公开(公告)号：CA2991818A1

公开(公告)日：2009-10-01

申请号：CA2991818

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/6869 ,  G16B30/00 ,  C12Q1/6806 ,  G16B30/10

Abstract: Compositions for nucleic acid sequencing are provided that include template constructs that comprise double stranded portions in partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.

公开(公告)号：CA2746632C

公开(公告)日：2020-06-30

申请号：CA2746632

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6869

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：AU2008307617B2

公开(公告)日：2013-05-23

申请号：AU2008307617

申请日：2008-09-26

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TRAVERS KEVIN ,  TURNER STEPHEN ,  HEINER CHERYL

IPC: C12Q1/68 ,  C07H21/04

Abstract: Methods of preparing nucleic acid templates and providing the nucleic acid templates to low copy number reaction volumes are provided. Related compositions of nucleic acid templates are also provided.

公开(公告)号：MX2010010600A

公开(公告)日：2011-03-30

申请号：MX2010010600

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： TURNER STEPHEN ,  TRAVERS KEVIN ,  OTTO GEOFF ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/68

Abstract: Se describen composiciones y métodos para secuenciación de ácido nucleico que incluyen constructos de plantilla que comprenden porciones de doble hebra en constructos parcial o completamente contiguos, para proveer determinación de secuencia redundante por medio de una o ambas de secuenciación de hebras de sentido y anti-sentido y secuenciación iterativamente de todo el constructo múltiples veces. Componentes de secuencia adicionales son también incluidos opcionalmente dentro de tales constructos de plantilla. También se proveen métodos para el uso y preparación de estos constructor, también corno composiciones de secuenciación para su aplicación.

公开(公告)号：AU2009297145A1

公开(公告)日：2010-04-01

申请号：AU2009297145

申请日：2009-09-16

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MAXHAM KENNETH MARK ,  SORENSON JON ,  EID JOHN ,  MARKS PATRICK ,  TRAVERS KEVIN ,  GRAY DONALD ,  EMIG ROBIN ,  CHAISSON MARK ,  FLUSBERG BENJAMIN

IPC: G01N21/00

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

公开(公告)号：AU2009229157A1

公开(公告)日：2009-10-01

申请号：AU2009229157

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： MA CONGCONG ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  TRAVERS KEVIN

IPC: C12Q1/68

Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.

公开(公告)号：AU2009325069B2

公开(公告)日：2015-03-19

申请号：AU2009325069

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2817587A1

公开(公告)日：2012-05-18

申请号：CA2817587

申请日：2011-11-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FLUSBERG BENJAMIN ,  KORLACH JONAS ,  WEGENER JEFFREY ,  CLARK TYSON A ,  VILFAN IGOR ,  KISLYUK ANDREY ,  TURNER STEPHEN ,  SORENSON JON ,  TRAVERS KEVIN ,  HEINER CHERYL ,  TOMANEY AUSTIN B ,  MARKS PATRICK ,  LEE JESSICA ,  JIA LEI ,  WEBSTER DALE ,  LYLE JOHN ,  HANES JEREMIAH ,  PUGLISI JOSEPH

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2746632A1

公开(公告)日：2010-06-17

申请号：CA2746632

申请日：2009-12-10

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： FLUSBERG BENJAMIN ,  TURNER STEPHEN ,  LEE JESSICA ,  JIA LEI ,  KORLACH JONAS ,  SORENSON JON ,  WEBSTER DALE ,  LYLE JOHN ,  TRAVERS KEVIN ,  HANES JEREMIAH

IPC: C12Q1/68 ,  C12N15/10

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

公开(公告)号：CA2719747A1

公开(公告)日：2009-10-01

申请号：CA2719747

申请日：2009-03-27

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： TRAVERS KEVIN ,  OTTO GEOFF ,  TURNER STEPHEN ,  HEINER CHERYL ,  MA CONGCONG

IPC: C12Q1/68

Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.