEVICES AND METHODS OF USING SAME 未知

    公开(公告)号：CA2807564C

    公开(公告)日：2014-01-28

    申请号：CA2807564

    申请日：2004-04-05

    Applicant: FLUIDIGM CORP

    Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

    IPC: B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  C40B30/00 ,  C40B60/00 ,  G01N33/48

    Abstract: A microfluidic device, with elastomeric components, and having a flow channel, wherein said flow channel has a plurality of blind flow channels opening into and in fluid communication with the flow channel, each blind flow channel having an aperture portion, a channel portion and a terminus portion, and wherein a region at the terminus of each blind flow channel defines a reaction site. Each blind flow channel is associated with a valve that when closed isolates the reaction site from the flow channel. The valves associated with each of the plurality of blind flow channels in fluid communication with the flow channel are under the control of a common control channel and are coordinately closed or opened.

    Copy number variation determination, methods and systems
    132.
    发明专利
    Copy number variation determination, methods and systems 未知

    公开(公告)号：IL204288A

    公开(公告)日：2013-06-27

    申请号：IL20428810

    申请日：2010-03-04

    Applicant: FLUIDIGM CORP

    Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME
    135.
    发明专利
    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME 未知

    公开(公告)号：SG187392A1

    公开(公告)日：2013-02-28

    申请号：SG2012094876

    申请日：2005-01-25

    Applicant: FLUIDIGM CORP

    Inventor： GROSSMAN ROBERT ,  UNGER MARC ,  LAM PHILLIP ,  CHOU HOU-PU ,  KIMBALL JAKE ,  PIEPRZYK MARTIN

    IPC: B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  C30B7/00 ,  C30B29/58

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one o f the ports, fluid pressure is changed therein.FIG. 14B

    PCR-free sample preparation and detection systems for high speed biologic analysis and identification
    136.
    发明专利
    PCR-free sample preparation and detection systems for high speed biologic analysis and identification 未知

    公开(公告)号：AU2007254191B2

    公开(公告)日：2013-01-10

    申请号：AU2007254191

    申请日：2007-05-16

    Applicant: FLUIDIGM CORP

    Inventor： SATTERFIELD BRENT COLEMAN ,  HUKARI KYLE WISDOM ,  WEST JASON ANDREW APPLETON ,  LI DUSTIN ,  MAJLOF LARS L

    IPC: B01L3/00 ,  C12N1/06 ,  C12Q1/68

    Abstract: Provided herein are biologic sample preparation and analysis systems that are rapid, portable, robust detection system for multiplexed detection of bio-threats, and which can be ruggedized to operate in harsh environments. A new method of detection called Combinatorial Probe Analysis (CPA), which provides an exponential increase in detection reliability, has been incorporated into these systems. This type of analysis greatly reduces false positives and false negatives; in addition it is reusable and eliminates special storage requirements for reagents. Specific technical advancements in the optimization of hybridization assays for nucleic acid detection on porous polymer monoliths (PPM) are also disclosed. Performing rapid and complete solubilization of viruses, vegetative bacteria and bacterial spores with an ultra high temperature solubilization protocol is also described. The systems provided herein provides the ability to perform rapid highly multiplexed analysis of a variety of bioagents, including bacteria viruses, and protein biotoxins. The systems and assays described herein are perform completely automated sample preparation and analysis, in a time frame of five minutes or less. The assay is simple in design allowing users in personal protective equipment to easily operate the system. The disclosed systems are robust, simple to use, and address the goals of the first responder community.

    NUCLEIC ACID ISOLATION METHODS
    137.
    发明专利
    NUCLEIC ACID ISOLATION METHODS 未知

    公开(公告)号：SG185544A1

    公开(公告)日：2012-12-28

    申请号：SG2012083432

    申请日：2011-05-16

    Applicant: FLUIDIGM CORP

    Inventor： MAY ANDREW ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  ZIMMERMANN BERNHARD

    Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

    SINGLE-CELL NUCLEIC ACID ANALYSIS
    139.
    发明专利
    SINGLE-CELL NUCLEIC ACID ANALYSIS 未知

    公开(公告)号：SG183029A1

    公开(公告)日：2012-08-30

    申请号：SG2012052031

    申请日：2010-01-13

    Applicant: FLUIDIGM CORP

    Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

    Abstract: SINGLE-CELL NUCLEIC ACID ANALYSISThe present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.Fig. 1

    SINGLE-CELL NUCLEIC ACID ANALYSIS
    140.
    发明专利
    SINGLE-CELL NUCLEIC ACID ANALYSIS 未知

    公开(公告)号：SG172965A1

    公开(公告)日：2011-08-29

    申请号：SG2011050739

    申请日：2010-01-13

    Applicant: FLUIDIGM CORP

    Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

    Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

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