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公开(公告)号：KR101617142B1
公开(公告)日：2016-05-02
申请号：KR1020140060752
申请日：2014-05-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 인트론바이오테크놀로지
Abstract: 본발명은개선된검출정확성을제공해줄 수있는핵산검사기반의조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트에관한것이다. 본발명에따른조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트는조류인플루엔자바이러스의목적유전자에서의짧은보존서열로인한문제점을극복할수 있는서열번호 1 내지서열번호 16으로특징지어지는특이프라이머들이적용되고, 또한기존조류인플루엔자바이러스검출용다중유전자증폭에서의민감도저하및 위음성발생문제를회피하기위하여 8개의튜브로구성된다중튜브방식이적용된것을특징으로한다. 본발명의조류인플루엔자바이러스검출및 H5형, H7형및 H9형을동시감별검출키트는조류인플루엔자바이러스검출과임상적으로주요한조류인플루엔자바이러스 H5형, 조류인플루엔자 H7형및 조류인플루엔자 H9형의동시감별검출에있어유용하게활용될수 있다.
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22.发明公开
公开(公告)号：KR1020140034342A
公开(公告)日：2014-03-20
申请号：KR1020120094251
申请日：2012-08-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K38/162 , A61K9/0019 , C07K14/01 , Y10S424/813 , Y10S514/867
Abstract: The present invention relates to a vaccine composition comprising domestic predominant epidemic genotype strains of bovine viral diarrhea virus for preventing or treating bovine viral diarrhea. When preparing a vaccine by mixing GB44-1 strain, GB45-1 strain, and 08Q723 strain which are the bovine viral diarrhea virus according to the present invention, the vaccine can be valuably used as an effective vaccine for preventing or treating the bovine viral diarrhea since the vaccine is suitable for domestic genotypes and has an outstanding immune function compared to a conventional vaccine, and solves a problem of a conventional vaccine for the bovine viral diarrhea.
Abstract translation: 本发明涉及一种疫苗组合物，其包含用于预防或治疗牛病毒性腹泻的牛病毒性腹泻病毒的国内主要流行基因型菌株。 通过混合根据本发明的作为牛病毒性腹泻病毒的GB44-1菌株，GB45-1菌株和08Q723菌株制备疫苗时，该疫苗可以有价值地用作预防或治疗牛病毒性腹泻的有效疫苗 因为该疫苗适用于国内基因型，并且与常规疫苗相比具有优异的免疫功能，并且解决了用于牛病毒性腹泻的常规疫苗的问题。
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公开(公告)号：KR101329349B1
公开(公告)日：2013-11-15
申请号：KR1020110100569
申请日：2011-10-04
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 중탄산염을 포함하는 염소 흔들이병 치료용 약학적 조성물에 관한 것이다.
본 발명의 중탄산염을 포함하는 염소 흔들이병 치료용 약학적 조성물은 염소 흔들이병에 걸린 염소의 치료에 탁월한 효과를 가지며, 또한 이를 경구적으로 염소에 투여함으로써 집단사육을 하는 염소에 대해 투약이 용이하므로 산업상 이용가능성이 있다.-
Patent Agency Ranking24.发明授权하이브리도마 세포주, 이에 의해 생산된 단클론항체,구제역 진단 시약, 진단 키트 및 구제역 바이러스 중화항체검출방법 有权杂交细胞系，从杂交细胞系产生的单克隆抗体，鱼和病毒病毒感染检测试剂，FMDV检测试剂盒和FMDV中和抗体的检测方法
公开(公告)号：KR101102841B1
公开(公告)日：2012-01-05
申请号：KR1020080060980
申请日：2008-06-26
Applicant: 주식회사 메디안디노스틱 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 구제역 바이러스의 O형, A형 및 Asia 1형에 공통적인 면역반응성을 나타내는 단클론항체를 생산하는 하이브리도마 세포주, 구제역 바이러스 O형 VP1 사이트 A에 대한 특이적인 면역반응성과 바이러스 중화능을 나타내는 단클론항체를 생산하는 하이브리도마 세포주, 구제역 바이러스 A형 VP1 사이트 A에 대한 특이적인 면역반응성과 바이러스 중화능을 나타내는 단클론항체를 생산하는 하이브리도마 세포주, 상기 각 하이브리도마 세포주에 의해 생산되는 단클론항체, 상기 단클론항체를 포함하는 구제역 진단 시약, 상기 구제역 진단 시약을 포함하는 구제역 진단 키트 및 구제역 바이러스 중화항체 검출방법 또는 구제역 진단방법에 관한 것이다.
