아시네토박토 종 SY-01의 리파제 코딩 유전자 및이로부터 발현되는 리파제
    14.
    发明授权
    아시네토박토 종 SY-01의 리파제 코딩 유전자 및이로부터 발현되는 리파제 失效
    雅네토종종종SY-01파딩딩및및및종종종종종종종종종종종종

    公开(公告)号:KR100468547B1

    公开(公告)日:2005-01-29

    申请号:KR1020020045165

    申请日:2002-07-31

    Abstract: PURPOSE: Provided are a gene coding lipase of Acinetobacter species SY-01 with stereoselective substrate specificity and a protein expressed therefrom. CONSTITUTION: A gene is characterized by coding amino acid sequence of lipase of Acinetobacter species SY-01, represented by the SEQ ID NO:2. A transformed Bacillus subtilis 168/pSYLipSM(1) (KCTC 10267BP) is obtained by transformation with a recombinant vector plasmid pSYLipSM(1) containing the gene coding the lipase of Acinetobacter species SY-01. The lipase of Acinetobacter species SY-01 is manufactured by the steps of culturing the transformed bacillus subtilis, and isolating and purifying expressed lipase of Acinetobacter species SY-01 from the cultured cell.

    Abstract translation: 目的:提供具有立体选择性底物特异性的不动杆菌种SY-01的编码脂肪酶的基因和由其表达的蛋白质。 组成:基因的特征在于编码不动杆菌属物种SY-01的脂肪酶的氨基酸序列,由SEQ ID NO:2表示。 通过用含有编码不动杆菌属物种SY-01的脂肪酶的基因的重组载体质粒pSYLipSM(1)转化获得转化的枯草芽孢杆菌168 / pSYLipSM(1)(KCTC 10267BP)。 不动杆菌菌种SY-01的脂肪酶是通过培养转化的枯草芽孢杆菌,从培养细胞中分离纯化不动杆菌菌种SY-01的表达脂肪酶。

    써모액티노마이세스속(THERMOACTINOMYCES SP.) E79의 신규한이화대사물 조절 단백질 A 및 이를 코딩하는 DNA
    15.
    发明公开
    써모액티노마이세스속(THERMOACTINOMYCES SP.) E79의 신규한이화대사물 조절 단백질 A 및 이를 코딩하는 DNA 失效
    来自热休克霉素SP的新型CATABOLITE控制蛋白 E79和编码它的DNA

    公开(公告)号:KR1020000025930A

    公开(公告)日:2000-05-06

    申请号:KR1019980043230

    申请日:1998-10-15

    CPC classification number: C12N15/74 A61K38/00 C07K14/195

    Abstract: PURPOSE: A novel catabolism control protein(CcpA) and DNA encoding the same are provided which induce a mutation discharging a catabolism repression phenotype, so it can be useful to construct the mutant which produces a hydrolysis enzyme regardless of a sugar composite ion in a fermentative medium. CONSTITUTION: Provided are a CcpA, DNA sequence encoding the same and a strong promoter thereof which is an inhibitory factor of enzyme production. DNA encoding the CcpA is described by SEQ ID NO. 1 and purified from Thermoactinomyces sp.. The CcpA is concerned in a catabolism repression as an inhibitory factor of enzyme production and regulates the synthesis of protein hydrolysis enzyme.

    Abstract translation: 目的:提供一种新型的分解代谢控制蛋白(CcpA)和编码该DNA的DNA,其诱导排斥分解代谢阻遏表型的突变体,因此构建不产生糖复合离子的水解酶的突变体是有用的 中。 构成:提供了编码该酶的CcpA,DNA序列和作为酶生产的抑制因子的强启动子。 编码CcpA的DNA由SEQ ID NO: 1并从Thermoactinomyces sp。中纯化.CcpA涉及分解代谢抑制作为酶生产的抑制因子并调节蛋白质水解酶的合成。

    신균주 슈우도모나스 속(Pseudomonas sp.) NS-83(KCTC 8600P)와 이를 이용한 신규한 단백질 가수분해효소의 제조방법

    公开(公告)号:KR100127100B1

    公开(公告)日:1997-12-29

    申请号:KR1019940010758

    申请日:1994-05-17

    Abstract: PSEUDOMONAS SP. NS-83(KCTC 8600P) AND production of novel protein hydrolysis enzyme is disclosed in this invention. PSEUDOMONAS SP. NS-83(KCTC 8600P) is injected in PH6-8 of medium made by polypepton, extract of enzyme, extract of cow meat, glycol, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, cultivated for 6 hours at 35-40 degree Celsius. Thereafter, above-cultivated liquid is fermented in medium made by aqueous starch, phytonpepton, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, for 15 hours at 50 degree Celsius to produce protein hydrolysis enzyme having characteristics below; molecular weight : 32,000 dalton, optimum pH : 8.0, optimum temperature : 60deg.C.

    Abstract translation: PSEUDOMONAS SP。 NS-83(KCTC 8600P)和新型蛋白质水解酶的生产在本发明中公开。 PSEUDOMONAS SP。 将NS-83(KCTC 8600P)注射到由多肽,酶提取物,牛肉提取物,二醇,KH 2 PO 4,K 2 HPO 4,MgSO 4·7H 2 O和NaCl制成的培养基的PH6-8中,在35-40摄氏度下培养6小时 。 此后,将上述培养液体在含水淀粉,植物磷酸盐,KH 2 PO 4,K 2 HPO 4,MgSO 4·7H 2 O和NaCl的培养基中在50℃下发酵15小时以产生具有以下特征的蛋白质水解酶; 分子量:32,000道尔顿,最适pH:8.0,最适温度:60℃。

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