公开(公告)号：AU2009251881B2

公开(公告)日：2015-03-26

申请号：AU2009251881

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  CHRISTIANS FRED ,  FEHR ADRIAN ,  FLUSBERG BENJAMIN ,  EMIG ROBIN ,  DALAL RAVI ,  KORLACH JONAS ,  LIN STEVEN ,  EID JOHN

IPC: G01N33/48 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/52

Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided.

公开(公告)号：AU2009251883A1

公开(公告)日：2009-12-03

申请号：AU2009251883

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： BJORNSON KEITH ,  EMIG ROBIN ,  PELUSO PAUL ,  HE MOLLY ,  CLARK SONYA ,  BIBILLO AREK ,  LEE HAROLD ,  JIA LEI ,  CHRISTIANS FRED ,  PARK INSIL

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerase. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：ES2614078T3

公开(公告)日：2017-05-29

申请号：ES15168606

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

Abstract: Una composición que comprende una ADN polimerasa recombinante, ADN polimerasa recombinante que comprende una secuencia de aminoácidos que es al menos el 80 % idéntica a la SEQ ID NO: 1, ADN polimerasa recombinante que comprende un resto de ácido glutámico, un resto de ácido aspártico, un resto de histidina, un resto de lisina, un resto de arginina o un resto de tirosina en la posición A484, en la que la identificación de las posiciones es con respecto a la SEQ ID NO: 1, y ADN polimerasa recombinante que muestra actividad polimerasa.

公开(公告)号：AU2009251883B2

公开(公告)日：2014-09-11

申请号：AU2009251883

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： JIA LEI ,  BJORNSON KEITH ,  PARK INSIL ,  CLARK SONYA ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PELUSO PAUL ,  LEE HAROLD ,  BIBILLO AREK

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：AU2008282862A1

公开(公告)日：2009-02-05

申请号：AU2008282862

申请日：2008-07-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： CHRISTIANS FRED ,  TURNER STEPHEN

IPC: C12Q1/68 ,  C07H21/02

公开(公告)号：WO2011040971A3

公开(公告)日：2011-11-03

申请号：PCT/US2010002659

申请日：2010-09-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12N9/12 ,  C12N15/54 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Q2521/101

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

公开(公告)号：WO2009145820A2

公开(公告)日：2009-12-03

申请号：PCT/US2009001974

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

Inventor： CLARK SONYA ,  BIBILLO AREK ,  PELUSO PAUL ,  CHRISTIANS FRED ,  HE MOLLY ,  PARK INSIL ,  LEE HAROLD ,  BJORNSON KEITH ,  JIA LEI ,  EMIG ROBIN

IPC: C12N9/12 ,  C12N15/10 ,  C12N15/54

CPC classification number: C12N9/1252 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q2521/101

Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract translation: 提供了包含修饰的重组聚合酶的组合物，其表现出小于其衍生自其的聚合酶的支化部分的支化部分，或对于磷酸酯标记的核苷酸类似物的支化部分小于约25％。 还提供了包含修饰的重组聚合酶的组合物，其表现出相对于亲本聚合酶具有增加的稳定性的闭合的聚合酶/ DNA复合物。 还提供了包含修饰的重组聚合酶的组合物，其相对于亲本聚合酶表现出降低的速率常数。 提供了产生具有上述表型的聚合酶的方法。 提供使用这种聚合酶制备DNA或顺序DNA模板的方法。

公开(公告)号：WO2009017678A2

公开(公告)日：2009-02-05

申请号：PCT/US2008009064

申请日：2008-07-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  CHRISTIANS FRED ,  TURNER STEPHEN

Inventor： CHRISTIANS FRED ,  TURNER STEPHEN

IPC: C12Q1/68 ,  C07H21/02

CPC classification number: C12Q1/6869

Abstract: Methods, systems and compositions where a target nucleic acid includes a registration sequence disposed therein for identification of the number or relative position of determined sequence from the template sequence. Particularly preferred aspects include a registration sequence in a circular template nucleic acid sequence which is, in turn, used in sequence by incorporation processes that rely upon template dependent, polymerase mediated primer extension in the identification of the sequence of the template.

Abstract translation: 其中靶核酸包含置于其中的登记序列的方法，系统和组合物用于鉴定来自模板序列的确定序列的数目或相对位置。 特别优选的方面包括环形模板核酸序列中的配准序列，其依次通过依赖于模板依赖性，聚合酶介导的引物延伸在模板序列的鉴定中的掺入方法顺序使用。

公开(公告)号：EP2286217A4

公开(公告)日：2011-05-25

申请号：EP09755181

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： EID JOHN ,  TURNER STEPHEN ,  DALAL RAVI ,  FLUSBERG BENJAMIN ,  KORLACH JONAS ,  LIN STEVEN ,  FEHR ADRIAN ,  CHRISTIANS FRED ,  EMIG ROBIN

IPC: G01N33/48 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/52

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  C12N2310/50 ,  C12N2310/51 ,  C12Q1/6869 ,  C12Q2535/122 ,  C40B40/06 ,  G01N33/54313 ,  G01N33/54366

公开(公告)号：EP2274446A4

公开(公告)日：2011-04-20

申请号：EP09755191

申请日：2009-03-30

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： BJORNSON KEITH ,  LEE HAROLD ,  KORLACH JONAS ,  TURNER STEPHEN ,  BIBILLO AREK ,  CHRISTIANS FRED ,  EMIG ROBIN ,  HE MOLLY ,  PARK INSIL ,  JIA LEI ,  FEDOROV ANDREI ,  LYLE JOHN M

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/48

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2527/125 ,  C12Q2527/119 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2527/137 ,  C12Q2521/101