본 발명에 의하면 구제역 바이러스 혈청형에 대한 각각의 항체를 이용하여 구제역 바이러스 진단용 항원을 포획할 필요가 없고, 유전자 재조합 구제역 바이러스 구조단백질을 진단용 항원으로 사용함으로써 종래 차폐시설을 갖춘 실험실에서만 배양이 가능한 구제역 바이러스를 사용하던 방법에 비해 매우 편리하며, 구제역 바이러스 혈청형에 특이적인 단클론항체를 사용하여 각 축종별 이차항체를 따로 사용하는 번거로움 없이 중화항체 검출이 가능하다. 그리고 본 발명의 진단방법은 종래 기술에 비해 검출 민감도와 특이도가 우수하다.
구제역 바이러스, 하이브리도마, 단클론항체, 중화항체, 진단 시약, 진단 키트, 진단방법, 유전자 재조합 구조단백질-
25.发明公开
公开(公告)号：KR1020100006868A
公开(公告)日：2010-01-22
申请号：KR1020080067171
申请日：2008-07-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A diagnostic kit for rapid detection of rabies antibodies is provided to rapidly detect the existence according to rabies vaccination within 20 minutes without expensive equipment and to reduce the generation of rabies. CONSTITUTION: A diagnostic kit for rapid detection of rabies antibodies using immunochromatography has a nitrocellulose membrane(30) containing a test line(40) and a control line(50) on a backing card(70). A conjugate pad is overlapped at one side of the nitrocellulose membrane. A sample pad(10) is overlapped on the conjugate pad. An adsorbent pad(60) absorbing blood serum in which the reaction is completed on a backing card is installed at the other side of the nitrocellulose membrane.
Abstract translation: 目的：提供用于快速检测狂犬病抗体的诊断试剂盒，用于在20分钟内快速检测狂犬病疫苗的存在，无需昂贵的设备，并减少狂犬病的产生。 构成：用于使用免疫层析法快速检测狂犬病抗体的诊断试剂盒具有在背板（70）上含有测试线（40）和对照线（50）的硝酸纤维素膜（30）。 缀合垫在硝酸纤维素膜的一侧重叠。 样品垫（10）重叠在共轭垫上。 在硝酸纤维素膜的另一侧安装吸收血清的吸附垫（60），其中在背衬卡上完成反应。
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26.发明公开하이브리도마 세포주, 이에 의해 생산된 단클론항체,구제역 진단 시약, 진단 키트 및 구제역 바이러스 중화항체검출방법 有权杂交细胞系，从混合细胞系产生的单克隆抗体，FOOD-AND-MOUTH DISEASE VIRUS（FMDV）检测试剂，FMDV检测试剂盒和FMDV中和抗体的检测方法
公开(公告)号：KR1020100001169A
公开(公告)日：2010-01-06
申请号：KR1020080060980
申请日：2008-06-26
Applicant: 주식회사 메디안디노스틱 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A hybridoma cell line producing monoclonal antibody to foot-and-mouth disease virus and a method for detecting foot-and-mouth disease virus neutralization antibody are provided to ensure detection convenience without capture of antigen. CONSTITUTION: A hybridoma cell line(KCTC 11337BP) produces monoclonal antibody having common immunoreactivity to virus type O, A and Asia 1. A method for detecting foot-and-mouth disease virus neutralization antibody comprises: a step of fixing monoclonal antibody on a solid support; a step of washing to remove antibodies which are not fixed on the solid support; a step of reacting antigen for diagnosing foot-and-mouth disease virus on the solid support; a step of washing the antigens to remove antigens which are not bound to the solid support; and a step of reacting probe-conjugated monoclonal antibody with the antigen.
