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    구제역 바이러스의 감염여부를 진단하는 방법 및 이를구현하기 위한 진단키트
    13.
    发明公开
    구제역 바이러스의 감염여부를 진단하는 방법 및 이를구현하기 위한 진단키트 有权
    用于诊断国内动物脚部和口腔疾病的方法，包括反应部分，条带，试验样品引导部分和壳体的快速和诊断试剂盒

    公开(公告)号：KR1020040095824A

    公开(公告)日：2004-11-16

    申请号：KR1020030026809

    申请日：2003-04-28

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  프린스톤바이오메디테크코포레이션 ,  (주)피비엠이스트

    Inventor： 조인수 ,  현방훈 ,  이광녕 ,  엄재구 ,  계수정 ,  고영준 ,  구복경 ,  주이석 ,  안수환 ,  김인중 ,  김옥경 ,  김희정 ,  장기용 ,  신남규 ,  황서하 ,  강제모 ,  김창호 ,  고송우

    IPC: G01N33/543

    CPC classification number: G01N33/56983 ,  G01N33/526 ,  G01N33/558 ,  G01N2333/09 ,  Y10S436/811

    Abstract: PURPOSE: A method for diagnosis of foot and mouth disease and a diagnostic kit are provided, thereby rapidly and accurately diagnosing foot and mouth disease with a small amount of a sample, and easily and rapidly diagnosing foot and mouth disease of domestic animals in the field. CONSTITUTION: The method for diagnosis of foot and mouth disease comprises the steps of: (1) introducing a testing sample into a loading area of a strip; (2) combining a detecting reagent with a marker with a target material in the sample to prepare a complex; (3) developing the complex on a membrane; and (4) measuring the appearance change of a reaction portion having one or more fixing phase selected from antigen, antibody or hapten which is obtainable through the immune reaction from FMDV in a certain region of the membrane. The diagnostic kit comprises a reaction portion(13) having one or more fixing phase selected from antigen, antibody or hapten which is obtainable through the immune reaction from FMDV; a strip(1') having a control portion for determining normal operation on a certain region; a testing sample introducing portion; and a housing with a displaying window(4) for displaying reaction results in the reaction portion(13) and the control portion. on the strip(1').

    Abstract translation: 目的：提供口蹄疫诊断方法和诊断试剂盒，用少量样品快速，准确地诊断口蹄疫，容易快速诊断现场家畜的口蹄疫 。 规定：口蹄疫诊断方法包括以下步骤：（1）将检测样品引入条带的装载区域; （2）将检测试剂与标记物与样品中的靶材料组合以制备复合物; （3）在膜上显影复合物; 和（4）测量具有一个或多个定影相的反应部分的外观变化，所述固定相选自抗原，抗体或半抗原，所述抗原，抗体或半抗原可通过膜的某一区域中的FMDV通过免疫反应获得。 诊断试剂盒包括具有选自抗原，抗体或半抗原的一个或多个固定相的反应部分（13），其可通过FMDV的免疫反应获得; 具有用于确定某一区域正常操作的控制部分的条带（1'）; 测试样品引入部分; 以及具有用于在反应部（13）和控制部显示反应结果的显示窗（4）的壳体。 在条带（1'）上。

    재조합 광견병 바이러스, 이를 포함하는 광견병 예방용 백신 조성물 및 야외 광견병 바이러스와의 감별을 위한 멀티플렉스 RT-PCR
    14.
    发明公开
    재조합 광견병 바이러스, 이를 포함하는 광견병 예방용 백신 조성물 및 야외 광견병 바이러스와의 감별을 위한 멀티플렉스 RT-PCR 无效
    用于预防RABIES的重组病毒病毒和含有其的疫苗组合物，以及用于分化病毒性病毒的多重RT-PCR

    公开(公告)号：KR1020150138956A

    公开(公告)日：2015-12-11

    申请号：KR1020140066041

    申请日：2014-05-30

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 양동군 ,  나진주 ,  김하현 ,  현방훈 ,  김인중 ,  송재영

    IPC: C12N7/01 ,  A61K39/12 ,  C12N15/09 ,  C12Q1/68

    CPC classification number: C12N7/00

    Abstract: 본발명은재조합광견병바이러스, 이를포함하는광견병예방용백신조성물및 야외광견병바이러스와의감별을위한멀티플렉스 RT-PCR에관한것으로, 구체적으로광견병바이러스의 G 단백질에서 333번째아미노산이글루탐산으로치환되고 194번째아미노산이세린으로치환되어안정성과효과가우수해진재조합광견병바이러스, 이를포함하는광견병예방용백신조성물및 이재조합광견병바이러스와야외광견병바이러스를명확하게감별할수 있도록하는멀티플렉스 RT-PCR에관한것이다. 본발명의재조합광견병바이러스는 G 단백질의 333번째아미노산및 194번째아미노산이각각글루탐산과세린으로치환되어기존생백신주에비해매우안전하고, 경구투여용으로사용할수 있어기존광견병백신의문제점을해결할수 있다. 또한, 본발명의재조합광견병바이러스제조방법에따르면상기와같은우수한재조합광견병바이러스를숙주세포내에서효율적으로생산할수 있어, 대량생산을통해보다많이백신을보급함으로써광견병의예방에크게기여할수 있을것이라기대된다. 그리고본 발명의재조합광견병바이러스검출방법또는재조합광견병바이러스검출용키트를사용하면본 발명의재조합광견병바이러스와야외바이러스의감염여부를명확하게구별하여확인할수 있으므로, 백신이야생동물에게투여가되었는지혹은광견병바이러스에감염이되어광견병을유발할수 있는지의여부를용이하게판단할수 있다.

    Abstract translation: 本发明涉及重组狂犬病毒，包含其的重组狂犬病病毒疫苗组合物和用于检测重组狂犬病病毒的多重RT-PCR。 更具体地说，通过用谷氨酸代替狂犬病病毒的G蛋白中的第333位氨基酸，用丝氨酸替代第194位氨基酸来制备重组狂犬病病毒，以增强其效果和稳定性。 多重RT-PCR可用于准确识别室外狂犬病病毒。 重组狂犬病病毒是高度安全的，可用于口服给药以解决常规狂犬病疫苗的问题。 根据本发明，可以使用制备重组狂犬病病毒的方法，在宿主细胞中有效地产生优良的重组狂犬病病毒，通过批量生产分发疫苗，并有效预防疫苗。 根据本发明，可以使用重组狂犬病病毒检测方法或重组狂犬病病毒检测试剂盒来准确鉴定和区分重组狂犬病病毒的感染和重组狂犬病病毒的室外感染，以便容易地确定是否 疫苗被施用于野生动物，或者野生动物是否被感染并且能够诱导狂犬病感染。

    돼지 유행성설사병바이러스 중화 항체의 단쇄 가변 분절을 세균체 막표면에 발현하게 하는 벡터 및 돼지 유행성설사병 바이러스 증식을 억제하는 대장균
    17.
    发明公开
    돼지 유행성설사병바이러스 중화 항체의 단쇄 가변 분절을 세균체 막표면에 발현하게 하는 벡터 및 돼지 유행성설사병 바이러스 증식을 억제하는 대장균 有权
    转移载体，以表达单链抗变态反应片段，用于阻止细菌外膜上的PORCINE流行性腹膜病毒和E.COLI阻止PORCINE Epidemic DiarRHEA病毒的传播

    公开(公告)号：KR1020090011215A

    公开(公告)日：2009-02-02

    申请号：KR1020070074583

    申请日：2007-07-25

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 현방훈 ,  표현미 ,  김인중 ,  조수동 ,  김성희 ,  송재영

    IPC: C12N15/64 ,  C12N15/13 ,  C12N15/09

    Abstract: A vector and Escherichia coli suppressing proliferation of PED virus are provided to neutralize specifically the porcine epidemic diarrhea virus by being transformed to a surface expression vector and to reveal short chain variable segments of a porcine epidemic diarrhea virus neutralizing antibody at a film surface of a bacterial body. A surface expression vector reveals short chain variable segments of porcine epidemic diarrhea virus neutralizing antibody consisting of a sequence 1 at a film surface of a bacterial body. The surface expression vector includes a base sequence consisting of a sequence 1 and coding the short chain variable cleavage site specifically neutralized to porcine epidemic diarrhea virus(PEDV), T7 RNA polymerase consisting of a base sequence of 21bp~40bp of a sequence 4 and revealing the short chain variable split gene at a film surface of a bacterial body, a base sequence consisting of A sequence 2 coding A signal peptide of IgA proteinase gene of a gonococcus and an autotransporter beta domain gene consisting of a sequence 3 of the IgA proteinase of a base sequence coding six histidines and a gonococcus inserted into a bacterial membrane.