Abstract translation: 目的：提供产生口蹄疫病毒单克隆抗体的杂交瘤细胞系和口蹄疫病毒中和抗体检测方法，以确保检测方便而无需捕获抗原。 构成：杂交瘤细胞系（KCTC 11337BP）产生对病毒O，A和亚洲1具有常见免疫反应性的单克隆抗体。一种用于检测口蹄疫病毒中和抗体的方法，包括：将单克隆抗体固定在固体上的步骤 支持; 洗涤步骤以除去未固定在固体支持物上的抗体; 将抗原用于诊断口蹄疫病毒的固体支持物的步骤; 洗涤抗原以除去不与固体支持物结合的抗原的步骤; 和探针结合的单克隆抗体与抗原反应的步骤。
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公开(公告)号：KR100449906B1
公开(公告)日：2004-09-22
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组载体通过克隆编码 重组FMCV 2C非结构蛋白SEQ ID NO：1。通过用重组载体转化细胞表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组FMCV 2C非结构蛋白特异性单克隆抗体稀释于包被缓冲液中，并将稀释液倒入平板中; （2）清洗平板以去除未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与平板反应; （4）洗涤平板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与平板反应; （6）洗涤平板以除去未反应的测试血清; （7）使与试验血清中的口蹄疫病毒抗体结合的缀合物与试验血清具有酶，放射性物质或荧光物质的步骤; 和（8）测量酶反应的强度，与缀合物的荧光反应或辐射反应。
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公开(公告)号：KR1020030052859A
公开(公告)日：2003-06-27
申请号：KR1020010082977
申请日：2001-12-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/33
CPC classification number: C12N5/163 , C07K16/1009 , C07K2317/14 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
Abstract: PURPOSE: A diagnostic method of foot-and-mouth disease using a recombinant FMCV 2C non-structural protein and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant FMCV 2C non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant vector is prepared by cloning the gene encoding the recombinant FMCV 2C non-structural protein of SEQ ID NO: 1. The recombinant FMCV 2C non-structural protein is expressed by transformation of a cell with the recombinant vector. A hybridoma cell line(KCTC 10137BP) is prepared by introducing the recombinant FMCV 2C non-structural protein into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A recombinant FMCV 2C non-structural protein specific monoclonal antibody is produced from the hybridoma cell line(KCTC 10137BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant FMCV 2C non-structural protein specific monoclonal antibody in a coating buffer solution and pouring the diluate on a plate; (2) washing the plate to remove unattached monoclonal antibodies; (3) reacting the recombinant FMCV 2C non-structural protein with the plate; (4) washing the plate to remove unreacted recombinant FMCV 2C non-structural proteins; (5) reacting the testing serum with the plate; (6) washing the plate to remove unreacted testing serum; (7) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (8) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
Abstract translation: 目的：提供使用重组FMCV 2C非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组FMCV 2C非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。通过克隆编码 SEQ ID NO：1的重组FMCV 2C非结构蛋白。通过用重组载体转化细胞来表达重组FMCV 2C非结构蛋白。 通过将重组FMCV 2C非结构蛋白引入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合，来制备杂交瘤细胞系（KCTC 10137BP）。 从杂交瘤细胞系（KCTC 10137BP）产生重组FMCV 2C非结构蛋白特异性单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包衣缓冲溶液中稀释重组FMCV 2C非结构蛋白特异性单克隆抗体，将稀释物倒入板上; （2）洗涤板以除去未附着的单克隆抗体; （3）使重组FMCV 2C非结构蛋白与板反应; （4）洗涤板以除去未反应的重组FMCV 2C非结构蛋白; （5）使测试血清与板反应; （6）洗板以除去未反应的检测血清; （7）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（8）测量酶反应的强度，荧光反应或与缀合物的辐射反应。
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