    Abstract translation: 提供抑制PED病毒增殖的载体和大肠杆菌，通过转化成表达表达载体来特异性中和猪流行性腹泻病毒，并显示在细菌膜表面的猪流行性腹泻病毒中和抗体的短链可变区段 身体。 表面表达载体揭示由细菌体的膜表面上的序列1组成的猪流行性腹泻病毒中和抗体的短链可变区段。 表面表达载体包括由序列1组成的碱基序列，并编码特异性中和到猪流行性腹泻病毒（PEDV）的短链可变切割位点，由序列4的21bp〜40bp的碱基序列组成的T7RNA聚合酶，并显示 在细菌体的膜表面的短链可变分裂基因，由编码淋球菌的IgA蛋白酶基因的A序列2的A序列组成的碱基序列和由IgA蛋白酶的序列3组成的自身转运蛋白β结构域基因 编码六个组氨酸的碱基序列和插入到细菌膜中的球菌属。

    돼지 유행성설사병 바이러스를 중화하는 특이 단클론 항체단쇄가변분절 유전자 및 이를 발현한 재조합단백질
    18.
    发明公开
    돼지 유행성설사병 바이러스를 중화하는 특이 단클론 항체단쇄가변분절 유전자 및 이를 발현한 재조합단백질 有权
    SCFV基因及其单克隆抗体2C10的重组蛋白中和病毒性腹膜病毒

    公开(公告)号：KR1020090011205A

    公开(公告)日：2009-02-02

    申请号：KR1020070074569

    申请日：2007-07-25

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 현방훈 ,  표현미 ,  김인중 ,  조수동 ,  김성희 ,  송재영 ,  권준헌

    IPC: C12N15/13 ,  C12N15/10 ,  C12N15/09

    Abstract: A gene comprising monoclonal antibody short chain variable segments neutralizing specifically porcine epidemic diarrhea virus(PEDV) is provided to prevent and treat PED pig disease. A gene comprising short chain variable segments neutralizes specifically porcine epidemic diarrhea virus(PEDV) consisting of a base sequence of a sequence 1. The gene comprising the short chain variable segments includes a heavy chain variable(VH) part consisting of a base sequence of 25~330 bp of the sequence 1 and a chain variable(VL) part consisting of a base sequence of 436~759 bp of the sequence 1. A monoclonal antibody includes a heavy chain variable(VH) part consisting of a base sequence of 25~330 bp of the sequence 1 and a chain variable(VL) part consisting of a base sequence of 436~759 bp of the sequence 1.

    Abstract translation: 提供包含中和特异性猪流行性腹泻病毒（PEDV）的单克隆抗体短链可变区的基因，以预防和治疗PED猪病。 包含短链可变区的基因特异性中和由序列1的碱基序列组成的猪流行性腹泻病毒（PEDV）。包含短链可变区的基因包括由25个碱基序列组成的重链可变（VH）部分 序列1〜330bp和由序列1的436〜759bp的碱基序列构成的链可变（VL）部分。单克隆抗体包括由25〜 序列1的330bp和由序列1的436〜759bp的碱基序列组成的链变量（VL）部分。

    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법
    19.
    发明公开
    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법 有权
    使用在INSEC细胞和单克隆抗体中表达的重组蛋白3ABC非结构蛋白的足球疾病的诊断方法

    公开(公告)号：KR1020030052858A

    公开(公告)日：2003-06-27

    申请号：KR1020010082976

    申请日：2001-12-21

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 권창희 ,  현방훈 ,  고영준 ,  이광녕 ,  구복경 ,  엄재구 ,  나진주 ,  남향미 ,  박지용 ,  계수정 ,  김인중 ,  이세영 ,  최광석 ,  손현주 ,  주이석

    IPC: C12N15/33

    CPC classification number: C12N15/866 ,  C07K14/005 ,  C07K16/1009 ,  C07K2317/14 ,  C12N5/163 ,  C12N2770/32131 ,  G01N33/56983 ,  G01N33/577 ,  G01N2333/09

    Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.

    Abstract translation: 目的：提供使用在昆虫细胞中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组体3ABC非结构蛋白的基​​因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒通过 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。重组体3ABC非结构蛋白用重组杆状病毒感染昆虫细胞表达。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白质导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，将稀释液倒入平板上; （2）使测试血清与板反应; （3）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。

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